Citation: Lingyuan Liu, Shuyun Zhu, Jing Sun, Meng Xia, Xian'en Zhao, Guobao Xu. Ratiometric fluorescence detection of bleomycin based on proximity-dependent fluorescence conversion of DNA-templated silver nanoclusters[J]. Chinese Chemical Letters, ;2021, 32(2): 906-909. doi: 10.1016/j.cclet.2020.07.015 shu

Ratiometric fluorescence detection of bleomycin based on proximity-dependent fluorescence conversion of DNA-templated silver nanoclusters

Lingyuan Liu ^a ,
Shuyun Zhu ^{a, *,} ,
Jing Sun ^b ,
Meng Xia ^a ,
Xian'en Zhao ^{a, *,} ,
Guobao Xu ^c

a.
College of Chemistry and Chemical Engineering, Qufu Normal University, Qufu 273165, China
b.
Qinghai Key Laboratory of Qinghai-Tibet Plateau Biological Resources, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810001, China
c.
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China

shuyunzhu1981@163.com

xianenzhao@163.com

Received Date: 2 June 2020
Revised Date: 3 July 2020
Accepted Date: 8 July 2020
Available Online: 8 July 2020

Figures(3)

We design a ratiometric fluorescent sensing platform for bleomycin (BLM) by using proximity-dependent DNA-templated silver nanoclusters (DNA-AgNCs) probe. This ratiometric sensing system is constructed with DNA-AgNCs as single fluorophore. The proposed strategy is based on the two following facts: (1) a covert DNA can approach and transform the DNA-AgNCs with green emission (G-DNA-AgNCs) into red emission through hybridization reaction. (2) The specific cleavage of the convert DNA by BLM in the presence of Fe(II) inhibits the discoloration of G-DNA-AgNCs. Thus, benefiting from the specific recognition of BLM and unique properties of G-DNA-AgNCs, a highly-sensitive ratiometric sensor for BLM has been successfully developed. The detection limit is as low as 30 pmol/L. This label-free fluorescence probe possesses advantages of convenient synthetic process and low cost. Moreover, this ratiometric method has been applied to the detection of BLM in human serum samples, illustrating a promising tool for analysis of BLM in cancer therapy.
- Ratiometric nanosensor,
- DNA-templated silver nanoclusters,
- Fluorescence transformation,
- Bleomycin,
- DNA scission
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