Citation: Ruizhi Ning, Jinhai Fan, Liang Kong, Xue Jiang, Yun Qian, Tao Du, Guangjian Zhang, Weiwei Wu. Recent developments of droplets-based microfluidics for bacterial analysis[J]. Chinese Chemical Letters, ;2022, 33(5): 2243-2252. doi: 10.1016/j.cclet.2021.08.096 shu

Recent developments of droplets-based microfluidics for bacterial analysis

Ruizhi Ning ^{a, *,} ,
Jinhai Fan ^b ,
Liang Kong ^c ,
Xue Jiang ^a ,
Yun Qian ^d ,
Tao Du ^a ,
Guangjian Zhang ^{b, *,} ,
Weiwei Wu ^{a, *,}

a.
Interdisciplinary Research Center of Smart Sensors, Academy of Advanced Interdisciplinary Research, Xidian University, Xi'an 710071, China
b.
The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061, China
c.
State Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases and Shaanxi Clinical Research Center for Oral Diseases, Department of Oral and Maxillofacial Surgery, School of Stomatology, Air Force Medical University, Xi'an 710032, China
d.
Stomatology Hospital, School of Stomatology, Zhejiang University School of Medicine, Clinical Research Center for Oral Diseases of Zhejiang Province, Key Laboratory of Oral Biomedical Research of Zhejiang Province, Cancer Center of Zhejiang University, Hangzhou 310006, China

rzning@xidian.edu.cn

michael8039@163.com

wwwu@xidian.edu.cn

Received Date: 30 May 2021
Revised Date: 24 June 2021
Accepted Date: 20 August 2021
Available Online: 26 August 2021

Figures(4)

Droplet-based microfluidics enables the generation of uniform microdroplets at picoliter or nanoliter scale with high frequency (~kHz) under precise control. The droplets can function as bioreactors for versatile chemical/biological study and analysis. Taking advantage of the discrete compartment with a confined volume, (1) isolation and manipulation of a single cell, (2) improvement of in-droplet effective concentrations, (3) elimination of heterogeneous population effects, (4) diminution of contamination risks can be achieved, making it a powerful tool for rapid, sensitive, and high-throughput detection and analysis of bacteria, even for rare or unculturable strains in conventional methods. This mini-review will focus on the generation and manipulation of micro-droplets and bacteria detection and analysis carried out by droplet-based microfluidics. Finally, applications with high potential of droplet-based bacteria analysis are briefly introduced. Due to the advantages of rapid, sensitive, high throughput, and compatibility with rare and unculturable bacteria in conventional methods, droplet-based microfluidics has tremendous potential of providing novel solutions for biological medicine, microbiological engineering, environmental ecology, etc.
- Droplet microfluidics,
- Droplet generation,
- Droplet manipulation,
- High throughput,
- Bacteria analysis
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