Citation: Jun YAO, Yi LIU, Tian Zhi WANG, Jian Ben LIU, Peng LIU, Zi Niu YU. Microcalorimetric Study on Tyrosine Oxidation Catalyzed by Tyrosinase[J]. Chinese Chemical Letters, ;2003, 14(6): 619-622. shu

Microcalorimetric Study on Tyrosine Oxidation Catalyzed by Tyrosinase

Jun YAO ^1,3, ,
Yi LIU ² ,
Tian Zhi WANG ² ,
Jian Ben LIU ³ ,
Peng LIU ² ,
Zi Niu YU ¹

1.
Key Laboratory of Microbiology of Chinese Agricultural Ministry, Huazhong Agricultural University, Wuhan 430070;
2.
College of Chemistry and Molecular Science, Wuhan University, Wuhan 430072;
3.
College of Life Science and Chemistry, Jishou University, Jishou 416000

Received Date: 25 July 2002

Fund Project: This work were supported by the National Natural Science Foundation of China (No. 30170010, 29973030), and The Teaching and Research Award Program for Outstanding Young Professors in High Education Institute, MOE, China (2001).

Through the method of initial heat release rate, the kinetic property of tyrosine oxidationcatalyzed by tyrosinase from Pseudomonas maltophilia was investigated using a LKB-2107 batchmicrocalorimeter. Tyrosine was catalyzed and oxidized into L-dopa, then into melanin catalyzed bytyrosinase. We found that the tyrosinase reaction obeyed the Michaelis-Menten kinetics, and at298.15K and pH 7.0, the initial exothermic rate (Ω₀) are in the range of0.1567~0.5704 mJ·s^-1, the maximum exothermic rate (Ω_max) are in 0.4152~0.8143mol·L^-1, and mean value of the Michaelisconstant (K_m) is 2.199±0.105×10⁴ mol·L^-1.
- Tyrosine,
- tyrosinase-catalyzed oxidation reaction,
- microcalorimetry,
- thermokinetics
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