Citation: Hong LIANG, Xing Can SHEN, Fang LI, Zhi Liang JIANG. The Photochemical Study of HSA and BSA with Resonance Light-Scattering and Fluorescence Spectra[J]. Chinese Chemical Letters, ;2000, 11(3): 251-254. shu

The Photochemical Study of HSA and BSA with Resonance Light-Scattering and Fluorescence Spectra

Institute of Bioinorganic Chemistry, Guangxi Normal University, Guilin 541004

Received Date: 28 June 1999

Fund Project: This project is supported by the National Natural Science Foundation of China (NO.29961001) and the Foundation for Talents Striding across the Century of Guangxi.

The resonance light-scattering (RLS) of human serum albumin (HSA) and bovine serum albumin (BSA) is reported for the first time,and applied to study photochemical reaction of HSA and BSA. The fact of photocrosslinking self-association effect in HSA and BSA solutions is identified by the enhancement of RLS. The fluorescence quenching at about 350 nm and 700 nm proves that tryptophan (Trp) residues are one of the photochemical activity sites in HSA and BSA molecules. The Rayleigh scattering (RS) spectra of HSA and BSA that were neglected in fluorescence spectra before are found at about 296 nm,592 nm and 888 nm for the first time,and are of adventageous to studying the aggregation of HSA or BSA. The possible photochemical reaction mechanism is also proposed.
- Photochemical reaction,
- photocrosslinking self-association effect,
- resonance light-scattering,
- serum albumin
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