Citation: Manping Qian, Danyang Zhang, Hetong Qi, Xuebing Yang, Guanjun Yin, Chengxiao Zhang, Jianzhong Guo, Honglan Qi. pH-responsive aldehyde-bearing cyclometalated iridium(Ⅲ) complex for tracking intracellular pH fluctuations under external stimulation[J]. Chinese Chemical Letters, ;2023, 34(2): 107336. doi: 10.1016/j.cclet.2022.03.059 shu

pH-responsive aldehyde-bearing cyclometalated iridium(Ⅲ) complex for tracking intracellular pH fluctuations under external stimulation

a.
Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an 710062, China
b.
School of Chemistry, Xi'an Jiaotong University, Xi'an 710049, China
c.
Shaanxi Province Key Laboratory of Ultrasound, School of Physics and Information Technology, Shaanxi Normal University, Xi'an 710062, China

honglanqi@snnu.edu.cn

Received Date: 29 December 2021
Revised Date: 8 February 2022
Accepted Date: 14 March 2022
Available Online: 18 March 2022

Figures(6)

Intracellular pH undertakes critical functions in various biological and pathological processes. It is important to monitor intracellular pH fluctuations for understanding physiological and pathological processes. Here, one aldehyde-bearing cyclometalated iridium(Ⅲ) complex ([(4-pba)₂Ir(dcphen)]PF₆, 4-pba = 4-(2-pyridyl) benzaldehyde, dcphen = 4, 7-dichloro-1, 10-phenanthroline, probe 1) was synthesized and used to track intracellular pH fluctuations. Probe 1 displayed pH-dependent luminescence property in pH range of 1.81–6.81 with an evaluated pK_a value of 4.30 in BR buffer-DMSO (v:v = 99:1). An intramolecular hydrogen bonds assisted pH-responsive mechanism was proposed for the pH-responsive behavior of probe 1. Probe 1 was successfully applied for imaging and tracking pH fluctuations in HeLa cells under external stimulation with fast response time, good photostability as well as low cytotoxicity and high cell permeability. This work demonstrates that aldehyde-bearing cyclometalated iridium(Ⅲ) complex can be used as alternative pH-responsive probe for real-time tracking intracellular pH fluctuations, which provides a strategy for the design of pH-responsive probe in versatile applications.
- Cyclometalated iridium(Ⅲ) complex,
- Photoluminescence,
- pH,
- Intramolecular hydrogen bonds,
- External stimulation
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