Citation: Kemei Jiang, Yanan Wu, Juan Chen, Mingqing Shi, Hong-Min Meng, Zhaohui Li. Molecular recognition triggered aptazyme cascade for ultrasensitive detection of exosomes in clinical serum samples[J]. Chinese Chemical Letters, ;2021, 32(5): 1827-1830. doi: 10.1016/j.cclet.2020.11.031 shu

Molecular recognition triggered aptazyme cascade for ultrasensitive detection of exosomes in clinical serum samples

College of Chemistry, Green Catalysis Center, Henan Joint International Research Laboratory of Green Construction of Functional Molecules and Their Bioanalytical Applications, Zhengzhou Key Laboratory of Functional Nanomaterial and Medical Theranostic, Zhengzhou University, Zhengzhou 450001, China

hmmeng2017@zzu.edu.cn

zhaohui.li@zzu.edu.cn

Received Date: 5 September 2020
Revised Date: 20 October 2020
Accepted Date: 16 November 2020
Available Online: 20 November 2020

Figures(5)

Exosomes have attracted widespread interest due to their inherent advantages in tumor diagnosis and treatment monitoring. However, it is still a big challenge for highly sensitive and specific detection of exosome in real complexed samples. Herein, a molecular recognition triggered aptazyme cascade strategy was developed for ultrasensitive detection of cancer exosomes in clinical serum samples. In this design, one target exosome could capture a large quantity of aptazymes for the first-step signal amplification. And then the captured aptazyme was activated and recycled to release the fluorophore-abelled substrate strand for a cascaded signal amplification. Notably, the activation of aptazyme only occurs when it has bound with target exosome, ensuring a low background. The experimental results show that the limit of detection (LOD) and the limit of quantification (LOQ) are 3.5×10³ particles/μL and 1.7×10⁴ particles/μL, respectively, which is comparable to the results of most existed fluorescence-based exosome probes. Moreover, this assay possesses high specificity to distinguish exosomes derived from other cell lines. Furthermore, this fluorescence probe was utilized in cancer patient and healthy serum samples successfully, suggesting its great potential for clinical diagnosis and biological studies.
- Aptazyme,
- Exosomes,
- Fluorescence probe,
- Signal amplification,
- Clinical serum samples
1. [1]
  P. Zhang, M. He, Y. Zeng, Lab Chip 16(2016) 3033-3042. doi: 10.1039/C6LC00279J
2. [2]
  H. Xie, K. Di, R. Huang, et al., Chin. Chem. Lett. 31(2020) 1737-1745. doi: 10.1016/j.cclet.2020.02.049
3. [3]
  T. Pisitkun, R. F. Shen, M. A. Knepper, Proc. Natl. Acad. Sci. 101(2004) 13368-13373. doi: 10.1073/pnas.0403453101
4. [4]
  S. A. Melo, L. B. Luecke, C. Kahlert, et al., Nature 523(2015) 177-182. doi: 10.1038/nature14581
5. [5]
  J. Zou, M. Shi, X. Liu, et al., Anal. Chem. 91(2019) 2425-2430. doi: 10.1021/acs.analchem.8b05204
6. [6]
  B. Shao, Z. Xiao, Anal. Chim. Acta 1114(2020) 74-84. doi: 10.1016/j.aca.2020.02.041
7. [7]
  R. A. Dragovic, C. Gardiner, A. S. Brooks, et al., Nanomedicine Nanotech. Biol. Med. 7(2011) 780-788. doi: 10.1016/j.nano.2011.04.003
8. [8]
  E. van der Pol, F. A. W. Coumans, A. E. Grootemaat, et al., J. Thromb. Haemost. 12(2014) 1182-1192. doi: 10.1111/jth.12602
9. [9]
  H. Zhou, P. S. T. Yuen, T. Pisitkun, et al., Kidney Int. 69(2006) 1471-1476. doi: 10.1038/sj.ki.5000273
10. [10]
  M. Oliveira-Rodríguez, S. López-Cobo, H. T. Reyburn, et al., J. Extracell. Vesicles 5(2016) 31803-31812. doi: 10.3402/jev.v5.31803
11. [11]
  P. G. Moon, J. E. Lee, Y. E. Cho, et al., Clin. Cancer Res. 22(2016) 1757-1766. doi: 10.1158/1078-0432.CCR-15-0654
12. [12]
  V. Pospichalova, J. Svoboda, Z. Dave, et al., J. Extracell. Vesicles 4(2015) 25530-25544. doi: 10.3402/jev.v4.25530
13. [13]
  W. Shen, K. Guo, G. B. Adkins, et al., Angew. Chem. Int. Ed. 57(2018) 15675-15680. doi: 10.1002/anie.201806901
14. [14]
  J. Chen, H. M. Meng, Y. An, et al., Talanta 209(2020) 120510. doi: 10.1016/j.talanta.2019.120510
15. [15]
  J. Chen, J. Tang, H. M. Meng, et al., Chem. Commun. 56(2020) 9024-9027. doi: 10.1039/D0CC02337J
16. [16]
  Y. Xia, M. Liu, L. Wang, et al., Biosens. Bioelectron. 92(2017) 8-15. doi: 10.1016/j.bios.2017.01.063
17. [17]
  Z. Huang, Q. Lin, X. Ye, et al., Talanta 218(2020) 121089. doi: 10.1016/j.talanta.2020.121089
18. [18]
  W. Cheng, J. Ma, P. Cao, et al., Talanta 219(2020) 121242.
19. [19]
  Y. Cao, L. Li, B. Han, et al., Biosens. Bioelectron. 141(2019) 111397. doi: 10.1016/j.bios.2019.111397
20. [20]
  T. D. Li, R. Zhang, H. Chen, et al., Chem. Sci. 9(2018) 5372-5382. doi: 10.1039/C8SC01611A
21. [21]
  D. Ma, C. Huang, J. Zheng, et al., Biosens. Bioelectron. 101(2018) 167-173. doi: 10.1016/j.bios.2017.08.062
22. [22]
  E. I. Buzás, C. Gardiner, C. Lee, et al., Platelets 28(2017) 249-255. doi: 10.1080/09537104.2016.1260704
23. [23]
  X. X. Meng, X. H. Yang, K. M. Wang, et al., Chin. Chem. Lett. 20(2009) 990-994. doi: 10.1016/j.cclet.2009.03.028
24. [24]
  M. Wang, D. Yue, Q. Qiao, et al., Chin. Chem. Lett. 29(2018) 703-706. doi: 10.1016/j.cclet.2018.03.025
25. [25]
  Z. Zhang, A. Runa, J. Wu, et al., Chin. Chem. Lett. 30(2019) 779-782. doi: 10.1016/j.cclet.2018.10.019
26. [26]
  J. Huang, Y. He, X. H. Yang, et al., Chin. Chem. Lett. 25(2014) 1211-1214. doi: 10.1016/j.cclet.2014.05.039
27. [27]
  M. M. Ali, Y. Li, Angew. Chem. Int. Ed. 48(2009) 3512-3515. doi: 10.1002/anie.200805966
28. [28]
  P. Song, Y. Xiang, H. Xing, et al., Anal. Chem. 84(2012) 2916-2922. doi: 10.1021/ac203488p
29. [29]
  Y. Yang, J. Huang, X. Yang, et al., Anal. Chem. 88(2016) 5981-5987. doi: 10.1021/acs.analchem.6b00999
30. [30]
  D. Shangguan, Z. Cao, L. Meng, et al., J. Proteome Res. 7(2008) 2133-2139. doi: 10.1021/pr700894d
31. [31]
  W. Zhao, C. H. Cui, S. Bose, et al., Proc. Natl. Acad. Sci. 109(2012) 19626-19631. doi: 10.1073/pnas.1211234109
1. [1]
  Haijiang Gong , Qingtan Zeng , Shili Gai , Yaqian Du , Jing Zhang , Qingyu Wang , He Ding , Lichun Wu , Anees Ahmad Ansari , Piaoping Yang . Enzyme-based colorimetric signal amplification strategy in lateral flow immunoassay. Chinese Chemical Letters, 2025, 36(5): 110059-. doi: 10.1016/j.cclet.2024.110059
2. [2]
  Bo Liu , Shuaiqiang Shao , Junjie Cai , Zijian Zhang , Feng Tian , Kun Yang , Fan Li . Signal cascade amplification of streptavidin-biotin-modified immunofluorescence nanocapsules for ultrasensitive detection of glial fibrillary acidic protein. Chinese Chemical Letters, 2025, 36(3): 109814-. doi: 10.1016/j.cclet.2024.109814
3. [3]
  Shuo Tian , Shuyun Chen , Yunsen Wang , Dianping Tang . Liposomal photoelectrochemical immunoassay for low-abundance proteins with ternary transition metal sulfides for signal amplification. Chinese Chemical Letters, 2025, 36(7): 110418-. doi: 10.1016/j.cclet.2024.110418
4. [4]
  Quan Lu , Lulu Zhang , Zihan Chen , Jiajia Lv , Jie Gao , Xinmin Li , Hongyu Li , Wen Shi , Xiaohua Li , Huimin Ma , Zeli Yuan . A lipid droplet-targeting fluorescence probe for monitoring of lipid peroxidation in ferroptosis and non-alcoholic fatty liver disease. Chinese Chemical Letters, 2025, 36(8): 110620-. doi: 10.1016/j.cclet.2024.110620
5. [5]
  Jiayin Li , Linan Yang , Feng Shi , Yan Long , Yuru Wang , Daniel D. Stuart , Hongbo Li , Quan Cheng , Lingfeng Min , Zhanjun Yang , Juan Li . Versatile Au nanozyme Raman probe strategy for ultrasensitive encoded photonic crystal-based SERS multiplex immunosensing. Chinese Chemical Letters, 2025, 36(11): 110883-. doi: 10.1016/j.cclet.2025.110883
6. [6]
  Yang Xu , Le Ma , Yang Wang , Chunmeng Shi . Engineering strategies of biomaterial-assisted exosomes for skin wound repair: Latest advances and challenges. Chinese Chemical Letters, 2025, 36(1): 109766-. doi: 10.1016/j.cclet.2024.109766
7. [7]
  Haixian Ren , Yuting Du , Xiaojing Yang , Fangjun Huo , Le Zhang , Caixia Yin . Development of ESIPT-based specific fluorescent probes for bioactive species based on the protection-deprotection of the hydroxyl. Chinese Chemical Letters, 2025, 36(2): 109867-. doi: 10.1016/j.cclet.2024.109867
8. [8]
  Fengjie Liu , Fansu Meng , Zhenjiang Yang , Huan Wang , Yuehong Ren , Yu Cai , Xingwang Zhang . Exosome-biomimetic nanocarriers for oral drug delivery. Chinese Chemical Letters, 2024, 35(9): 109335-. doi: 10.1016/j.cclet.2023.109335
9. [9]
  Jingwen Zhao , Jianpu Tang , Zhen Cui , Limin Liu , Dayong Yang , Chi Yao . A DNA micro-complex containing polyaptamer for exosome separation and wound healing. Chinese Chemical Letters, 2024, 35(9): 109303-. doi: 10.1016/j.cclet.2023.109303
10. [10]
  Sixin Ai , Wenxiu Li , Huayong Zhu , Yang Wan , Weiying Lin . Viscosity-responsive signal amplification dual-modal probe triggered by cysteine/homocysteine for monitoring diabetic liver damages and repair processes. Chinese Chemical Letters, 2025, 36(3): 109904-. doi: 10.1016/j.cclet.2024.109904
11. [11]
  Qinghong Pan , Huafang Zhang , Qiaoling Liu , Donghong Huang , Da-Peng Yang , Tianjia Jiang , Shuyang Sun , Xiangrong Chen . A self-powered cathodic molecular imprinting ultrasensitive photoelectrochemical tetracycline sensor via ZnO/C photoanode signal amplification. Chinese Chemical Letters, 2025, 36(1): 110169-. doi: 10.1016/j.cclet.2024.110169
12. [12]
  Miao-Miao Chen , Min-Ling Zhang , Xiao Song , Jun Jiang , Xiaoqian Tang , Qi Zhang , Xiuhua Zhang , Peiwu Li . Smartphone-assisted electrochemiluminescence imaging test strips towards dual-signal visualized and sensitive monitoring of aflatoxin B1 in corn samples. Chinese Chemical Letters, 2025, 36(1): 109785-. doi: 10.1016/j.cclet.2024.109785
13. [13]
  Meiling Zhao , Yao Lu , Yutao Zhang , Haoyun Xue , Zhiqian Guo . Ultra-high signal-to-noise ratio near-infrared chemiluminescent probe for in vivo sensing singlet oxygen. Chinese Chemical Letters, 2025, 36(5): 110105-. doi: 10.1016/j.cclet.2024.110105
14. [14]
  Gaojian Yang , Zhiyang Li , Rabia Usman , Zhu Chen , Yuan Liu , Song Li , Hui Chen , Yan Deng , Yile Fang , Nongyue He . DNA walker induced "signal on" fluorescence aptasensor strategy for rapid and sensitive detection of extracellular vesicles in gastric cancer. Chinese Chemical Letters, 2025, 36(2): 109930-. doi: 10.1016/j.cclet.2024.109930
15. [15]
  Gongcheng Ma , Qihang Ding , Yuding Zhang , Yue Wang , Jingjing Xiang , Mingle Li , Qi Zhao , Saipeng Huang , Ping Gong , Jong Seung Kim . Palladium-free chemoselective probe for in vivo fluorescence imaging of carbon monoxide. Chinese Chemical Letters, 2024, 35(9): 109293-. doi: 10.1016/j.cclet.2023.109293
16. [16]
  Lingjun Sha , Bing Bo , Jiayu Li , Qi Liu , Ya Cao , Jing Zhao . Precise assessment of lung cancer-derived exosomes based on dual-labelled membrane interface. Chinese Chemical Letters, 2025, 36(4): 110109-. doi: 10.1016/j.cclet.2024.110109
17. [17]
  Yunyan Li , Zimin Cai , Zhicheng Wang , Sifeng Zhu , Wendian Liu , Cheng Wang . Construction of biomimetic hybrid nanovesicles based on M1 macrophage-derived exosomes for therapy of cancer. Chinese Chemical Letters, 2025, 36(4): 109942-. doi: 10.1016/j.cclet.2024.109942
18. [18]
  Yangyang Zhang , Lu Qiu , Houying Li , Wenli Cai , Ergang Liu , Hongtao Zhang , Bahtiyor Muhitdinov , Jianming Liang , Yongzhuo Huang . Biomimetically engineered plant-derived exosomes-like nanovesicles for rheumatoid arthritis therapy. Chinese Chemical Letters, 2025, 36(9): 110658-. doi: 10.1016/j.cclet.2024.110658
19. [19]
  Zhoupeng Zheng , Shengyi Gong , Qianhua Li , Shiya Zhang , Guoqiang Feng . Lipid droplets and gallbladder targeted fluorescence probe for ratiometric NO imaging in gallstones disease models. Chinese Chemical Letters, 2025, 36(5): 110191-. doi: 10.1016/j.cclet.2024.110191
20. [20]
  Luolin Wang , Xing Liang , Hanwen Chi , Weiying Lin . A dual-targeted near-infrared fluorescence lifetime probe for detecting viscosity heterogeneity in arthritic mice. Chinese Chemical Letters, 2025, 36(12): 110962-. doi: 10.1016/j.cclet.2025.110962

Get Citation

PDF

XML

Metrics

PDF Downloads(14)
Abstract views(1547)
HTML views(110)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142