Citation: Xin Lu, Haoran Sun, Xiaomeng Li, Chunrui Li, Jinfeng Wang, Dandan Zhou. C14-HSL limits the mycelial morphology of pathogen Trichosporon cells but enhances their aggregation: Mechanisms and implications[J]. Chinese Chemical Letters, ;2024, 35(6): 108936. doi: 10.1016/j.cclet.2023.108936 shu

C14-HSL limits the mycelial morphology of pathogen Trichosporon cells but enhances their aggregation: Mechanisms and implications

Xin Lu ^a ,
Haoran Sun ^a ,
Xiaomeng Li ^a ,
Chunrui Li ^a ,
Jinfeng Wang ^b ,
Dandan Zhou ^{a, *,}

a.
Jilin Engineering Lab for Water Pollution Control and Resources Recovery, School of the Environment, Northeast Normal University, Changchun 130117, China
b.
State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210023, China

zhoudandan415@163.com

Received Date: 25 April 2023
Revised Date: 10 August 2023
Accepted Date: 14 August 2023
Available Online: 18 August 2023

Figures(5)

The biosecurity hazards caused by pathogenic fungus have been widely concerned. Given the long-term coexistence of eukaryotic pathogens and quorum sensing bacteria in different habitats in environments, we hypothesized that they have social interactions via signal molecules. In this work, we firstly discovered the well-known bacterial signal molecules play an adverse role in the cell morphology and metabolism in a model pathogen Trichosporon asahii. N-Tetradecanoyl-L-homoserine lactone (C14-HSL) was discovered to increase pathogen hazards of T. asahii, which limited mycelium by 52%, but enhanced cell aggregation by 93%. Higher fluorescence intensity of tryptophan (59%) and aromatic protein (2-fold) contents after the treatment of C14-HSL, indicating that aromatic proteins helped aggregate Trichosporon and showed hydrophobicity. Transcriptome analysis revealed that C14-HSL upregulated the shikimate pathway (above 1-fold) located in downstream of tricarboxylic acid cycle, which contributed to the synthesis of more aromatic proteins and the formation of larger flocs. The limited mycelial growth of T. asahii attributed to the up-regulated expressions of cell cycle process. The fungal transboundary response to bacterial C14-HSL was controlled by signal transduction pathways. This study provides new insights into the co-evolution of bacterial and pathogenic fungi in microecosystems.
- Bacterial C14-HSL,
- Mycelium,
- Cell cycle,
- Aggregation,
- Aromatic proteins
1. [1]
  J. Sun, H. Sun, W. Lv, et al., J. Environ. Chem. Eng. 9 (2021) 105817. doi: 10.1016/j.jece.2021.105817
2. [2]
  R.A. Cordeiro, A.L.R. Aguiar, V.S. Pereira, et al., Microb. Pathog. 130 (2019) 219–225. doi: 10.1016/j.micpath.2019.03.013
3. [3]
  H.X. Zhao, T.Y. Zhang, H. Wang, et al., Sci. Total Environ. 853 (2022) 158626. doi: 10.1016/j.scitotenv.2022.158626
4. [4]
  A.L. Colombo, A.C.B. Padovan, G.M. Chaves, et al., Clin. Microbiol. Rev. 24 (2011) 682–700. doi: 10.1128/CMR.00003-11
5. [5]
  E.R. Galligan, L. Fix, S. Husain, et al., J. Cutan. Pathol. 46 (2019) 159–161. doi: 10.1111/cup.13397
6. [6]
  A. Subramanian, S. Devi, G. Abraham, et al., Med. Mycol. Case Rep. 35 (2022) 15–17. doi: 10.1016/j.mmcr.2021.12.001
7. [7]
  T.L. Han, R.D. Cannon, S.G. Villas-Bôas, Fungal Genet. Biol. 48 (2011) 747–763. doi: 10.1016/j.fgb.2011.04.002
8. [8]
  G.D. Bonaventura, A. Pompilio, C. Picciani, et al., Antimicrob. Agents Chemother. 50 (2006) 3269–3276. doi: 10.1128/AAC.00556-06
9. [9]
  X. Lu, Y. Wang, Z. Feng, et al., Chin. Chem. Lett. 34 (2023) 107617. doi: 10.1016/j.cclet.2022.06.040
10. [10]
  J. Shuai, X. Hu, B. Wang, et al., Chin. Chem. Lett. 32 (2021) 3402–3409. doi: 10.1016/j.cclet.2021.04.061
11. [11]
  S. Li, P.H.M. Leung, X. Xu, C. Wu, Chin. Chem. Lett. 29 (2018) 313–316. doi: 10.1016/j.cclet.2017.09.052
12. [12]
  L.D. Sordi, F.A. Mühlschlegel, FEMS Yeast Res. 9 (2009) 990–999. doi: 10.1111/j.1567-1364.2009.00573.x
13. [13]
  A. Davis-Hanna, A.E. Piispanen, L.I. Stateva, et al., Mol. Microbiol. 67 (2008) 47–62. doi: 10.1111/j.1365-2958.2007.06013.x
14. [14]
  J. Barriuso, D.A. Hogan, T. Keshavarz, et al., FEMS Microbiol. Rev. 42 (2018) 627–638. doi: 10.1093/femsre/fuy022
15. [15]
  C. Yang, S. Fang, D. Chen, et al., Mar. Pollut. Bull. 107 (2016) 118–124. doi: 10.1016/j.marpolbul.2016.04.010
16. [16]
  F. Getzke, T. Thiergart, S. Hacquard, Curr. Opin. Microbiol. 49 (2019) 66–72. doi: 10.1016/j.mib.2019.10.009
17. [17]
  Y. Liao, H. Zhao, X. Lu, et al., Med. Mycol. 53 (2015) 396–404. doi: 10.1093/mmy/myv006
18. [18]
  G. Gillot, N. Decourcelle, G. Dauer, et al., Food Microbiol. 57 (2016) 1–7. doi: 10.1016/j.fm.2015.12.008
19. [19]
  H. Fan, X. Liu, H. Wang, et al., Chemosphere. 169 (2017) 586–595. doi: 10.1016/j.chemosphere.2016.10.137
20. [20]
  Y. Sun, K. He, Q. Yin, et al., J. Environ. Sci. China 69 (2018) 85–94. doi: 10.1016/j.jes.2017.04.017
21. [21]
  Y. Shen, D.M. Huang, Y.P. Chen, et al., Sci. Total Environ. 712 (2020) 135795. doi: 10.1016/j.scitotenv.2019.135795
22. [22]
  P. Zhang, F. Fang, Y.P. Chen, et al., Chemosphere 117 (2014) 59–65. doi: 10.1016/j.chemosphere.2014.05.070
23. [23]
  Q. He, J. Zhang, S. Gao, et al., Bioresour. Technol. 294 (2019) 122151. doi: 10.1016/j.biortech.2019.122151
24. [24]
  Z.P. Wang, T. Zhang, Water Res. 44 (2010) 5499–5509. doi: 10.1016/j.watres.2010.06.067
25. [25]
  G.H. Yu, P.J. He, L.M. Shao, Bioresour. Technol. 100 (2009) 3193–3198. doi: 10.1016/j.biortech.2009.02.009
26. [26]
  R.S. Pembrey, K.C. Marshall, R.P. Schneider, Appl. Environ. Microbiol. 65 (1999) 2877–2894. doi: 10.1128/aem.65.7.2877-2894.1999
27. [27]
  C. Strub, C.A.T. Dieye, P.A. Nguyen, et al., Fungal Biol. 125 (2021) 78–88. doi: 10.1016/j.funbio.2019.11.007
28. [28]
  E.K. Palonen, S. Raina, A. Brandt, et al., Microorganisms 5 (2017) E12. doi: 10.3390/microorganisms5010012
29. [29]
  H.L. Kher, T. Krishnan, V. Letchumanan, et al., Gene 684 (2019) 58–69. doi: 10.1016/j.gene.2018.10.031
30. [30]
  A.L. Schaefer, T.A. Taylor, J.T. Beatty, et al., J. Bacteriol. 184 (2002) 6515–6521. doi: 10.1128/JB.184.23.6515-6521.2002
31. [31]
  N. Wang, J. Gao, Y. Liu, et al., Chemosphere 274 (2021) 129970. doi: 10.1016/j.chemosphere.2021.129970
32. [32]
  P.F. Fitzpatrick, Biochemistry 42 (2003) 14083–14091. doi: 10.1021/bi035656u
33. [33]
  Y. Li, W. Hao, J. Lv, et al., Bioresour. Technol. 159 (2014) 305–310. doi: 10.3892/mmr.2013.1793
34. [34]
  M. Pivokonsky, J. Naceradska, T. Brabenec, et al., Water Res. 84 (2015) 278–285. doi: 10.1016/j.watres.2015.07.047
35. [35]
  Y. Wang, H. Xu, H. Yao, et al., J. Hazard. Mater. 443 (2023) 130306. doi: 10.1016/j.jhazmat.2022.130306
36. [36]
  V.K. Gupta, O. Chaudhuri, Trends Cell Biol. 32 (2022) 773–785. doi: 10.1016/j.tcb.2022.03.010
37. [37]
  R. Duronio, Y. Xiong, Cold Spring Harb. Perspect. Biol. 5 (2013) a008904. doi: 10.1101/cshperspect.a008904
38. [38]
  D. Patzelt, H. Wang, I. Buchholz, et al., ISME J. 7 (2013) 2274–2286. doi: 10.1038/ismej.2013.107
39. [39]
  G. Zou, K. Zhang, W. Yang, et al., Chin. Chem. Lett. 32 (2021) 3252–3256. doi: 10.1016/j.cclet.2021.02.036
40. [40]
  J. Frankovsky, V. Vozáriková, J. Nosek, et al., Mitochondrion 57 (2021) 148–162. doi: 10.1016/j.mito.2020.12.016
41. [41]
  Q. Zhang, W. Zeng, S. Xu, et al., Bioresour. Technol. 342 (2021) 125978. doi: 10.1016/j.biortech.2021.125978
42. [42]
  D. Martínez-Soto, J. Ruiz-Herrera, Rev. Iberoam. Micol. 34 (2017) 192–202. doi: 10.1016/j.riam.2017.02.006
43. [43]
  J. Zhou, Y. Lyu, M. Richlen, et al., Crit. Rev. Plant Sci. 35 (2016) 81–105. doi: 10.1080/07352689.2016.1172461
44. [44]
  R. Alonso-Monge, E. Román, D.M. Arana, et al., Clin. Microbiol. Infect. 15 (Suppl. 1) (2009) 17–19. doi: 10.1111/j.1469-0691.2008.02690.x
45. [45]
  S.A. Padder, R. Prasad, A.H. Shah, Microbiol. Res. 210 (2018) 51–58. doi: 10.1016/j.micres.2018.03.007
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