Citation: Guobin Han, Dandan Li, Qiuyuan Lin, Jia Yi, Qian Lyu, Qingwei Ma, Liang Qiao. Exponential isothermal amplification coupled MALDI-TOF MS for microRNAs detection[J]. Chinese Chemical Letters, ;2023, 34(2): 107421. doi: 10.1016/j.cclet.2022.04.019 shu

Exponential isothermal amplification coupled MALDI-TOF MS for microRNAs detection

Guobin Han ^a ,
Dandan Li ^a ,
Qiuyuan Lin ^a ,
Jia Yi ^a ,
Qian Lyu ^b ,
Qingwei Ma ^b ,
Liang Qiao ^{a, *,}

a.
Department of Chemistry, Shanghai Stomatological Hospital, Fudan University, Shanghai 200000, China
b.
Bioyong Technologics, Inc., Beijing 100176, China

liang_qiao@fudan.edu.cn

Received Date: 12 January 2022
Revised Date: 20 February 2022
Accepted Date: 8 April 2022
Available Online: 14 April 2022

Figures(4)

MicroRNAs (miRNAs) have attracted significant attention in biomedical research and clinical diagnosis. However, due to their inherent characteristics of low abundance and the high complexity of corresponding biological matrices, simultaneous detection of multiple miRNAs at low abundance is still a challenge. In this work, a method coupling exponential amplification reaction (EXPAR) with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is developed for label-free and simultaneous detection of multiple miRNAs. The assay can be performed under isothermal conditions in a single reaction tube, and finished in less than 30 min. It exhibits good quantification ability and with attomolar-level sensitivity for miRNAs detection. It also shows high specificity to distinguish miRNAs at single-nucleotide resolution. We used the method to detect the miRNA-21, let-7a, miRNA-100, and miRNA-125b in samples of spiked human serum and breast cancer cells (i.e., MCF-7, MDA-MB-231 and SK-BR-3). The quantification results were well consistent with the standard real-time fluorescence EXPAR. Consequently, the label-free mass-spectrometric platform could be a potential tool for miRNAs analysis in complex biological samples, and may be used for clinical diagnosis.
- MicroRNA,
- EXPAR,
- Isothermal amplification,
- MALDI-TOF MS,
- Breast cancer cells
1. [1]
  D.P. Bartel, Cell 116 (2004) 281-297. doi: 10.1016/S0092-8674(04)00045-5
2. [2]
  H. Dong, J. Lei, L. Ding, et al., Chem. Rev. 113 (2013) 6207-6233. doi: 10.1021/cr300362f
3. [3]
  D.P. Bartel, Cell 136 (2009) 215-233. doi: 10.1016/j.cell.2009.01.002
4. [4]
  J. Ko, N. Bhagwat, T. Black, et al., Cancer Res. 78 (2018) 3688-3697. doi: 10.1158/0008-5472.can-17-3703
5. [5]
  J.S. Nahand, S. Taghizadeh-Boroujeni, M. Karimzadeh, et al., J. Cell Physiol. 234 (2019) 17064-17099. doi: 10.1002/jcp.28457
6. [6]
  M. Yi, L. Xu, Y. Jiao, et al., J. Hematol. Oncol. 13 (2020) 25. doi: 10.1186/s13045-020-00848-8
7. [7]
  S. Babashah, M. Soleimani, Eur. J. Cancer 47 (2011) 1127-1137. doi: 10.1016/j.ejca.2011.02.008
8. [8]
  M.A. Mori, R.G. Ludwig, R. Garcia-Martin, et al., Cell Metab. 30 (2019) 656-673. doi: 10.1016/j.cmet.2019.07.011
9. [9]
  W. Zhang, Z. Han, Y. Liang, et al., Chin. Chem. Lett. 32 (2021) 2183-2186. doi: 10.1016/j.cclet.2020.12.007
10. [10]
  M. Schwarzkopf, N.A. Pierce, Nucleic Acids Res. 44 (2016) e129.
11. [11]
  J.M. Thomson, J. Parker, C.M. Perou, S.M. Hammond, Nat. Methods 1 (2004) 47-53. doi: 10.1038/nmeth704
12. [12]
  M. Xu, J. Ye, D. Yang, et al., Anal. Chim. Acta 1077 (2019) 208-215. doi: 10.1016/j.aca.2019.05.028
13. [13]
  T. Ouyang, Z. Liu, Z. Han, Q. Ge, Anal. Chem. 91 (2019) 3179-3186. doi: 10.1021/acs.analchem.8b05909
14. [14]
  J. Chen, T. Jin, J. Li, et al., ACS Appl. Bio Mater. 4 (2021) 820-828. doi: 10.1021/acsabm.0c01362
15. [15]
  F. Gao, Y. Chu, Y. Ai, et al., Chin. Chem. Lett. 32 (2021) 2192-2196. doi: 10.1016/j.cclet.2020.12.036
16. [16]
  X. Feng, N. Gan, H. Zhang, et al., Biosens. Bioelectron. 75 (2016) 308-314. doi: 10.1016/j.bios.2015.08.048
17. [17]
  Y. Zhao, X. Fang, M. Bai, et al., Chin. Chem. Lett. 33 (2022) 2101–2104. doi: 10.1016/j.cclet.2021.08.047
18. [18]
  C. Song, W. Chen, J. Kuang, et al., TrAC-Trends Anal. Chem. 139 (2021) 116269. doi: 10.1016/j.trac.2021.116269
19. [19]
  T.C. de Bang, P. Shah, S.K. Cho, et al., Anal. Chem. 86 (2014) 6823-6826. doi: 10.1021/ac5017166
20. [20]
  S. Zhang, R. Liub, Z. Xing, et al., Chem. Commun. 52 (2016) 14310-14313. doi: 10.1039/C6CC08334J
21. [21]
  C.X. Shi, S.X. Li, Z.P. Chen, et al., Anal. Chem. 91 (2019) 2120-2127. doi: 10.1021/acs.analchem.8b04583
22. [22]
  H. Köster, K. Tang, D.J. Fu, et al., Nat. Biotechnol. 14 (1996) 1123-1128. doi: 10.1038/nbt0996-1123
23. [23]
  U. Puapaiboon, J. Jai-nhuknan, J.A. Cowan, Anal. Chem. 72 (2000) 3338-3341. doi: 10.1021/ac000181n
24. [24]
  H. Su, X. Li, L. Huang, et al., Adv. Mater. 33 (2021) e2007978. doi: 10.1002/adma.202007978
25. [25]
  L. Huang, L. Wang, X. Hu, et al., Nat. Commun. 11 (2020) 3556. doi: 10.1038/s41467-020-17347-6
26. [26]
  D.M. Wambua, M. Ubukata, J. Dane, et al., Anal. Methods 6 (2014) 8829-8839. doi: 10.1039/C4AY01519C
27. [27]
  C. Pei, C. Liu, Y. Wang, et al., Angew. Chem. Int. Ed. 59 (2020) 10831-10835. doi: 10.1002/anie.202001135
28. [28]
  L.Z. Samarah, A. Vertes, VIEW 1 (2020) 20200063. doi: 10.1002/viw.20200063
29. [29]
  M.B. Lambros, P.M. Wilkerson, R. Natrajan, et al., Lab. Invest. 91 (2011) 1491-1501. doi: 10.1038/labinvest.2011.110
30. [30]
  Y. Belloum, M. Janning, M. Mohme, et al., Cells 9 (2020) 2337. doi: 10.3390/cells9112337
31. [31]
  S. Sauer, R. Reinhardt, H. Lehrach, I.G. Gut, Nat. Protoc. 1 (2006) 1761-1771. doi: 10.1038/nprot.2006.257
32. [32]
  R. Claus, S. Wilop, T. Hielscher, et al., Epigenetics 7 (2012) 772-780. doi: 10.4161/epi.20299
33. [33]
  X.W. Xing, F. Tang, J. Wu, et al., Anal. Chem. 86 (2014) 11269-11274. doi: 10.1021/ac502845b
34. [34]
  Y. Zhao, F. Chen, Q. Li, et al., Chem. Rev. 115 (2015) 12491-12545. doi: 10.1021/acs.chemrev.5b00428
35. [35]
  M.S. Reid, X.C. Le, H. Zhang, Angew. Chem. Int. Ed. 57 (2018) 11856-11866. doi: 10.1002/anie.201712217
36. [36]
  K. Liu, C. Zhang, T. Li, et al., Int. J. Oncol. 46 (2015) 2526-2534. doi: 10.3892/ijo.2015.2949
37. [37]
  M.A. Tfaily, F. Nassar, L.S. Sellam, et al., PLoS One 15 (2020) e0227928. doi: 10.1371/journal.pone.0227928
38. [38]
  S.L. Cohen, B.T. Chait, Anal. Chem. 68 (1996) 31-37. doi: 10.1021/ac9507956
39. [39]
  H. Jia, Z. Li, C. Liu, Y. Cheng, Angew. Chem. Int. Ed. 49 (2010) 5498-5501. doi: 10.1002/anie.201001375
40. [40]
  W. Zhou, Y.F. Tian, B.C. Yin, B.C. Ye, Anal. Chem. 89 (2017) 6120-6128. doi: 10.1021/acs.analchem.7b00902
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