Citation: Kangnan Wang, Wen Ma, Yuchun Xu, Xin Liu, Gui Chen, Meng Yu, Qiling Pan, Chaobo Huang, Xiaochuan Li, Qingchun Mu, Yongbing Sun, Zhiqiang Yu. Design of a novel mitochondria targetable turn-on fluorescence probe for hydrogen peroxide and its two-photon bioimaging applications[J]. Chinese Chemical Letters, ;2020, 31(12): 3149-3152. doi: 10.1016/j.cclet.2020.08.039 shu

Design of a novel mitochondria targetable turn-on fluorescence probe for hydrogen peroxide and its two-photon bioimaging applications

Kangnan Wang ^{a, 1} ,
Wen Ma ^{b, 1} ,
Yuchun Xu ^b ,
Xin Liu ^a ,
Gui Chen ^b ,
Meng Yu ^b ,
Qiling Pan ^a ,
Chaobo Huang ^c ,
Xiaochuan Li ^d ,
Qingchun Mu ^{d, *,} ,
Yongbing Sun ^{e, *,} ,
Zhiqiang Yu ^{b, *,}

a.
The First People's Hospital of Shunde, Southern Medical University, Foshan 528300, China
b.
School of Pharmaceutical Sciences, Southern Medical University, Guangzhou 510515, China
c.
Joint Laboratory of Advanced Biomedical Technology, College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, China
d.
The People's Hospital of Gaozhou, Gaozhou 525200, China
e.
Division of Pharmaceutics, National Pharmaceutical Engineering Center for Solid Preparation in Chinese Herbal Medicine, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China

muqcns@163.com

20101008@jxutcm.edu.cn

yuzq@smu.edu.cn

Received Date: 28 July 2020
Revised Date: 22 August 2020
Accepted Date: 24 August 2020
Available Online: 26 August 2020

Figures(4)

Considering that hydrogen peroxide (H₂O₂) plays significant roles in oxidative stress, the cellular signal transduction and essential biological process regulation, the detection and imaging of H₂O₂ in living systems undertakes critical responsibility. Herein, we have developed a novel two-photon fluorescence turn on probe, named as Pyp-B for mitochondria H₂O₂ detection in living systems. Selectivity studies show that probe Pyp-B exhibit highly sensitive response toward H₂O₂ than other reactive oxygen species (ROS) and reactive nitrogen species (RNS) as well as biologically relevant species. The fluorescence colocalization studies demonstrate that the probe can localize in the mitochondria solely. Furthermore, as a bio-compatibility molecule, the highly selective and sensitive of fluorescence probe Pyp-B have been confirmed by its cell imaging application of H₂O₂ in living A549 cells and zebrafishes under the physiological conditions.
- Hydrogen peroxide detection,
- p-Phenyl boronic acid ester,
- Mitochondria target,
- Two-photon fluorescence,
- Bioimaging
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