Citation: Lu Zheng, Wei Cao, Biao Dou, Xueke Zeng, Mingya Cao, Jiajia Wang, Xia Li. Ac₃6deoGlcNAz could selectively label O-GlcNAc modified proteins with minimal S-glyco-modification[J]. Chinese Chemical Letters, ;2023, 34(2): 107598. doi: 10.1016/j.cclet.2022.06.021 shu

Ac₃6deoGlcNAz could selectively label O-GlcNAc modified proteins with minimal S-glyco-modification

Joint National Laboratory for Antibody Drug Engineering, The First Affiliated Hospital of Henan University, Henan University, Kaifeng 475000, China

jwang77@vip.henu.edu.cn

lixia_kf@126.com

Received Date: 11 April 2022
Revised Date: 3 June 2022
Accepted Date: 8 June 2022
Available Online: 13 June 2022

Figures(8)

A novel metabolic chemical reporter of Ac₃6deoGlcNAz was developed and confirmed as an effective probe for O-GlcNAc modification. Ac₃6deoGlcNAz labeling predominantly occurs in intracellular O-GlcNAcylated proteins rather than cell-surface glycoproteins. Of note, it could reduce the artificial S-glyco-modification compared to Ac₄GalNAz and Ac₄GlcNAz. This new reporter allows to be widely used in the field of proteomic identification of O-GlcNAcylation.
- O-GlcNAcylation,
- S-Glyco-modification,
- Metabolic labeling,
- 6-Deoxy-GlcNAz,
- Probe
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沈阳化工大学材料科学与工程学院沈阳 110142

Ac36deoGlcNAz could selectively label O-GlcNAc modified proteins with minimal S-glyco-modification

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通讯作者: 陈斌, bchen63@163.com

Ac₃6deoGlcNAz could selectively label O-GlcNAc modified proteins with minimal S-glyco-modification