Citation: He Sitian, He Leiliang, Liu Beibei, Yu Songcheng, Liu Li'e, Tian Yongmei, Wang Jia, Ding Lihua, Wang Yilin, Qu Lingbo, Yu Fei, Wu Yongjun. Development of a rapid and sensitivity magnetic chemiluminescence immunoassay for DNA methyltransferase 1 in human serum[J]. Chinese Chemical Letters, ;2019, 30(5): 1031-1034. doi: 10.1016/j.cclet.2019.03.013 shu

Development of a rapid and sensitivity magnetic chemiluminescence immunoassay for DNA methyltransferase 1 in human serum

He Sitian ^a ,
He Leiliang ^a ,
Liu Beibei ^b ,
Yu Songcheng ^a ,
Liu Li'e ^a ,
Tian Yongmei ^a ,
Wang Jia ^a ,
Ding Lihua ^a ,
Wang Yilin ^a ,
Qu Lingbo ^c ,
Yu Fei ^a,*, ,
Wu Yongjun ^a,*,

a.
College of Public Health, Zhengzhou University, Zhengzhou 450001, China
b.
College of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou 450001, China
c.
Henan Joint International Research Laboratory of Green Construction of Functional Molecules and Their Bioanalytical Applications, Zhengzhou 450001, China

yufei@zzu.edu.cn

wuyongjun135@126.com

Received Date: 29 December 2018
Revised Date: 5 March 2019
Accepted Date: 6 March 2019
Available Online: 8 May 2019

Figures(5)

DNA methyltransferase 1 (DNMT1) is a useful biomarker for lung cancer in early clinical diagnosis. A rapid magnetic chemiluminescence immunoassay (MCLIA) for DNMT1 in human serum has been developed. Horseradish peroxidase(HRP)-second-Abwasused to labeled polyclonal antibodies of anti-DNMT1. DNMT1 in sample integrates with specific immunomagnetic beads and can constitute a supersandwiched immunoreaction. In magnetic field, nonspecific materials can be separated. After luminescent substrate luminol-H₂O₂-BIP was added, the relative light unit (RLU) of HRP was detected and was discovered to be directly proportional to the content of DNMT1 in sample. The correlative variables involved in the MCLIA valuewere optimized and the methodological evaluationwas carriedout. Afteroptimization, in the range of 0.5-128 ng/mL, the linear regression equation was y=0.5014x + 1.769 (x was logC_DNMT1, y was relative luminescence units (RLU)/RLU₀), and the limit of detection was 0.01 ng/mL. The RSD of intra- and interassays were 15.8%-16.9% and 14.3%-18.1%, respectively. The recovery was from 70.0% to 106.2%. Furthermore, paralleled with purchasable enzyme-linked immunosorbent assay (ELISA) kits, MCLEIA had lower detection limit, wider linear range and shorter detection time. Therefore, the MCLEIA established in this study could be used for the sensitive detection of DNMT1 in serum sample.
- DNA methyltransferase 1,
- Biomarker,
- Chemiluminescence immunoassay,
- Magnetic particles,
- Human serum
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沈阳化工大学材料科学与工程学院沈阳 110142