Citation: Jia-Li Xie, Tian-Jin Xie, Yu-Jie Luo, Kai Mao, Cheng-Zhi Huang, Yuan-Fang Li, Shu-Jun Zhen. Octopus-like DNA nanostructure coupled with graphene oxide enhanced fluorescence anisotropy for hepatitis B virus DNA detection[J]. Chinese Chemical Letters, ;2024, 35(6): 109137. doi: 10.1016/j.cclet.2023.109137 shu

Octopus-like DNA nanostructure coupled with graphene oxide enhanced fluorescence anisotropy for hepatitis B virus DNA detection

a.
Key Laboratory of Luminescence Analysis and Molecular Sensing (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China
b.
Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University), Chongqing Science and Technology Bureau, College of Pharmaceutical Sciences, Southwest University, Chongqing 400715, China

zsj@swu.edu.cn

Received Date: 11 May 2023
Revised Date: 4 September 2023
Accepted Date: 21 September 2023
Available Online: 25 September 2023

Figures(6)

Fluorescence Anisotropy (FA) is an effective biochemical detection method based on molecular rotations. Graphene oxide (GO) has been extensively used as an FA amplifier. However, the enhancement of FA by GO alone is limited and the strong scattering of GO will easily make the measurement of FA inaccurate. In order to address these problems, an octopus-like DNA nanostructure (ODN) was designed and coupled with GO to enhance the FA together in this work. By mimicking the multi-clawed structure of the octopus, the ODN can be adsorbed on GO tightly, which not only could improve the sensitivity because of the double FA enhancement abilities of GO and ODN, but also could improve the specificity due to the decrease of the nonspecific interaction in complex samples. Furthermore, ODN could maintain a certain distance between the fluorophore and GO to reduce the fluorescence quenching efficiency of GO, which could improve the accuracy. This method has been applied for the detection of hepatitis B virus DNA (HBV-DNA) in a range of 1–50nmol/L and the limit of detection (LOD) was 330pmol/L. In addition, the proposed method has been successfully utilized to detect HBV-DNA in human serum, indicating that this method has a great practical application prospect.
- Fluorescence anisotropy,
- Graphene oxide (GO),
- Octopus-like DNA nanostructure,
- HBV-DNA
1. [1]
  D. Zhang, M. Lu, H. Wang, J. Am. Chem. Soc. 133 (2011) 9188–9191. doi: 10.1021/ja202141y
2. [2]
  X. Xiao, S. Zhen, RSC Adv. 12 (2022) 6364–6376. doi: 10.1039/d2ra00058j
3. [3]
  K. Chullipalliyalil, K. Elkassas, M.A.P. McAuliffe, S. Vucen, A. Crean, Anal. Chem. 95 (2023) 2774–2782. doi: 10.1021/acs.analchem.2c03912
4. [4]
  D. Zhang, H. Wang, Anal. Chem. 91 (2019) 14538–14544. doi: 10.1021/acs.analchem.9b03556
5. [5]
  W. Huang, C. Guo, J. Zhai, X. Xie, Anal. Chem. 94 (2022) 9793–9800. doi: 10.1021/acs.analchem.2c01532
6. [6]
  Q. Huang, B. Chen, J. Shen, et al., J. Am. Chem. Soc. 143 (2021) 10735–10742. doi: 10.1021/jacs.1c04942
7. [7]
  J. Chen, J. Liu, X. Chen, H. Qiu, Chin. Chem. Lett. 30 (2019) 1575–1580. doi: 10.1016/j.cclet.2019.06.005
8. [8]
  S. Jain, A. Heiser, A.R. Venter, Analyst 136 (2011) 1298–1301. doi: 10.1039/c0an00728e
9. [9]
  T.L. Mann, U.J. Krull, Analyst 128 (2003) 313–317. doi: 10.1039/b300873h
10. [10]
  Y.L. Fan, Z.Y. Liu, Y.M. Zeng, et al., Talanta 223 (2021) 121721. doi: 10.1016/j.talanta.2020.121721
11. [11]
  B. Yang, X.B. Zhang, L.P. Kang, et al., Anal. Chem. 85 (2013) 11518–11523. doi: 10.1021/ac402781g
12. [12]
  C. Zeng, J. Gao, Y. Lou, L. Cui, Talanta 218 (2020) 121179. doi: 10.1016/j.talanta.2020.121179
13. [13]
  M. Zou, Y. Chen, X. Xu, et al., Biosens. Bioelectron. 32 (2012) 148–154. doi: 10.1016/j.bios.2011.11.052
14. [14]
  A. Pepanian, P. Sommerfeld, R. Kasprzyk, et al., Anal. Chem. 94 (2022) 14410–14418. doi: 10.1021/acs.analchem.2c03176
15. [15]
  B.C. Yin, P. Zuo, H. Huo, X. Zhong, B.C. Ye, Anal. Biochem. 401 (2010) 47–52. doi: 10.1016/j.ab.2010.02.014
16. [16]
  Y. Yu, Y. Liu, S.J. Zhen, C.Z. Huang, Chem. Commun. 49 (2013) 1942–1944. doi: 10.1039/c3cc38129c
17. [17]
  B.C. Ye, B.C. Yin, Angew. Chem. Int. Ed. 47 (2008) 8386–8389. doi: 10.1002/anie.200803069
18. [18]
  J. Zhang, J. Tian, Y. He, Y. Zhao, S. Zhao, Chem. Commun. 50 (2014) 2049–2051. doi: 10.1039/C3CC49424A
19. [19]
  X. Xiao, J. Tao, H.Z. Zhang, C.Z. Huang, S.J. Zhen, Biosens. Bioelectron. 85 (2016) 822–827. doi: 10.1016/j.bios.2016.05.091
20. [20]
  Y.X. Liu, X. Xiao, C.H. Li, et al., Talanta 211 (2020) 120730. doi: 10.1016/j.talanta.2020.120730
21. [21]
  Q. Zhao, Q. Lv, H. Wang, Anal. Chem. 86 (2014) 1238–1245. doi: 10.1021/ac4035532
22. [22]
  Y. Li, H. Yu, Q. Zhao, RSC Adv. 12 (2022) 7464–7468. doi: 10.1039/d2ra00843b
23. [23]
  C. Hoffmann, M. Jourdain, A. Grandjean, A. Titz, G. Jung, Anal. Chem. 94 (2022) 6112–6119. doi: 10.1021/acs.analchem.1c04654
24. [24]
  X. Xiao, Y.F. Li, C.Z. Huang, S.J. Zhen, Chem. Commun. 51 (2015) 16080–16083. doi: 10.1039/C5CC05902J
25. [25]
  S.J. Zhen, X. Xiao, C.H. Li, C.Z. Huang, Anal. Chem. 89 (2017) 8766–8771. doi: 10.1021/acs.analchem.7b00955
26. [26]
  B. Billet, B. Chovelon, E. Fiore, et al., Angew. Chem. Int. Ed. 60 (2021) 12346–12350. doi: 10.1002/anie.202102254
27. [27]
  L. Gao, L. Liu, Y. Tian, et al., Anal. Chem. 93 (2021) 7045–7053. doi: 10.1021/acs.analchem.1c00363
28. [28]
  E. Sijbesma, B.A. Somsen, G.P. Miley, et al., ACS Chem. Biol. 15 (2020) 3143–3148. doi: 10.1021/acschembio.0c00646
29. [29]
  Q.H. Wan, X.C. Le, Anal. Chem. 72 (2000) 5583–5589. doi: 10.1021/ac000318+
30. [30]
  Y. Li, L. Sun, Q. Zhao, Talanta 174 (2017) 7–13. doi: 10.1016/j.talanta.2017.05.077
31. [31]
  Y. Li, Q. Zhao, Anal. Chem. 91 (2019) 7379–7384. doi: 10.1021/acs.analchem.9b01253
32. [32]
  Z. Zhu, T. Schmidt, M. Mahrous, et al., Anal. Chim. Acta 707 (2011) 191–196. doi: 10.1016/j.aca.2011.09.022
33. [33]
  S. Perrier, P. Bouilloud, G. De Oliveira Coelho, M. Henry, E. Peyrin, Biosens. Bioelectron. 82 (2016) 155–161. doi: 10.1016/j.bios.2016.04.010
34. [34]
  Y. Huang, S. Zhao, Z.F. Chen, Y.C. Liu, H. Liang, Chem. Commun. 47 (2011) 4763–4765. doi: 10.1039/c1cc10325c
35. [35]
  N. Mohanty, V. Berry, Nano Lett. 8 (2008) 4469–4476. doi: 10.1021/nl802412n
36. [36]
  F. Kim, L.J. Cote, J. Huang, Adv. Mater. 22 (2010) 1954–1958. doi: 10.1002/adma.200903932
37. [37]
  Y. Hu, F. Li, D. Han, et al., Anal. Chim. Acta. 753 (2012) 82–89. doi: 10.1016/j.aca.2012.09.038
38. [38]
  Y. Piao, F. Liu, T.S. Seo, Chem. Commun. 47 (2011) 12149–12151. doi: 10.1039/c1cc15043j
39. [39]
  T. Miyahata, Y. Kitamura, A. Futamura, et al., Chem. Commun. 49 (2013) 10139–10141. doi: 10.1039/c3cc45531a
40. [40]
  L. Gao, C. Lian, Y. Zhou, et al., Biosens. Bioelectron. 60 (2014) 22–29. doi: 10.1016/j.bios.2014.03.039
41. [41]
  Q. Tang, Q. Zhang, Y. Jiang, et al., ACS Appl. Mater. Inter. 6 (2014) 13470–13477. doi: 10.1021/am502311b
42. [42]
  A.E. Prigodich, O.S. Lee, W.L. Daniel, et al., J. Am. Chem. Soc. 132 (2010) 10638–10641. doi: 10.1021/ja104859j
43. [43]
  Y. Chen, D. Tyagi, M. Lyu, et al., Anal. Chem. 91 (2019) 4017–4022. doi: 10.1021/acs.analchem.8b05432
44. [44]
  G. Kang, C.A.H. Allard, W.A. Valencia-Montoya, et al., Nature 616 (2023) 378–383. doi: 10.1038/s41586-023-05808-z
45. [45]
  J.H. Choi, M. Shin, L. Yang, et al., ACS Nano 15 (2021) 13475–13485. doi: 10.1021/acsnano.1c03975
46. [46]
  Y. Liang, W.L. Ang, R.R.X. Lim, A. Bonanni, Chem. Commun. 58 (2022) 2662–2665. doi: 10.1039/d1cc06657a
47. [47]
  K. Ma, W. Xie, W. Liu, et al., Adv. Funct. Mater. 31 (2021) 2102645. doi: 10.1002/adfm.202102645
48. [48]
  J. Vilcapoma, A. Patel, A.R. Chandrasekara, K. Halvorsen, Chem. Commun. 59 (2023) 5083–5085. doi: 10.1039/d3cc01123b
49. [49]
  Y.Z. Guo, J.L. Liu, Y.F. Chen, et al., Anal. Chem. 94 (2022) 7601–7608. doi: 10.1021/acs.analchem.2c00763
50. [50]
  S. Pfister, J. Rabl, T. Wiegand, et al., Nat. Commun. 14 (2023) 1574. doi: 10.1038/s41467-023-37068-w
51. [51]
  J. Su, W. Liu, S. Chen, et al., ACS Sens. 5 (2020) 3979–3987. doi: 10.1021/acssensors.0c01745
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