Citation: Wuzhen Qi, Siyuan Wang, Lei Wang, Xinge Xi, Shangyi Wu, Yanbin Li, Ming Liao, Jianhan Lin. A portable viable Salmonella detection device based on microfluidic chip and recombinase aided amplification[J]. Chinese Chemical Letters, ;2023, 34(2): 107360. doi: 10.1016/j.cclet.2022.03.083 shu

A portable viable Salmonella detection device based on microfluidic chip and recombinase aided amplification

Wuzhen Qi ^a ,
Siyuan Wang ^a ,
Lei Wang ^a ,
Xinge Xi ^a ,
Shangyi Wu ^a ,
Yanbin Li ^b ,
Ming Liao ^c ,
Jianhan Lin ^{a, *,}

a.
Key Laboratory of Agricultural Information Acquisition Technology, Ministry of Agriculture and Rural Affairs, China Agricultural University, Beijing 100083, China
b.
Department of Biological and Agricultural Engineering, University of Arkansas, Fayetteville 72701, United States
c.
College of Veterinary medicine, South China Agricultural University, Guangzhou 510642, China

jianhan@cau.edu.cn

Received Date: 3 December 2021
Revised Date: 12 February 2022
Accepted Date: 21 March 2022
Available Online: 23 March 2022

Figures(4)

Screening of foodborne pathogens is important to prevent contaminated foods from their supply chains. In this study, a portable detection device was developed for rapid, sensitive and simple detection of viable Salmonella using a finger-actuated microfluidic chip and an improved recombinase aided amplification (RAA) assay. Improved propidium monoazide (PMAxx) was combined with RAA to enable this device to distinguish viable bacteria from dead ones. The modification of PMAxx into dead bacteria, the magnetic extraction of nucleic acids from viable bacteria and the RAA detection of extracted nucleic acids were performed using the microfluidic chip on its supporting device by finger press-release operations. The fluorescent signal resulting from RAA amplification of the nucleic acids was collected using a USB camera and analyzed using a self-developed smartphone App to quantitatively determine the bacterial concentration. This device could detect Salmonella typhimurium in spiked chicken meats from 1.3 × 10² CFU/mL to 1.3 × 10⁷ CFU/mL in 2 h with a lower detection limit of 130 CFU/mL, and has shown its potential for on-site detection of foodborne pathogens.
- Finger-actuated microfluidic chip,
- PMAxx,
- Recombinase aid amplification,
- Smartphone App,
- Viable bacterial detection
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