Citation: Huang Yuqin, Lv You, Geng Jia, Xiao Dan, Zhou Cuisong. A designed locked nucleic acid-based nanopore for discriminating ctDNA and its coexisting analogue ncDNA[J]. Chinese Chemical Letters, ;2020, 31(1): 172-176. doi: 10.1016/j.cclet.2019.05.033 shu

A designed locked nucleic acid-based nanopore for discriminating ctDNA and its coexisting analogue ncDNA

Huang Yuqin ^a ,
Lv You ^a ,
Geng Jia ^b ,
Xiao Dan ^a ,
Zhou Cuisong ^a,*,

a.
College of Chemistry, Sichuan University, Chengdu 610064, China
b.
Department of Laboratory Medicine, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University and Collaborative Innovation Center for Biotherapy, Chengdu 610041, China

zcs@scu.edu.cn

Received Date: 15 April 2019
Revised Date: 16 May 2019
Accepted Date: 17 May 2019
Available Online: 19 January 2019

Figures(4)

Circulating tumor DNA (ctDNA), carrying tumor-specific sequence mutations, is a promising biomarker for classification, diagnosis and prognosis of cancers. However, there is still a great challenge in discriminating single-base difference between ctDNA and its coexisting analogue (normal circulating DNA, ncDNA) at a serum sample. A locked nucleic acid (LNA) probe combined with α-HL nanopore sensor was designed, which achieved a high signal-to-background ratio (SBR) of ~8.34×10³, as well as a significant discrimination capability (~12.3 times) of single-base difference. The accurate discrimination strategy is label-free, convenient, selective and sensitive, which has great potential in the early diagnosis of diseases and biomedical research fields.
- Single-base difference,
- Simultaneous discrimination,
- Nanopore,
- Circulating tumor DNA,
- Locked nucleic acid
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