Citation: Li-Jun Xie, Cui-Lian Lin, Li Liu, Liang Cheng. A chemical labeling of N⁶-formyl adenosine (f⁶A) RNA[J]. Chinese Chemical Letters, ;2022, 33(3): 1563-1566. doi: 10.1016/j.cclet.2021.09.028 shu

A chemical labeling of N⁶-formyl adenosine (f⁶A) RNA

Li-Jun Xie ^a ,
Cui-Lian Lin ^{a, b} ,
Li Liu ^{a, b} ,
Liang Cheng ^{a, b, *,}

a.
Beijing National Laboratory for Molecular Sciences (BNLMS), CAS Key Laboratory of Molecular Recognition and Function, CAS Research/Education Center for Excellence in Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China
b.
University of Chinese Academy of Sciences, Beijing 100049, China

chengl@iccas.ac.cn

Received Date: 1 July 2021
Revised Date: 3 September 2021
Accepted Date: 7 September 2021
Available Online: 11 September 2021

Figures(5)

N⁶-methyl adenosine (m⁶A) is an eminent epigenetic mark in mRNAs that affects a broad range of biological functions in diverse species. However, the chemically inert methyl group prevents a direct labeling of this modification for subsequent detection and sequencing. Therefore, most current approaches for the labeling of m⁶A still have limitations of relying on the utilization of corresponding methyltransferases, which resulted in the lacking of efficiency. Here we present an approach which selectively alkylated the N⁶-formyl adenosine (f⁶A), the key intermediate during chemical oxidation of m⁶A, with an alkyne functionality that can be further labeled with click reactions. This covalent labeling approach will be able to facilitate in the affinity purification, detection and genome-wide profiling studies.
- RNA epigenetics,
- Chemical modulation,
- Labeling,
- Nucleic acids,
- Alkylation
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A chemical labeling of N⁶-formyl adenosine (f⁶A) RNA