Citation: Wang Ruili, Lan Ling, Liu Li, Cheng Liang. Asymmetric polymerase chain reaction and loop-mediated isothermal amplification (AP-LAMP) for ultrasensitive detection of microRNAs[J]. Chinese Chemical Letters, ;2020, 31(1): 159-162. doi: 10.1016/j.cclet.2019.05.003 shu

Asymmetric polymerase chain reaction and loop-mediated isothermal amplification (AP-LAMP) for ultrasensitive detection of microRNAs

Wang Ruili ^a,b ,
Lan Ling ^a,b ,
Liu Li ^a,b ,
Cheng Liang ^a,b,*,

a.
Beijing National Laboratory for Molecular Sciences (BNLMS), CAS Key Laboratory of Molecular Recognition and Function, CAS Research/Education Centre for Excellence in Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China
b.
University of Chinese Academy of Sciences, Beijing 100049, China

chengl@iccas.ac.cn

Received Date: 15 April 2019
Revised Date: 30 April 2019
Accepted Date: 5 May 2019
Available Online: 7 January 2019

Figures(5)

In this manuscript, we first report an ultrasensitive detection assay of microRNA by combing asymmetric polymerase chain reaction (A-PCR) and loop-mediated isothermal amplification (LAMP) technology. Using A-PCR obtained an extended single strand to form LAMP stem-loop structure under isothermal amplification conditions. We used miRNAs as a loop primer probe in LAMP reaction and completed its ultrasensitive and rapid detection. The established method furnished a fast, specific and efficient detection of target miRNA with a detection limit as low as 10 amol/L in 90 min.
- microRNA,
- LAMP,
- Asymmetric PCR,
- Ultrasensitive detection,
- Rapid detection
1. [1]
  R. Sunkar, Y.F. Li, G. Jagadeeswaran, Trends Plant Sci. 17(2012) 196-203. doi: 10.1016/j.tplants.2012.01.010
2. [2]
  K. Rogers, X. Chen, Plant Cell 25(2013) 2383-2399. doi: 10.1105/tpc.113.113159
3. [3]
  J.C. Carrington, V. Ambros, Science 301(2003) 336-338. doi: 10.1126/science.1085242
4. [4]
  Y. Moran, M. Agron, D. Praher, U. Technau, Nat. Ecol. Evol. 1(2017) 27. doi: 10.1038/s41559-016-0027
5. [5]
  R. Triboulet, B. Mari, Y.L. Lin, et al., Science 315(2007) 1579-1582. doi: 10.1126/science.1136319
6. [6]
  C.L. Jopling, M. Yi, A.M. Lancaster, S.M. Lemon, P. Sarnow, Science 309(2005) 1577-1581. doi: 10.1126/science.1113329
7. [7]
  S. Jonas, E. Izaurralde, Nat. Rev. Genet. 16(2015) 421-433. doi: 10.1038/nrg3965
8. [8]
  X. Wang, M. Li, Z. Wang, et al., J. Biol. Chem. 290(2015) 3925-3935. doi: 10.1074/jbc.M114.596866
9. [9]
  A. Molnár, F. Schwach, D.J. Studholme, E.C. Thuenemann, D.C. Baulcombe, Nature 447(2007) 1126-1129. doi: 10.1038/nature05903
10. [10]
  X. Yin, J. Hu, H. Xu, Chin. Chem. Lett. 29(2018) 1029-1032. doi: 10.1016/j.cclet.2018.04.027
11. [11]
  K.B. Reddy, Cancer Cell Int. 15(2015) 38. doi: 10.1186/s12935-015-0185-1
12. [12]
  Y. Liu, Q. Cai, P.P. Bao, et al., Breast Cancer Res. Treat. 152(2015) 183-191. doi: 10.1007/s10549-015-3460-x
13. [13]
  A. Bakkar, M. Alshalalfa, L.F. Petersen, et al., Mol. Biol. Rep. 43(2016) 229-240. doi: 10.1007/s11033-016-3948-4
14. [14]
  M.V. Iorio, P. Casalini, E. Tagliabue, S. Menard, C.M. Croce, Eur. J. Cancer 44(2008) 2753-2759. doi: 10.1016/j.ejca.2008.09.037
15. [15]
  D. Wu, X. Xie, A.A. Kadi, Y. Zhang, Chin. Chem. Lett. 29(2018) 1098-1104. doi: 10.1016/j.cclet.2018.04.030
16. [16]
  J. Fu, Z. Zhang, G. Li, Chin. Chem. Lett. 30(2019) 285-291. doi: 10.1016/j.cclet.2018.10.031
17. [17]
  T.T. Meng, Y.X. Liu, M.T. Liu, et al., Chin. Chem. Lett. 26(2015) 1179-1182. doi: 10.1016/j.cclet.2015.05.039
18. [18]
  X. Liu, M. Liu, J. Chen, Z. Li, Q. Yuan, Chin. Chem. Lett. 29(2018) 1321-1332. doi: 10.1016/j.cclet.2018.03.004
19. [19]
  C. Chen, D.A. Ridzon, A.J. Broomer, et al., Nucleic Acids Res. 33(2005) e179. doi: 10.1093/nar/gni178
20. [20]
  S. Sharbati-Tehrani, B. Kutz-Lohroff, R. Bergbauer, J. Scholven, R. Einspanier, BMC Mol. Biol. 9(2008) 34-34. doi: 10.1186/1471-2199-9-34
21. [21]
  S.P. Jonstrup, J. Koch, J. Kjems, RNA 12(2006) 1747-1752. doi: 10.1261/rna.110706
22. [22]
  Y. Cheng, X. Zhang, Z. Li, et al., Angew. Chem. Int. Ed. 48(2009) 3268-3272. doi: 10.1002/anie.200805665
23. [23]
  H. Liu, L. Li, L. Duan, et al., Anal. Chem. 85(2013) 7941-7947. doi: 10.1021/ac401715k
24. [24]
  R. Duan, X. Zuo, S. Wang, et al., J. Am. Chem. Soc. 135(2013) 4604-4607. doi: 10.1021/ja311313b
25. [25]
  X. Zhang, C. Liu, L. Sun, X. Duan, Z. Li, Chem. Sci. 6(2015) 6213-6218. doi: 10.1039/C5SC02641E
26. [26]
  H. Jia, Z. Li, C. Liu, Y. Cheng, Angew. Chem. Int. Ed. 49(2010) 5498-5501. doi: 10.1002/anie.201001375
27. [27]
  K. Wang, K. Zhang, Z. Lv, et al., Biosens. Bioelectron. 57(2014) 91-95. doi: 10.1016/j.bios.2014.01.058
28. [28]
  M. Zhou, X. Teng, Y. Li, R. Deng, J. Li, Anal. Chem. 91(2019) 5295-5302. doi: 10.1021/acs.analchem.9b00124
29. [29]
  C. Li, Z. Li, H. Jia, J. Yan, Chem. Commun. 47(2011) 2595-2597. doi: 10.1039/C0CC03957H
30. [30]
  W. Du, M. Lv, J. Li, R. Yu, J. Jiang, Chem. Commun. 52(2016) 12721-12724. doi: 10.1039/C6CC06160E
31. [31]
  Y. Sun, H. Tian, C. Liu, Y. Sun, Z. Li, Chem. Commun. 53(2017) 11040-11043. doi: 10.1039/C7CC06140D
32. [32]
  W. Tian, P. Li, W. He, C. Liu, Z. Li, Biosens. Bioelectron. 128(2019) 17-22. doi: 10.1016/j.bios.2018.12.041
33. [33]
  E. Koscianska, J. Starega-Roslan, L.J. Sznajder, et al., BMC Mol. Biol. 12(2011) 14. doi: 10.1186/1471-2199-12-14
34. [34]
  P.T. Nelson, D.A. Baldwin, L.M. Scearce, et al., Nat. Methods 1(2004) 155-161. doi: 10.1038/nmeth717
35. [35]
  T. Notomi, H. Okayama, H. Masubuchi, et al., Nucleic Acids Res. 28(2000) E63. doi: 10.1093/nar/28.12.e63
36. [36]
  N. Tomita, Y. Mori, H. Kanda, T. Notomi, Nat. Protoc. 3(2008) 877-882. doi: 10.1038/nprot.2008.57
37. [37]
  Y. Mori, T. Notomi, J. Infect. Chemother. 15(2009) 62-69. doi: 10.1007/s10156-009-0669-9
38. [38]
  K. Nagamine, T. Hase, T. Notomi, Mol. Cell Probes 16(2002) 223-229. doi: 10.1006/mcpr.2002.0415
39. [39]
  R. Wang, R. Zhao, Y. Li, et al., Lab Chip 18(2018) 3507-3515. doi: 10.1039/C8LC00841H
40. [40]
  K. Hsieh, P.L. Mage, A.T. Csordas, M. Eisenstein, H.T. Soh, Chem. Commun. 50(2014) 3747-3749. doi: 10.1039/c4cc00540f
41. [41]
  S. Xie, Y. Yuan, Y. Song, et al., Chem. Commun. 50(2014) 15932-15935. doi: 10.1039/C4CC06449F
42. [42]
  L.J. Xie, X.T. Yang, R.L. Wang, et al., Angew. Chem. Int. Ed. 58(2019) 5028-5032. doi: 10.1002/anie.201900901
43. [43]
  L.J. Xie, R.L. Wang, D. Wang, L. Liu, L. Cheng, Chem. Commun. 53(2017) 10734-10737. doi: 10.1039/C7CC05544G
44. [44]
  L. Cheng, K.G. Abhilash, R. Breslow, Proc. Natl. Acad. Sci. U. S. A. 109(2012) 12884-12887. doi: 10.1073/pnas.1210846109
45. [45]
  L. Cheng, A. Mahendran, R.L. Gonzalez Jr., R. Breslow, Proc. Natl. Acad. Sci. U. S. A. 111(2014) 7920-7924. doi: 10.1073/pnas.1407295111
46. [46]
  L. Cheng, C. Doubleday, R. Breslow, Proc. Natl. Acad. Sci. U. S. A. 112(2015) 4218-4220. doi: 10.1073/pnas.1503739112
47. [47]
  L. Li, L. Yuan, J. Luo, et al., Clin. Exp. Med. 13(2013) 109-117. doi: 10.1007/s10238-012-0186-5
48. [48]
  C. Eichelser, D. Flesch-Janys, J. Chang-Claude, K. Pantel, H. Schwarzenbach, Clin. Chem. 59(2013) 1489-1496. doi: 10.1373/clinchem.2013.205161
49. [49]
  G. Misso, M.T. Di Martino, G. de Rosa, et al., Mol. Ther. Nucleic Acids 3(2014) e194. doi: 10.1038/mtna.2014.46
50. [50]
  D. Lodygin, V. Tarasov, A. Epanchintsev, et al., Cell Cycle 7(2008) 2591-2600. doi: 10.4161/cc.7.16.6533
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