Citation: Lijuan Gui, Kaizhen Wang, Yuxin Wang, Jun Yan, Xian Liu, Jingxuan Guo, Ji Liu, Dawei Deng, Haiyan Chen, Zhenwei Yuan. Monitoring the pH fluctuation of lysosome under cell stress using a near-infrared ratiometric fluorescent probe[J]. Chinese Chemical Letters, ;2023, 34(3): 107586. doi: 10.1016/j.cclet.2022.06.009 shu

Monitoring the pH fluctuation of lysosome under cell stress using a near-infrared ratiometric fluorescent probe

Lijuan Gui ^{a, 1} ,
Kaizhen Wang ^{a, 1} ,
Yuxin Wang ^a ,
Jun Yan ^a ,
Xian Liu ^b ,
Jingxuan Guo ^a ,
Ji Liu ^a ,
Dawei Deng ^{a, *,} ,
Haiyan Chen ^{a, *,} ,
Zhenwei Yuan ^{a, *,}

a.
Department of Biomedical Engineering, School of Engineering, China Pharmaceutical University, Nanjing 210009, China
b.
Department of Health Technology and Informatics, Faculty of Health and Social Sciences, The Hong Kong Polytechnic University, Hong Kong, China

dengdawei@cpu.edu.cn

chenhaiyan@cpu.edu.cn

yuanzhenwei@cpu.edu.cn

Received Date: 14 March 2022
Revised Date: 1 June 2022
Accepted Date: 5 June 2022
Available Online: 10 June 2022

Figures(6)

Cell stress responses are associated with numerous diseases including diabetes, neurodegenerative diseases, and cancer. Several events occur under cell stress, in which, are protein expression and organelle-specific pH fluctuation. To understand the lysosomal pH variation under cell stress, a novel NIR ratiometric pH-responsive fluorescent probe (BLT) with lysosomes localization capability was developed. The quinoline ring of BLT combined with hydrogen ion which triggered the rearrangement of π electrons conjugated at low pH medium, meanwhile, the absorption and fluorescent spectra of BLT showed a red-shifts, which gived a ratiometric signal. Moreover, the probe BLT with a suitable pK_a value has the potential to discern changes in lysosomal pH, either induced by heat stress or oxidative stress or acetaminophen-induced (APAP) injury stress. Importantly, this ratiometric fluorescent probe innovatively tracks pH changes in lysosome in APAP-induced liver injury in live cells, mice, and zebrafish. The probe BLT as a novel fluorescent probe possesses important value for exploring lysosomal-associated physiological varieties of drug-induced hepatotoxicity.
- APAP-induced liver injury,
- Ratiometric fluorescent probe,
- Lysosomal pH,
- Cell stress
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