Citation: Lian Jin, Juan Zhang, Libo Nie, Yan Deng, Ghulam Jilany Khana, Nongyue He. Chitosan nanoparticles act as promising carriers of microRNAs to brain cells in neurodegenerative diseases[J]. Chinese Chemical Letters, ;2025, 36(10): 110774. doi: 10.1016/j.cclet.2024.110774 shu

Chitosan nanoparticles act as promising carriers of microRNAs to brain cells in neurodegenerative diseases

Lian Jin ^{a, f} ,
Juan Zhang ^{a, f} ,
Libo Nie ^{a, *,} ,
Yan Deng ^{b, c, *,} ,
Ghulam Jilany Khana ^e ,
Nongyue He ^{a, d, *,}

a.
Hunan Key Laboratory of Biomedical Nanomaterials and Devices, Hunan University of Technology, Zhuzhou 412007, China
b.
Institute for Future Sciences, University of South China, Changsha 410000, China
c.
Institute of Cytology and Genetics, School of Basic Medical Sciences, Hengyang Medical School, University of South China, Hengyang 421001, China
d.
State Key Laboratory of Digital Medical Engineering, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China
e.
Faculty of Pharmaceutical Sciences, University of Central Punjab, 1-Khyaban-e-Jinah Road, Johar Town, Lahore 54000, Pakistan
f.
Shenzhen Feidu Biomedicine Co., Ltd., Shenzhen 518116, China

nielibo@hut.edu.cn

hndengyan@126.com

nyhe1958@163.com

Received Date: 2 November 2024
Revised Date: 12 December 2024
Accepted Date: 17 December 2024
Available Online: 18 December 2024

Figures(5)

MicroRNAs (miRNAs) are abundant in the brain and mounting evidence suggests their involvement in the critical processes such as neurodevelopment, synaptic plasticity, and the development of neurodegenerative diseases. Thus, miRNAs may be promising therapeutic drugs for the treatment of neurodegenerative disorders. However, naked miRNAs are not able to enter cells directly, especially brain cells. Therefore, suitable carriers for safe and efficient miRNA delivery to brain cells are of great importance. Chitosan nanoparticles, with the excellent properties such as good compatibility and brilliant degradability, may act as a promising carrier for miRNA drug delivery. In this study, chitosan nanoparticles were prepared and their properties such as particle size, zeta potential and encapsulation efficiency were optimized to encapsulate miRNAs. The delivery efficiency of miRNA-loaded nanoparticles was then evaluated in both neuronal and microglia cells. The results demonstrated chitosan nanoparticles encapsulated miRNAs efficiently and showed excellent sustained releasing in vitro. Moreover, chitosan nanoparticles delivered miRNA to both neurons and microglia with very low toxicity and high efficiency. In conclusion, chitosan nanoparticles are promising carriers for the delivery of miRNAs to brain cells, which may be used for the early intervention and treatment of neurodegenerative disorders.
- Chitosan,
- Nanoparticles,
- Drug delivery,
- MicroRNA,
- Neurodegenerative diseases,
- Neuron,
- Microglia
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沈阳化工大学材料科学与工程学院沈阳 110142