Citation: Cheng Guo, Xiaoxiao Zhang, Xiujuan Hong, Yiqiu Hu, Lingna Mao, Kezhi Jiang. Graphene as adsorbent for highly efficient extraction of modified nucleosides in urine prior to liquid chromatography-tandem mass spectrometry analysis[J]. Chinese Chemical Letters, ;2024, 35(4): 108867. doi: 10.1016/j.cclet.2023.108867 shu

Graphene as adsorbent for highly efficient extraction of modified nucleosides in urine prior to liquid chromatography-tandem mass spectrometry analysis

Cheng Guo ^{a, b, *,} ,
Xiaoxiao Zhang ^{a, c} ,
Xiujuan Hong ^a ,
Yiqiu Hu ^a ,
Lingna Mao ^{a, d} ,
Kezhi Jiang ^{c, *,}

a.
Cancer Institute (Key Laboratory of Cancer Prevention and Intervention, China National Ministry of Education), The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310009, China
b.
Cancer Center, Zhejiang University, Hangzhou 310058, China
c.
Key Laboratory of Organosilicon Chemistry and Material Technology, College of Material Chemistry and Chemical Engineering, Hangzhou Normal University, Hangzhou 311121, China
d.
Department of General Practice, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310009, China

cheng_guo@zju.edu.cn

jiangkezhi@hznu.edu.cn

Received Date: 3 April 2023
Revised Date: 4 July 2023
Accepted Date: 27 July 2023
Available Online: 30 July 2023

Figures(5)

RNA modifications have been involved in numerous biological processes, and aberrations of these modifications are tightly associated with various diseases including cancer. Herein, we developed graphene-based solid-phase extraction and robust ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) combined with stable isotope-dilution for simultaneous enrichment and accurate determination of 17 modified nucleosides in human urine. We found graphene could effectively adsorb various modified nucleosides in human urine samples. With this method, we identified and quantified these modified nucleosides in urine samples collected from lung cancer patients and healthy controls. We revealed that the levels of 12 modified nucleosides were all diminished in urine from lung cancer patients, compared with healthy controls. It is worth noting that we demonstrated, for the first time, the presence of 5,2′-O-dimethyluridine (m⁵U_m) in human urine. Together, we established a robust analytical method for simultaneous determinations of 17 modified nucleosides in human urine, and our results revealed a close correlation between the concentrations of urinary modified nucleosides and the occurrence of lung cancer, implying the potential applications of these modified nucleosides as noninvasive biomarkers for the early detection of lung cancer. Moreover, this study will stimulate future investigations on the regulatory roles of RNA modifications in the initiation and progression of lung cancer.
- Mass spectrometry,
- RNA modification,
- Modified nucleosides,
- Graphene,
- Biomarker,
- Urine
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