Citation: Huimin Chen, Han Wang, Yongchun Wei, Maomao Hu, Bo Dong, Hongbao Fang, Qixin Chen. Super-resolution imaging reveals the subcellular distribution of dextran at the nanoscale in living cells[J]. Chinese Chemical Letters, ;2022, 33(4): 1865-1869. doi: 10.1016/j.cclet.2021.10.025 shu

Super-resolution imaging reveals the subcellular distribution of dextran at the nanoscale in living cells

Huimin Chen ^a ,
Han Wang ^{a, b} ,
Yongchun Wei ^a ,
Maomao Hu ^{a, b} ,
Bo Dong ^b ,
Hongbao Fang ^c ,
Qixin Chen ^{a, *,}

a.
Institute of Materia Medica, Shandong First Medical University & Shandong Academy of Medical Sciences, Ji'nan 250062, China
b.
Department of Cardiology, Shandong Provincial Hospital Affiliated to Shandong University, Ji'nan 250012, China
c.
State Key Laboratory of Coordination Chemistry, Chemistry and Biomedicine Innovation Center (ChemBIC), School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, China

chenqixin@sdfmu.edu.cn

Received Date: 26 June 2021
Revised Date: 9 October 2021
Accepted Date: 11 October 2021
Available Online: 16 October 2021

Figures(4)

Theranostic visualization of dextran at the nanoscale is beneficial for understanding the bioregulatory mechanisms of this molecule. In this study, we applied structured illumination microscopy (SIM) to capture the distribution of Cy5-Dextran at different incubation periods in living cells. The results showed that Cy5-Dextran could be absorbed by HeLa cells. In addition, we clarified that Cy5-Dextran exhibited differential organelle distribution (lysosomal or mitochondrial) in a time-dependent manner. Moreover, lysosomal Cy5-Dextran localization was found to be independent of the autophagy process, while Cy5-Dextran localized to the mitochondria triggered a pro-apoptotic event, upregulating the levels of reactive oxygen species (ROS) to accelerate mitochondrial fragmentation. This work uses a visualized strategy to reveal the anti-tumor bioactivity of dextran, which was achieved by regulating apoptosis and autophagy.
- Dextran,
- Subcellular distribution,
- Super-resolution imaging,
- Structured illumination microscopy
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