Citation: Yixin Liu, Yuanyuan Yao, Beibei Yang, Yan-Jun Liu, Baohong Liu. In-situ monitoring of cell-secreted lactate by electrochemiluminescence sensing under biomimetic microfluidic confinement[J]. Chinese Chemical Letters, ;2022, 33(5): 2705-2707. doi: 10.1016/j.cclet.2021.09.074 shu

In-situ monitoring of cell-secreted lactate by electrochemiluminescence sensing under biomimetic microfluidic confinement

Yixin Liu ^a ,
Yuanyuan Yao ^c ,
Beibei Yang ^d ,
Yan-Jun Liu ^{b, *,} ,
Baohong Liu ^{a, *,}

a.
Department of Chemistry, Shanghai Stomatological Hospital, and State Key Lab of Molecular Engineering of Polymers, Fudan University, Shanghai 200433, China
b.
Shanghai Key Laboratory of Medical Epigenetics, International Co-laboratory of Medical Epigenetics and Metabolism (Ministry of Science and Technology), Institutes of Biomedical Sciences, Fudan University, Shanghai 200032, China
c.
College of Biological, Chemical Sciences and Engineering, Jiaxing University, Jiaxing 314001, China
d.
Department of Chemistry and Chemical Engineering, Nantong University, Nantong 226000, China

yanjun_liu@fudan.edu.cn

bhliu@fudan.edu.cn

Received Date: 31 May 2021
Revised Date: 6 September 2021
Accepted Date: 22 September 2021
Available Online: 29 September 2021

Figures(4)

Cell migration proceeds in 3D matrices in vivo, which can naturally switch to distinct phenotypes for better invasion in confined microenvironments. The studies of important metabolites under confinement are extremely meaningful for comprehensive insights into cancer metastasis. The integration of cell confinement device and analytical techniques is a key point for in-situ analysis of significant metabolites in vitro. Herein, an electrochemiluminescence (ECL) sensing platform was designed for in-situ monitoring of cell-secreted lactate in highly confined microenvironments. The 3-µm confiner was exactly fabricated via microfabrication and microfluidics technique, and cells in high confinement and low adhesion tended to be round with contractile blebs on cell margins. Significantly, in-situ monitoring of lactate was successfully achieved on the ECL platform with the catalysis of lactate oxidase, in which the levels in different time intervals were acquired in the luminol-hydrogen peroxide system. Furthermore, the results were verified by the liquid chromatography-tandem mass spectrometry (LC-MS/MS) technology, which showed similar fluctuations with the ECL platform. This system offered an available avenue for metabolites analysis in highly confined microenvironments, which may advance deeper insights into metabolic mechanisms of cancer metastasis
- Confinement,
- ECL sensing platform,
- In-situ monitoring
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