Citation: Jielin Wang, Han Ye, Bozhuang Zhou, Zhen Pan, Yucai Li, Zhenyuan Wei, Bin Chai, Yizhou Gao, Xiaojian Ye, Jiangming Yu. Biomimetic nanofibrillar/hyaluronic acid hydrogels remodel the neuromodulatory microenvironment for enhanced bone regeneration[J]. Chinese Chemical Letters, ;2025, 36(5): 110133. doi: 10.1016/j.cclet.2024.110133 shu

Biomimetic nanofibrillar/hyaluronic acid hydrogels remodel the neuromodulatory microenvironment for enhanced bone regeneration

Jielin Wang ^{a, b, c, 1,} ,
Han Ye ^{d, 1} ,
Bozhuang Zhou ^e ,
Zhen Pan ^f ,
Yucai Li ^f ,
Zhenyuan Wei ^f ,
Bin Chai ^f ,
Yizhou Gao ^{e, *,} ,
Xiaojian Ye ^{a, b, c, f, *,} ,
Jiangming Yu ^{a, b, f, *,}

a.
Laboratory of Key Technology and Materials in Minimally Invasive Spine Surgery, Tongren Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200336, China
b.
Shanghai Jiao Tong University Spinal Minimally Invasive Research Center, Tongren Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200336, China
c.
Hongqiao International Institute of Medicine, Tongren Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200336, China
d.
Department of Ophthalmology and Vision Science, Shanghai Eye Ear Nose and Throat Hospital, Fudan University, Shanghai 200126, China
e.
The Center for Microbes Development and Health, Shanghai Insititute of Immunity and Infection Chineses Academy of Science, Shanghai 200031, China
f.
Department of Orthopaedic, Tongren Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200336, China

wangjielin369@126.com

gaoyizhou@126.com

yexj2002@163.com

yjm_st@163.com

Received Date: 2 April 2024
Revised Date: 13 June 2024
Accepted Date: 17 June 2024
Available Online: 17 June 2024

Figures(4)

Severe traumatic bone healing relies on the involvement of growth factors. However, excessive supplementation of growth factors can lead to ectopic ossification and inflammation. In this study, utilizing the neural regulatory mechanism of bone regeneration, we have developed a multifunctional three dimensions (3D) printed scaffold containing both vasoactive intestinal peptide (VIP) and nerve growth factor (NGF) as an effective new method for achieving bone defect regeneration. The scaffold is provided by a controlled biodegradable and biomechanically matched poly(lactide-ethylene glycol-trimethylene carbonate) (PLTG), providing long-term support for the bone healing cycle. Factor loading is provided by peptide fiber-reinforced biomimetic antimicrobial extracellular matrix (ECM) (B-ECM) hydrogels with different release kinetics, the hydrogel guides rapid bone growth and resists bacterial infection at the early stage of healing. Physical and chemical characterization indicates that the scaffold has good structural stability and mechanical properties, providing an ideal 3D microenvironment for bone reconstruction. In the skull defect model, compared to releasing VIP or NGF alone, this drug delivery system can simulate a natural healing cascade of controllable release factors, significantly accelerating nerve/vascular bone regeneration. In conclusion, this study provides a promising strategy for implanting materials to repair bone defects by utilizing neuroregulatory mechanisms during bone regeneration.
- Neuroregulation,
- Vasoactive intestinal peptide,
- Nerve growth factor,
- Biomimetic extracellular matrix,
- Bone regeneration
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