Citation: Chuanhao Liu, Lin Zhou, Lijuan Xie, Ying Zheng, Huizi Man, Yi Xiao. Forthrightly monitoring ferroptosis induced by endoplasmic reticulum stresses through fluorescence lifetime imaging of microviscosity increases with a specific rotor[J]. Chinese Chemical Letters, ;2022, 33(5): 2537-2540. doi: 10.1016/j.cclet.2021.11.082 shu

Forthrightly monitoring ferroptosis induced by endoplasmic reticulum stresses through fluorescence lifetime imaging of microviscosity increases with a specific rotor

Chuanhao Liu ^{a, b} ,
Lin Zhou ^b ,
Lijuan Xie ^{a, *,} ,
Ying Zheng ^b ,
Huizi Man ^b ,
Yi Xiao ^{b, *,}

a.
Engineering Research Center of Molecular Medicine of Ministry of Education, Key Laboratory of Fujian Molecular Medicine, School of Medicine, Huaqiao University, Quanzhou 362021, China
b.
State Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian 116024, China

xielijuan@hqu.edu.cn

xiaoyi@dlut.edu.cn

Received Date: 17 August 2021
Revised Date: 23 November 2021
Accepted Date: 26 November 2021
Available Online: 1 December 2021
Fund Project: the National Natural Science Foundation of China 21776037the National Natural Science Foundation of China 22174009Dalian Science and Technology Innovation Fund 2020JJ25CY014Quanzhou Science and Technology Plan Project 2019C033R

Figures(6)

To test the hypothesis that the microviscosity changes of Endoplasmic Reticulum (ER) can be a useful indicator of ferroptosis promoted by ER Stresses (ERS), a new ER targeting viscosity rotor, L-Vis-1 was developed and applied in the quantitation of viscosity by FLIM imaging in live cells. The FLIM imaging exhibited an excellent resolution almost as good as the corresponding confocal imaging, more significantly, during ferroptosis processes promoted by different types of ERS, the viscosity increases were clearly monitored by FLIM of L-Vis-1 within ER, which has not been demonstrated before.
- Ferroptosis,
- Viscosity,
- FLIM,
- ERS,
- Rotor
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