Citation: Wang Ruili, Lan Ling, Liu Li, Cheng Liang. Asymmetric polymerase chain reaction and loop-mediated isothermal amplification (AP-LAMP) for ultrasensitive detection of microRNAs[J]. Chinese Chemical Letters, ;2020, 31(1): 159-162. doi: 10.1016/j.cclet.2019.05.003 shu

Asymmetric polymerase chain reaction and loop-mediated isothermal amplification (AP-LAMP) for ultrasensitive detection of microRNAs

Wang Ruili ^a,b ,
Lan Ling ^a,b ,
Liu Li ^a,b ,
Cheng Liang ^a,b,*,

a.
Beijing National Laboratory for Molecular Sciences (BNLMS), CAS Key Laboratory of Molecular Recognition and Function, CAS Research/Education Centre for Excellence in Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China
b.
University of Chinese Academy of Sciences, Beijing 100049, China

chengl@iccas.ac.cn

Received Date: 15 April 2019
Revised Date: 30 April 2019
Accepted Date: 5 May 2019
Available Online: 7 January 2019

Figures(5)

In this manuscript, we first report an ultrasensitive detection assay of microRNA by combing asymmetric polymerase chain reaction (A-PCR) and loop-mediated isothermal amplification (LAMP) technology. Using A-PCR obtained an extended single strand to form LAMP stem-loop structure under isothermal amplification conditions. We used miRNAs as a loop primer probe in LAMP reaction and completed its ultrasensitive and rapid detection. The established method furnished a fast, specific and efficient detection of target miRNA with a detection limit as low as 10 amol/L in 90 min.
- microRNA,
- LAMP,
- Asymmetric PCR,
- Ultrasensitive detection,
- Rapid detection
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