Citation: Zhou Maogui, Qin Shiya, Feng Zhenzhen, Song Chunxia, Zhang Hua, Li Wenshan, Wang Qing, Liu Jianbo, Huang Jin, Yang Xiaohai, Wang Kemin. A simple and sensitive assay for apurinic/apyrimidinic endonuclease 1 activity based on host-guest interaction of β-cyclodextrin polymer and pyrene[J]. Chinese Chemical Letters, ;2018, 29(6): 973-976. doi: 10.1016/j.cclet.2017.10.010 shu

A simple and sensitive assay for apurinic/apyrimidinic endonuclease 1 activity based on host-guest interaction of β-cyclodextrin polymer and pyrene

Zhou Maogui ^a,1 ,
Qin Shiya ^a,1 ,
Feng Zhenzhen ^a ,
Song Chunxia ^b ,
Zhang Hua ^a ,
Li Wenshan ^a ,
Wang Qing ^a ,
Liu Jianbo ^a ,
Huang Jin ^a ,
Yang Xiaohai ^a,, ,
Wang Kemin ^a,,

a.
State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province, Hunan University, Changsha 410082, China
b.
School of Science, Anhui Agricultural University, Hefei 230036, China

Corresponding author: Yang Xiaohai, yangxiaohai@hnu.edu.cn Wang Kemin, kmwang@hnu.edu.cn

Received Date: 31 May 2017
Revised Date: 9 October 2017
Accepted Date: 11 October 2017
Available Online: 13 June 2017

Figures(5)

Apurinic/apyrimidinic endonuclease 1 (APE1) plays an important role in protecting the fidelity of genetic material and is also a promising biomarker of cancers. Herein, a simple and sensitive fluorescent method for APE1 activity detection was designed based on host-guest interaction between β-cyclodextrin polymer (β-CDP) and pyrene. In this method, pyrene-labelled DNA probes (AP-S1S2) and β-CDP functioned as fluorescent signal producer and enhancer, respectively. When APE1 was absent, pyrene on the AP-S1S2 could not enter the cavity of β-CDP because of steric hindrance, leading to a weak fluorescence intensity/anisotropy. When APE1 was present, it would recognize and cleave AP site in APS1S2, thus producing a dissociated pyrene-labelled oligonucleotide segment. Pyrene labelled at the 5' end of oligonucleotide segment could be easily trapped into the cavity of β-CDP via host-guest interaction, leading to a significant enhancement of fluorescence intensity/anisotropy. This assay offered a dynamic range of 0.05 U/mL-5 U/mL and an estimated detection limit of 0.05 U/mL. Furthermore, assessment of APE1 activity in HeLa cell extracts was successfully implemented, which hold the potential for APE1-related cancer diagnosis and biological researches.
- APE1,
- Host-guest interaction,
- Fluorescence,
- β-Cyclodextrin polymer,
- Pyrene
1. [1]
  S. Roychoudhury, S. Nath, H. Song, et al., Mol. Cell. Biol. 37(2017) e00401-e00416.
2. [2]
  P.S. Guerreir, S.G. Estácio, F. Antunes, et al., Chem. Biol. Drug Des. 88(2016) 915-925. doi: 10.1111/cbdd.2016.88.issue-6
3. [3]
  Q. Kong, W. Wang, L. Luo, X. Sun, Obstet. Invest. 82(2017) 188-199. doi: 10.1159/000447261
4. [4]
  J.L. Illuzzi, D.R. Mcneill, P. Bastian, et al., Environ. Mol. Mutagen. 58(2017) 84-98. doi: 10.1002/em.v58.2
5. [5]
  S.S. Laev, N.F. Salakhutdinov, O.I. Lavrik, Bioorg. Med. Chem. 25(2017) 2531-2544. doi: 10.1016/j.bmc.2017.01.028
6. [6]
  T. Santana, M.C. Sá, E.M. Santos, et al., J. Oral Pathol. Med. 46(2017) 496-503. doi: 10.1111/jop.2017.46.issue-7
7. [7]
  M. Sahadevan, O. Lee, M. Muzzio, et al., Biomarkers 22(2017) 689-697.
8. [8]
  P.S. Guerreiro, E. Corvacho, J.G. Costa, et al., Chem. Biol. Drug Des. 90(2017) 561-571. doi: 10.1111/cbdd.2017.90.issue-4
9. [9]
  D.M. Wilson Ⅲ, J. Mol. Biol. 345(2005) 1003-1014. doi: 10.1016/j.jmb.2004.11.028
10. [10]
  Y. Zhuo, N. Liao, Y. Chai, et al., Anal. Chem. 86(2013) 1053-1060.
11. [11]
  L. Cesaratto, E. Codarin, C. Vascotto, et al., PLoS One 8(2013) e70909. doi: 10.1371/journal.pone.0070909
12. [12]
  J. Han, Y. Zhuo, Y. Chai, et al., Biosens. Bioelectron. 41(2013) 116-122. doi: 10.1016/j.bios.2012.07.082
13. [13]
  S. Fang, L. Chen, M. Zhao, Anal. Chem. 87(2015) 11952-11956. doi: 10.1021/acs.analchem.5b03939
14. [14]
  B. Liu, L. Peng, Anal. Methods 8(2016) 862-868. doi: 10.1039/C5AY02309B
15. [15]
  J. Zhai, Y. Liu, S. Huang, S. Fang, M. Zhao, Nucleic Acids Res. 37(2016) 3569-3579.
16. [16]
  M. Flaender, G. Costa, G. Nonglaton, C. Saint-Pierre, D. Gasrutto, Analyst 141(2016) 6208-6216. doi: 10.1039/C6AN01165A
17. [17]
  Y. Huang, Y. Ma, Y. Li, et al., New J. Chem. 41(2017) 1893-1896. doi: 10.1039/C6NJ03477B
18. [18]
  X. Ma, Y. Zhao, Chem. Rev. 115(2015) 7794-7839. doi: 10.1021/cr500392w
19. [19]
  C. Song, X. Yang, K. Wang, et al., Chem. Commun. 51(2015) 1815-1818. doi: 10.1039/C4CC08991J
20. [20]
  P. Liu, S. Sun, X. Guo, et al., Anal. Chem. 87(2015) 2665-2671. doi: 10.1021/ac503301q
21. [21]
  X. Guo, P. Liu, X. Yang, et al., Analyst 140(2015) 2016-2022. doi: 10.1039/C4AN02402H
22. [22]
  C. Koopmans, H. Ritter, Macromolecules 41(2008) 7418-7422. doi: 10.1021/ma801202f
23. [23]
  Y. Yu, Y. Liu, S.J. Zhen, C.Z. Huang, Chem. Commun. 49(2013) 1942-1944. doi: 10.1039/c3cc38129c
24. [24]
  L. Cui, Y. Zou, N. Lin, et al., Anal. Chem. 84(2012) 5535-5541. doi: 10.1021/ac300182w
1. [1]
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2. [2]
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13. [13]
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