Citation: Lu Zheng, Wei Cao, Biao Dou, Xueke Zeng, Mingya Cao, Jiajia Wang, Xia Li. Ac₃6deoGlcNAz could selectively label O-GlcNAc modified proteins with minimal S-glyco-modification[J]. Chinese Chemical Letters, ;2023, 34(2): 107598. doi: 10.1016/j.cclet.2022.06.021 shu

Ac₃6deoGlcNAz could selectively label O-GlcNAc modified proteins with minimal S-glyco-modification

Joint National Laboratory for Antibody Drug Engineering, The First Affiliated Hospital of Henan University, Henan University, Kaifeng 475000, China

jwang77@vip.henu.edu.cn

lixia_kf@126.com

Received Date: 11 April 2022
Revised Date: 3 June 2022
Accepted Date: 8 June 2022
Available Online: 13 June 2022

Figures(8)

A novel metabolic chemical reporter of Ac₃6deoGlcNAz was developed and confirmed as an effective probe for O-GlcNAc modification. Ac₃6deoGlcNAz labeling predominantly occurs in intracellular O-GlcNAcylated proteins rather than cell-surface glycoproteins. Of note, it could reduce the artificial S-glyco-modification compared to Ac₄GalNAz and Ac₄GlcNAz. This new reporter allows to be widely used in the field of proteomic identification of O-GlcNAcylation.
- O-GlcNAcylation,
- S-Glyco-modification,
- Metabolic labeling,
- 6-Deoxy-GlcNAz,
- Probe
1. [1]
  J. Ma, C. Wu, G.W. Hart, Chem. Rev.121 (2021) 1513-1581. doi: 10.1021/acs.chemrev.0c00884
2. [2]
  R.S. Haltiwanger, G.D. Holt, G.W. Hart, J. Biol. Chem. 265 (1990) 2563-2568. doi: 10.1016/S0021-9258(19)39838-2
3. [3]
  J.A. Hanover, S. Yu, W.B. Lubas, et al., Arch. Biochem. Biophys. 409 (2003) 287-297. doi: 10.1016/S0003-9861(02)00578-7
4. [4]
  D.L. Dong, G.W. Hart, J. Biol. Chem. 269 (1994) 19321-19330. doi: 10.1016/S0021-9258(17)32170-1
5. [5]
  Y. Wang, J. Liu, X. Jin, et al., Proc. Natl. Acad. Sci. U. S. A. 114 (2017) 13732-13737. doi: 10.1073/pnas.1704145115
6. [6]
  V. Fisi, E. Katai, J. Orban, S. Dossena, A. Miseta, T. Nagy, Molecules 23 (2018) 1275. doi: 10.3390/molecules23061275
7. [7]
  H. Nie, H. Ju, J. Fan, et al., Nat. Commun. 11 (2020) 36. doi: 10.1038/s41467-019-13601-8
8. [8]
  Q. Zhu, X. Cheng, Y. Cheng, et al., Proc. Natl. Acad. Sci. U. S. A. 117 (2020) 7755-7763. doi: 10.1073/pnas.1915582117
9. [9]
  Y. Tian, Q. Zhu, Z. Sun, et al., Angew. Chem. Int. Ed. 60 (2021) 26128-26135. doi: 10.1002/anie.202110053
10. [10]
  P.S. Banerjee, O. Lagerlof, G.W. Hart, Mol. Aspects Med. 51 (2016) 1-15. doi: 10.1016/j.mam.2016.05.005
11. [11]
  J.A. Hanover, W. Chen, M.R. Bond, J. Bioenerg. Biomembr. 50 (2018) 155-173. doi: 10.1007/s10863-018-9751-2
12. [12]
  Y. Chen, X. Zhao, H. Wu, Arterioscler. Thromb. Vasc. Biol. 39 (2019) 1911-1924. doi: 10.1161/atvbaha.119.312192
13. [13]
  W.B. Dias, G.W. Hart, Mol. Biosyst. 3 (2007) 766-772. doi: 10.1039/b704905f
14. [14]
  S. Cecioni, D.J. Vocadlo, Curr. Opin. Chem. Biol. 17 (2013) 719-28. doi: 10.1016/j.cbpa.2013.06.030
15. [15]
  M. Boyce, I.S. Carrico, A.S. Ganguli, et al., Proc. Natl. Acad. Sci. U. S. A. 108 (2011) 3141-3146. doi: 10.1073/pnas.1010045108
16. [16]
  J. Li, J. Wang, L. Wen, et al., ACS Chem. Biol. 11 (2016) 3002-3006. doi: 10.1021/acschembio.6b00678
17. [17]
  K.N. Chuh, B.W. Zaro, F. Piller, J. Am. Chem. Soc. 136 (2014) 12283-95. doi: 10.1021/ja504063c
18. [18]
  S. Li, J. Wang, L. Zang, et al., Front. Chem. 6 (2018) 646.
19. [19]
  L. Cai, W. Guan, M. Kitaoka, et al., Chem. Commun. (20) (2009) 2944-1946. doi: 10.1039/b904853g
20. [20]
  W. Qin, K. Qin, X. Fan, et al., Angew. Chem. Int. Ed. 57 (2018) 1817-1820. doi: 10.1002/anie.201711710
21. [21]
  Y. Hao, X.Q. Fan, Y.J. Shi, et al., Nat. Commun. 10 (2019) 4065. doi: 10.1038/s41467-019-11942-y
22. [22]
  J.F. Alfaro, C.X. Gong, M.E. Monroe, J.T. Aldrich, T.R. Clauss, Proc. Natl. Acad. Sci. U. S. A. 109 (2012) 7280-7285. doi: 10.1073/pnas.1200425109
23. [23]
  Z.H. Wang, N.D. Udeshi, C. Slawson, et al., Sci. Signal. 3 (2010) ra2.
1. [1]
  Yang Feng , Yang-Qing Tian , Yong-Qiang Zhao , Sheng-Jun Chen , Bi-Feng Yuan . Dynamic deformylation of 5-formylcytosine and decarboxylation of 5-carboxylcytosine during differentiation of mouse embryonic stem cells into mouse neurons. Chinese Chemical Letters, 2024, 35(11): 109656-. doi: 10.1016/j.cclet.2024.109656
2. [2]
  Hui-Juan Wang , Wen-Wen Xing , Zhen-Hai Yu , Yong-Xue Li , Heng-Yi Zhang , Qilin Yu , Hongjie Zhu , Yao-Yao Wang , Yu Liu . Cucurbit[7]uril confined phenothiazine bridged bis(bromophenyl pyridine) activated NIR luminescence for lysosome imaging. Chinese Chemical Letters, 2024, 35(6): 109183-. doi: 10.1016/j.cclet.2023.109183
3. [3]
  Guangying Wang , Qinglong Qiao , Wenhao Jia , Yiyan Ruan , Kai An , Wenchao Jiang , Xuelian Zhou , Zhaochao Xu . Adaptive emission profile of transformable fluorescent probes as fingerprints: A typical application in distinguishing different surfactants. Chinese Chemical Letters, 2025, 36(5): 110130-. doi: 10.1016/j.cclet.2024.110130
4. [4]
  Dandan Tang , Ningge Xu , Yuyang Fu , Wei Peng , Jinsheng Wu , Heng Liu , Fabiao Yu . Rationally designed an innovative proximity labeling near-infrared fluorogenic probe for imaging of peroxynitrite in acute lung injury. Chinese Chemical Letters, 2025, 36(5): 110082-. doi: 10.1016/j.cclet.2024.110082
5. [5]
  Fukui Shen , Yuqing Zhang , Guoqing Luan , Kaixue Zhang , Zhenzhen Wang , Yunhao Luo , Yuanyuan Hou , Gang Bai . Revealing drug targets with multimodal bioorthogonal AMPD probes through visual metabolic labeling. Chinese Chemical Letters, 2024, 35(12): 109646-. doi: 10.1016/j.cclet.2024.109646
6. [6]
  Tao Liu , Xuwei Han , Xueyi Sun , Weijie Zhang , Ke Gao , Runan Min , Yuting Tian , Caixia Yin . An activated fluorescent probe to monitor NO fluctuation in Parkinson’s disease. Chinese Chemical Letters, 2025, 36(3): 110170-. doi: 10.1016/j.cclet.2024.110170
7. [7]
  Shuwen SUN , Gaofeng WANG . Design and synthesis of a Zn(Ⅱ)-based coordination polymer as a fluorescent probe for trace monitoring 2, 4, 6-trinitrophenol. Chinese Journal of Inorganic Chemistry, 2025, 41(4): 753-760. doi: 10.11862/CJIC.20240399
8. [8]
  Bin Fang , Jiaqi Yang , Limin Wang , Haoqin Li , Jiaying Guo , Jiaxin Zhang , Qingyuan Guo , Bo Peng , Kedi Liu , Miaomiao Xi , Hua Bai , Li Fu , Lin Li . A mitochondria-targeted H₂S-activatable fluorogenic probe for tracking hepatic ischemia-reperfusion injury. Chinese Chemical Letters, 2024, 35(6): 108913-. doi: 10.1016/j.cclet.2023.108913
9. [9]
  Yudi Cheng , Xiao Wang , Jiao Chen , Zihan Zhang , Jiadong Ou , Mengyao She , Fulin Chen , Jianli Li . A near-infrared fluorescent probe for visualizing transformation pathway of Cys/Hcy and H₂S and its applications in living system. Chinese Chemical Letters, 2024, 35(5): 109156-. doi: 10.1016/j.cclet.2023.109156
10. [10]
  Shangqian Zhang , Jiaxuan Li , Xuan Hu , Zelong Chen , Junliang Dong , Chenhao Hu , Shuang Chao , Yinghua Lv , Yuxin Pei , Zhichao Pei . H₂S and NIR light-driven nanomotors induce disulfidptosis for targeted anticancer therapy by enhancing disruption of tumor metabolic symbiosis. Chinese Chemical Letters, 2025, 36(1): 110314-. doi: 10.1016/j.cclet.2024.110314
11. [11]
  Jie ZHANG , Xin LIU , Zhixin LI , Yuting PEI , Yuqi YANG , Huimin LI , Zhiqiang LIU . Assembling a luminescence silencing system based on post-synthetic modification strategy: A highly sensitive and selective turn-on metal-organic framework probe for ascorbic acid detection. Chinese Journal of Inorganic Chemistry, 2024, 40(4): 823-833. doi: 10.11862/CJIC.20230310
12. [12]
  Jing Guo , Zhi-Guo Lu , Rui-Chen Zhao , Bao-Ku Li , Xin Zhang . Nucleic acid therapy for metabolic-related diseases. Chinese Chemical Letters, 2025, 36(3): 109875-. doi: 10.1016/j.cclet.2024.109875
13. [13]
  Gongcheng Ma , Qihang Ding , Yuding Zhang , Yue Wang , Jingjing Xiang , Mingle Li , Qi Zhao , Saipeng Huang , Ping Gong , Jong Seung Kim . Palladium-free chemoselective probe for in vivo fluorescence imaging of carbon monoxide. Chinese Chemical Letters, 2024, 35(9): 109293-. doi: 10.1016/j.cclet.2023.109293
14. [14]
  Chuan-Zhi Ni , Ruo-Ming Li , Fang-Qi Zhang , Qu-Ao-Wei Li , Yuan-Yuan Zhu , Jie Zeng , Shuang-Xi Gu . A chiral fluorescent probe for molecular recognition of basic amino acids in solutions and cells. Chinese Chemical Letters, 2024, 35(10): 109862-. doi: 10.1016/j.cclet.2024.109862
15. [15]
  Zhihui Zhang , Ru Sun , Chong Bian , Hongbo Wang , Zhen Zhao , Panpan Lv , Jianzhong Lu , Haixin Zhang , Hulie Zeng , Yuanyuan Chen , Zhijuan Cao . A dual-protease-triggered chemiluminescent probe for precise tumor imaging. Chinese Chemical Letters, 2025, 36(2): 109784-. doi: 10.1016/j.cclet.2024.109784
16. [16]
  Qianqian Liu , Xing Du , Wanfei Li , Wei-Lin Dai , Bo Liu . Synergistic Effects of Internal Electric and Dipole Fields in SnNb₂O₆/Nitrogen-Enriched C₃N₅ S-Scheme Heterojunction for Boosting Photocatalytic Performance. Acta Physico-Chimica Sinica, 2024, 40(10): 2311016-. doi: 10.3866/PKU.WHXB202311016
17. [17]
  Xing Tian , Di Wu , Wanheng Wei , Guifu Dai , Zhanxian Li , Benhua Wang , Mingming Yu . A lipid droplets-targetable fluorescent probe for polarity detection in cells of iron death, inflammation and fatty liver tissue. Chinese Chemical Letters, 2024, 35(6): 108912-. doi: 10.1016/j.cclet.2023.108912
18. [18]
  Linfang Wang , Jing Liu , Minghao Ren , Wei Guo . A highly sensitive fluorescent HClO probe for discrimination between cancerous and normal cells/tissues. Chinese Chemical Letters, 2024, 35(6): 108945-. doi: 10.1016/j.cclet.2023.108945
19. [19]
  Yang Liu , Leilei Zhang , Kaixuan Liu , Ling-Ling Wu , Hai-Yu Hu . Penicillin G acylase-responsive near-infrared fluorescent probe: Unravelling biofilm regulation and combating bacterial infections. Chinese Chemical Letters, 2024, 35(11): 109759-. doi: 10.1016/j.cclet.2024.109759
20. [20]
  Huamei Zhang , Jingjing Liu , Mingyue Li , Shida Ma , Xucong Zhou , Aixia Meng , Weina Han , Jin Zhou . Imaging polarity changes in pneumonia and lung cancer using a lipid droplet-targeted near-infrared fluorescent probe. Chinese Chemical Letters, 2024, 35(12): 110020-. doi: 10.1016/j.cclet.2024.110020

Get Citation

PDF

XML

Metrics

PDF Downloads(0)
Abstract views(678)
HTML views(52)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Ac36deoGlcNAz could selectively label O-GlcNAc modified proteins with minimal S-glyco-modification

Metrics

通讯作者: 陈斌, bchen63@163.com

Ac₃6deoGlcNAz could selectively label O-GlcNAc modified proteins with minimal S-glyco-modification