Citation: Wei Zhou, Xiangning Fang, Qinglong Qiao, Wenchao Jiang, Yue Zhang, Zhaochao Xu. Quantitative assessment of rhodamine spectra[J]. Chinese Chemical Letters, ;2021, 32(2): 943-946. doi: 10.1016/j.cclet.2021.02.003 shu

Quantitative assessment of rhodamine spectra

Wei Zhou ^{a, b, 1} ,
Xiangning Fang ^{a, 1} ,
Qinglong Qiao ^a ,
Wenchao Jiang ^a ,
Yue Zhang ^a ,
Zhaochao Xu ^{a, b, *,}

a.
CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China
b.
Zhang Dayu School of Chemistry, Dalian University of Technology, Dalian 116012, China

zcxu@dicp.ac.cn

Received Date: 11 January 2020
Revised Date: 19 February 2020
Accepted Date: 1 February 2020
Available Online: 4 February 2020

Figures(2)

With the development of single-molecule detection and super-resolution fluorescence imaging, rhodamine dyes gain new life. Through the modification of the N-substituents and the replacement of the oxygen atom in xanthene, the wavelength and brightness can be effectively changed. However, the spectra of rhodamine, especially due to the balance between ring-closed non-fluorescent lactone and ring-opened fluorescent zwitterion/cation, are sensitive to interference from various environmental factors. In this way, the spectral data of various rhodamines reported by different research groups under different test conditions lacked comparability, sometimes even lacked accuracy. In order to meet the requirements for the accuracy and uniformity of spectral data in the research of single molecule imaging and dye structure-fluorescence relationship study, we have tested the spectra of fifteen rhodamine dyes that cover the visible and near-infrared regions under exactly the same conditions. By studying the dependence of the spectra on dye concentrations, it was confirmed that 1 μmol/L was ideal for detection less from the interference of dye molecule aggregation. We provide comprehensive and reliable spectral data of these fifteen dyes, which are expected to be used as references for future research. And the direct comparison of different rhodamine spectra would help to understand the structure-fluorescence relationship of rhodamines.
- Rhodamine,
- Quantitative assessment,
- Absorption,
- Emission,
- Quantum yield
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