Citation: Panpan Sun, Qian Li, Ningshuang Gao, Mingyue Luo, Wenzhuo Chang, Baodui Wang, Xiaoquan Lu, Zhonghua Xue. Solid state luminescent-enabled lateral flow immunoassay with highly fluorescence performance for rapid and quantitative detection of C-reactive protein[J]. Chinese Chemical Letters, ;2025, 36(10): 110801. doi: 10.1016/j.cclet.2024.110801 shu

Solid state luminescent-enabled lateral flow immunoassay with highly fluorescence performance for rapid and quantitative detection of C-reactive protein

Panpan Sun ^{a, 1} ,
Qian Li ^{a, 1} ,
Ningshuang Gao ^b ,
Mingyue Luo ^a ,
Wenzhuo Chang ^a ,
Baodui Wang ^b ,
Xiaoquan Lu ^a ,
Zhonghua Xue ^{a, *,}

a.
Key Laboratory of Water Security and Water Environment Protection in Plateau Intersection (NWNU), Ministry of Education; Key Lab of Bioelectrochemistry and Environmental Analysis of Gansu Province, College of Chemistry and Chemical Engineering, Northwest Normal University, Lanzhou 730070, China
b.
State Key Laboratory of Applied Organic Chemistry and Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province, Lanzhou University, Lanzhou 730000, China

xzhlab@hotmail.com

Received Date: 18 October 2024
Revised Date: 23 December 2024
Accepted Date: 25 December 2024
Available Online: 25 December 2024

Figures(5)

The advancement of various types of fluorescent nanoparticles is crucial for enhancing the application of lateral flow immunoassays (LFIA) across multiple fields. Currently, the fluorescent nanoparticles utilized in LFIA predominantly consist of traditional dye-doped nanoparticles or aggregation-induced luminescence dye-doped nanoparticles. The reliance on specific types of nanoparticles limits the diversity of signal reporting groups available for LFIA. Herein, we developed a solid-state luminescent dye-doped nanoparticles (SLDNPs)-based LFIA system with exceptional stability for the detection of C-reactive protein (CRP) in serum. The synthesis of SLD₅₂₀NP_S was simplicity, efficient and eco-friendly, which was ideal for large-scale production of the LFIA test strip. And the SLD₅₂₀NP_S exhibits superior fluorescence quantum yield (49%), fully guarantees the performance of the LFIA test strip. The constructed SLD₅₂₀NPs-mAb1-based LFIA demonstrated a satisfactory linear relationship with CRP concentrations ranging from 0.5 ng/mL to 100 ng/mL, with limits of detection (LOD) of 0.78 ng/mL and a visible LOD of 1 ng/mL using a handheld 405 nm lamp. Furthermore, the developed LFIA exhibited excellent recoveries in serum, ranging from 94.45% to 102.5%. Overall, the outstanding performance of the SLD₅₂₀NPs-mAb1-based LFIA indicates that solid-state luminescent dyes have significant potential applications in the field of LFIA.
- Point-of-care testing,
- Lateral flow immunoassay,
- Fluorescent nanoparticles,
- Early detection,
- C-reactive protein,
- Solid-state luminescent
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