Citation: Yunxing Lu, Zhaoduo Tong, Zhenhua Wu, Xiaoyu Jian, Lin Zhou, Shihui Qiu, Chuanjie Shen, Hao Yin, Hongju Mao. Multiple exosome RNA analysis methods for lung cancer diagnosis through integrated on-chip microfluidic system[J]. Chinese Chemical Letters, ;2022, 33(6): 3188-3192. doi: 10.1016/j.cclet.2021.12.045 shu

Multiple exosome RNA analysis methods for lung cancer diagnosis through integrated on-chip microfluidic system

Yunxing Lu ^{a, b} ,
Zhaoduo Tong ^{a, b} ,
Zhenhua Wu ^a ,
Xiaoyu Jian ^a ,
Lin Zhou ^a ,
Shihui Qiu ^{a, b} ,
Chuanjie Shen ^{a, b} ,
Hao Yin ^{a, b} ,
Hongju Mao ^{a, b, *}

a.
State Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai 200050, China
b.
Center of Materials Science and Optoelectronics Engineering, University of Chinese Academy of Sciences, Beijing 100049, China

hjmao@mail.sim.ac.cn

Received Date: 10 July 2021
Revised Date: 24 September 2021
Accepted Date: 20 December 2021
Available Online: 24 December 2021

Figures(6)

Exosomes are now raising focus as a prospective biomarker for cancer diagnostics and prognosis owing to its unique bio-origin and composition. Exosomes take part in cellular communication and receptor mediation and transfer their cargos (e.g., proteins, mRNA and DNA). Quantitative analysis of tumor-related nucleic acid mutations can be a potential method to cancer diagnosis and prognosis in early stages. Here we present an integrated microfluidic system for exosome on-chip isolation and lung cancer RNA analysis through droplet digital PCR (ddPCR). Gradient dilution experiments show great linearity over a large concentration range with R² = 0.9998. Utilizing the system, four cell lines and two mutation targets were parallelly detected for mutation analysis. The experiments demonstrated mutation heterogeneity and the results were agree with cell researches. These results proved our integrated microfluidic system as a promising means for early cancer diagnosis and prognosis in the era of liquid biopsy.
- Exosome,
- Integrated processing,
- RNA analysis,
- Microfluidics,
- Droplet digital PCR
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