Citation: Zhang Baoxin, Zhang Haijuan, Zhong Miao, Wang Song, Xu Qianhe, Cho Dong-Hyung, Qiu Hongdeng. A novel off-on fluorescent probe for specific detection and imaging of cysteine in live cells and in vivo[J]. Chinese Chemical Letters, ;2020, 31(1): 133-135. doi: 10.1016/j.cclet.2019.05.061 shu

A novel off-on fluorescent probe for specific detection and imaging of cysteine in live cells and in vivo

a.
State Key Laboratory of Applied Organic Chemistry and College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, China
b.
CAS Key Laboratory of Chemistry of Northwestern Plant Resources/Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, China
c.
School of Life Sciences, Kyungpook National University, Daegu 41566, Republic of Korea

zhanghj@licp.cas.cn

hdqiu@licp.cas.cn

Received Date: 11 April 2019
Revised Date: 27 May 2019
Accepted Date: 29 May 2019
Available Online: 30 January 2019

Figures(5)

Cysteine (Cys) plays a pivotal role in many physiological and pathological processes, including detoxification and protein synthesis. The abnormal levels of Cys are linked to many diseases. In this study, a novel red-emitting off-on fluorescent probe Cys-TCF was masterly constructed for discriminative detection of Cys. After a series of experimental assessment, Cys-TCF displayed higher selectivity and sensitivity for Cys over other biothilols with a low detection limit (0.04 μmol/L). More notably, the probe was also successfully applied to image Cys in live cells and live zebrafishes with low cytotoxicity.
- Cysteine,
- Fluorescent probe,
- Red emission,
- Low detection limit,
- Fluorescent imaging
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1.
沈阳化工大学材料科学与工程学院沈阳 110142