Citation: Hamada Naoki, Hashi Yuki, Yamaki Satoshi, Guo Yanli, Zhang Lin, Li Haifang, Lin Jin-Ming. Construction of on-line supercritical fluid extraction with reverse phase liquid chromatography-tandem mass spectrometry for the determination of capsaicin[J]. Chinese Chemical Letters, ;2019, 30(1): 99-102. doi: 10.1016/j.cclet.2018.10.029 shu

Construction of on-line supercritical fluid extraction with reverse phase liquid chromatography-tandem mass spectrometry for the determination of capsaicin

a.
Beijing Key Laboratory of Microanalytical Methods and Instrumentation, MOE Key Laboratory of Bioorganic Phosphorous Chemistry & Chemical Biology, Department of Chemistry, Tsinghua University, Beijing 100084, China
b.
China MS Center, Shimadzu(China) Co., Beijing Branch, Beijing 100020, China

Corresponding author: Lin Jin-Ming, jmlin@mail.tsinghua.edu.cn
Received Date: 31 July 2018
Revised Date: 24 October 2018
Accepted Date: 25 October 2018
Available Online: 26 January 2018

Figures(4)

A novel on-line system composed of supercritical fluid extraction (SFE), dilution line and reverse phase liquid chromatography/mass spectrometry (RPLC/MS) was constructed for on-line extraction and reverse phase separation of some fat-soluble components in foods. Three columns including a trap column, concentration column and analytical column were used for trapping the fat-soluble components, on-line enrichment and reverse phase separation, respectively. Capsaicinoids were on-line extracted by a CO₂ supercritical fluid, then concentrated and separated by using the C₁₈ columns, finally detected by mass spectrometry (MS). Capsaicin eluted at 10.1 min and limit of detection (LOD, S/N=3) for the standard solution is 0.55 pg. The linearity was calculated with a value of coefficient of determination (R²)≥0.998 in the range of 1.1-8.5 ng. Concentrations of capsaicin in the green, yellow, and red bell peppers were determined to be 60.33 ng/g, 31.79 ng/g, 35.38 ng/g, respectively.
- Supercritical fluid extraction,
- Liquid chromatography-mass spectrometry,
- Column-switching system,
- Capsaicin
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1.
沈阳化工大学材料科学与工程学院沈阳 110142