Citation: Guobin Han, Dandan Li, Qiuyuan Lin, Jia Yi, Qian Lyu, Qingwei Ma, Liang Qiao. Exponential isothermal amplification coupled MALDI-TOF MS for microRNAs detection[J]. Chinese Chemical Letters, ;2023, 34(2): 107421. doi: 10.1016/j.cclet.2022.04.019 shu

Exponential isothermal amplification coupled MALDI-TOF MS for microRNAs detection

Guobin Han ^a ,
Dandan Li ^a ,
Qiuyuan Lin ^a ,
Jia Yi ^a ,
Qian Lyu ^b ,
Qingwei Ma ^b ,
Liang Qiao ^{a, *,}

a.
Department of Chemistry, Shanghai Stomatological Hospital, Fudan University, Shanghai 200000, China
b.
Bioyong Technologics, Inc., Beijing 100176, China

liang_qiao@fudan.edu.cn

Received Date: 12 January 2022
Revised Date: 20 February 2022
Accepted Date: 8 April 2022
Available Online: 14 April 2022

Figures(4)

MicroRNAs (miRNAs) have attracted significant attention in biomedical research and clinical diagnosis. However, due to their inherent characteristics of low abundance and the high complexity of corresponding biological matrices, simultaneous detection of multiple miRNAs at low abundance is still a challenge. In this work, a method coupling exponential amplification reaction (EXPAR) with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is developed for label-free and simultaneous detection of multiple miRNAs. The assay can be performed under isothermal conditions in a single reaction tube, and finished in less than 30 min. It exhibits good quantification ability and with attomolar-level sensitivity for miRNAs detection. It also shows high specificity to distinguish miRNAs at single-nucleotide resolution. We used the method to detect the miRNA-21, let-7a, miRNA-100, and miRNA-125b in samples of spiked human serum and breast cancer cells (i.e., MCF-7, MDA-MB-231 and SK-BR-3). The quantification results were well consistent with the standard real-time fluorescence EXPAR. Consequently, the label-free mass-spectrometric platform could be a potential tool for miRNAs analysis in complex biological samples, and may be used for clinical diagnosis.
- MicroRNA,
- EXPAR,
- Isothermal amplification,
- MALDI-TOF MS,
- Breast cancer cells
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