Citation: Cai Honglian, Zhou Cuisong, Yang Qiufang, Ai Tingting, Huang Yuqin, Lv You, Geng Jia, Xiao Dan. Single-molecule investigation of human telomeric G-quadruplex interactions with Thioflavin T[J]. Chinese Chemical Letters, ;2018, 29(3): 531-534. doi: 10.1016/j.cclet.2017.09.010 shu

Single-molecule investigation of human telomeric G-quadruplex interactions with Thioflavin T

Cai Honglian ^a ,
Zhou Cuisong ^a,, ,
Yang Qiufang ^a ,
Ai Tingting ^a ,
Huang Yuqin ^a ,
Lv You ^a ,
Geng Jia ^b ,
Xiao Dan ^a,,

a.
College of Chemistry, Sichuan University, Chengdu 610064, China
b.
Department of Laboratory Medicine, West China Hospital, Sichuan University and Collaborative Innovation Center, Chengdu 610041, China

Corresponding author: Zhou Cuisong, zcs@scu.edu.cn Xiao Dan, xiaodan@scu.edu.cn
Received Date: 29 June 2017
Revised Date: 14 August 2017
Accepted Date: 4 September 2017
Available Online: 6 March 2017

Figures(4)

G-quadruplex ligands have been accepted as potential therapeutic agents for anticancer treatment. Thioflavin T (ThT), a highly selective G-quadruplex ligand, can bind G-quadruplex with a fluorescent light-up signal change and high specificity against DNA duplex. However, there are still different opinions that ThT induces/stabilizes G-quadruplex foldings/topologies in human telomere sequence. Here, a sensitive single-molecule nanopore technology was utilized to analyze the interactions between human telomeric DNA (Tel DNA) and ThT. Both translocation time and current blockade were measured to investigate the translocation behaviors. Furthermore, the effects of metal ion (K⁺ and Na⁺) and pH on the translocation behaviors were studied. Based on the single-molecule level analysis, there are some conclusions:(1) In the electrolyte solution containing 50 mmol/L KCl and 450 mmol/L NaCl, ThT can bind strongly with Tel DNA but nearly does not change the G-quadruplex form; (2) In the presence of high concentration K⁺, the ThT binding induces the structural change of hybrid G-quadruplex into antiparallel topology with an enhanced structural stability; (3) In either alkaline or acidic buffer, G-quadruplex form will change in certain degree. All above conclusions are helpful to deeply understand the interaction behaviors between Tel DNA and ThT. This nanopore platform, investigating G-quadruplex ligands at the single-molecule level, has great potential for the design of new drugs and sensors.
- α-Hemolysin nanopore,
- Interaction,
- Human telomere sequence,
- Thioflavin T,
- G-Quadruplex ligand
1. [1]
  S. Balasubramanian, S. Neidle, Curr. Opin. Chem. Biol. 13(2009) 345-353. doi: 10.1016/j.cbpa.2009.04.637
2. [2]
  N.W. Kim, M.A. Piatyszek, K.R. Prowse, et al., Science 266(1994) 2011-2015. doi: 10.1126/science.7605428
3. [3]
  A. Marchand, A. Granzhan, K. Iida, et al., J. Am. Chem. Soc. 137(2015) 750-756. doi: 10.1021/ja5099403
4. [4]
  J.M. Nicoludis, S.T. Miller, P.D. Jeffrey, et al., J. Am. Chem. Soc.134(2012) 20446-20456. doi: 10.1021/ja3088746
5. [5]
  J. Mohanty, N. Barooah, V. Dhamodharan, et al., J. Am. Chem. Soc. 135(2012) 367-376.
6. [6]
  A.R. de la Faverie, A. Guedin, A. Bedrat, L.A. Yatsunyk, J.L. Mergny, Nucleic Acids Res. 42(2014) 1-8. doi: 10.1093/nar/gkt1324
7. [7]
  D. Zhao, X.W. Dong, N. Jiang, D. Zhang, C.L. Liu, Nucleic Acids Res. 42(2014) 11612-11621. doi: 10.1093/nar/gku833
8. [8]
  V. Gabelica, R. Maeda, T. Fujimoto, et al., Biochemistry 52(2013) 5620-5628. doi: 10.1021/bi4006072
9. [9]
  L.L. Liu, Y. Shao, J. Peng, et al., Anal. Chem. 86(2014) 1622-1631. doi: 10.1021/ac403326m
10. [10]
  C. Cao, Y.L. Ying, Z.L. Hu, et al., Nat. Nanotechnol. 11(2016) 713-718. doi: 10.1038/nnano.2016.66
11. [11]
  Y.L. Ying, J.J. Zhang, R. Gao, Y.T. Long, Angew. Chem. Int. Ed. 52(2013) 13154-13161. doi: 10.1002/anie.201303529
12. [12]
  J. Geng, S. Wang, H. Fang, P.X. Guo, ACS Nano 7(2013) 3315-3323. doi: 10.1021/nn400020z
13. [13]
  J. Geng, K. Kim, J. Zhang, et al., Nature 514(2014) 612-615. doi: 10.1038/nature13817
14. [14]
  N. An, A.M. Fleming, C.J. Burrows, J. Am. Chem. Soc. 135(2013) 8562-8570. doi: 10.1021/ja400973m
15. [15]
  S. Hohng, S. Lee, J. Lee, M.H. Jo, Chem. Soc. Rev. 43(2014) 1007-1013. doi: 10.1039/C3CS60184F
16. [16]
  D. Koirala, S. Dhakal, B. Ashbridge, et al., Nat. Chem. 3(2011) 782-787. doi: 10.1038/nchem.1126
17. [17]
  J.W. Shim, L.Q. Gu, J. Phys. Chem. B 112(2008) 8354-8360. doi: 10.1021/jp0775911
18. [18]
  J.W. Shim, Q.L. Tan, L.Q. Gu, Nucleic Acids Res. 37(2009) 972-982. doi: 10.1093/nar/gkn968
19. [19]
  L.Z. Song, M.R. Hobaugh, C. Shustak, et al., Science 274(1996) 1859-1865. doi: 10.1126/science.274.5294.1859
20. [20]
  L. Zhang, K.X. Zhang, S. Rauf, et al., Anal. Chem. 88(2016) 4533-4540. doi: 10.1021/acs.analchem.6b00555
21. [21]
  Y.L. Ying, H.Y. Wang, T.C. Sutherl, Y.T. Long, Small 7(2011) 87-94. doi: 10.1002/smll.v7.1
22. [22]
  C. Yang, L. Liu, T. Zeng, et al., Anal. Chem. 85(2013) 7302-7307. doi: 10.1021/ac401198d
23. [23]
  Y.L. Ying, Y.T. Long, Sci. China Chem. 60(2017) 1187-1190. doi: 10.1007/s11426-017-9082-5
24. [24]
  R. Gao, Y.L. Ying, Y.X. Hu, Y.J. Li, Y.T. Long, Anal. Chem. 89(2017) 7382-7387. doi: 10.1021/acs.analchem.7b00729
25. [25]
  Y. Wang, D.J. Patel, Structure 1(1993) 263-282. doi: 10.1016/0969-2126(93)90015-9
26. [26]
  J.X. Dai, C. Punchihewa, A. Ambrus, et al., Nucleic Acids Res. 35(2007) 2440-2450. doi: 10.1093/nar/gkm009
27. [27]
  Y.L. Ying, D.W. Li, Y. Li, J.S. Lee, Y.T. Long, Chem. Commun. 47(2011) 5690-5692. doi: 10.1039/c0cc05787h
28. [28]
  K.W. Lim, S. Amrane, S. Bouaziz, et al., J. Am. Chem. Soc.131(2009) 4301-4309. doi: 10.1021/ja807503g
29. [29]
  A. Ambrus, D. Chen, J. Dai, et al., Nucleic Acids Res. 34(2006) 2723-2735. doi: 10.1093/nar/gkl348
30. [30]
  P. Murat, Y. Singh, E. Defrancq, Chem. Soc. Rev. 40(2011) 5293-5307. doi: 10.1039/c1cs15117g
31. [31]
  N. An, A.M. Fleming, E.G. Middleton, C.J. Burrows, Proc. Natl. Acad. Sci. U. S. A. 111(2014) 14325-14331. doi: 10.1073/pnas.1415944111
1. [1]
  Yuwen Zhu , Xiang Deng , Yan Wu , Baode Shen , Lingyu Hang , Yuye Xue , Hailong Yuan . Formation mechanism of herpetrione self-assembled nanoparticles based on pH-driven method. Chinese Chemical Letters, 2025, 36(1): 109733-. doi: 10.1016/j.cclet.2024.109733
2. [2]
  Zhibin Ren , Shan Li , Xiaoying Liu , Guanghao Lv , Lei Chen , Jingli Wang , Xingyi Li , Jiaqing Wang . Penetrating efficiency of supramolecular hydrogel eye drops: Electrostatic interaction surpasses ligand-receptor interaction. Chinese Chemical Letters, 2024, 35(11): 109629-. doi: 10.1016/j.cclet.2024.109629
3. [3]
  Saisai Yuan , Yiming Chen , Xijuan Wang , Degui Zhao , Tengyang Gao , Caiyun Wei , Chuanxiang Chen , Yang Yang , Wenjing Hong . Decouple the intermolecular interaction by encapsulating an insulating sheath. Chinese Chemical Letters, 2025, 36(6): 110816-. doi: 10.1016/j.cclet.2025.110816
4. [4]
  Cheng Wang , Ji Wang , Dong Liu , Zhi-Ling Zhang . Advances in virus-host interaction research based on microfluidic platforms. Chinese Chemical Letters, 2024, 35(12): 110302-. doi: 10.1016/j.cclet.2024.110302
5. [5]
  Jinli Chen , Shouquan Feng , Tianqi Yu , Yongjin Zou , Huan Wen , Shibin Yin . Modulating Metal-Support Interaction Between Pt3Ni and Unsaturated WOx to Selectively Regulate the ORR Performance. Chinese Journal of Structural Chemistry, 2023, 42(10): 100168-100168. doi: 10.1016/j.cjsc.2023.100168
6. [6]
  Wen Su , Siying Liu , Qingfu Zhang , Zhongyan Zhou , Na Wang , Lei Yue . Temperature-controlled electrospray ionization tandem mass spectrometry study on protein/small molecule interaction. Chinese Chemical Letters, 2025, 36(5): 110237-. doi: 10.1016/j.cclet.2024.110237
7. [7]
  Yanyu Jin , Wenzhe Si , Xing Yuan , Hongjun Cheng , Bin Zhou , Li Cai , Yu Wang , Qibao Wang , Junhua Li . Tuning TM–O interaction by acid etching in perovskite catalysts boosting catalytic performance. Chinese Chemical Letters, 2025, 36(5): 110260-. doi: 10.1016/j.cclet.2024.110260
8. [8]
  Ruofan Qi , Jing Zhang , Wang Sun , Bai Yu , Zhenhua Wang , Kening Sun . Solid-acid-Lewis-base interaction accelerates lithium ion transport for uniform lithium deposition. Chinese Chemical Letters, 2025, 36(6): 110009-. doi: 10.1016/j.cclet.2024.110009
9. [9]
  Yan-Bo Li , Yi Li , Liang Yin . Copper(Ⅰ)-catalyzed diastereodivergent construction of vicinal P-chiral and C-chiral centers facilitated by dual "soft-soft" interaction. Chinese Chemical Letters, 2024, 35(7): 109294-. doi: 10.1016/j.cclet.2023.109294
10. [10]
  Heng Gao , Zhaocong Cheng , Guangshui Tu , Zonglin Qiu , Xieyi Xiao , Haotian Zhou , Handou Zheng , Haiyang Gao . Thermally robust bis(imino)pyridyl iron catalysts for ethylene polymerization: Synergy effects of weak π-π interaction, steric bulk, and electronic tuning. Chinese Chemical Letters, 2025, 36(5): 110762-. doi: 10.1016/j.cclet.2024.110762
11. [11]
  Ran Zhu , Pan Zhang , Yitong Xu , Jiutong Ma , Qiong Jia . Design of host-guest interaction based molecularly imprinted polymers: Targeting recognition of the epitope of neuron-specific enolase via a SERS assay. Chinese Chemical Letters, 2025, 36(6): 110259-. doi: 10.1016/j.cclet.2024.110259
12. [12]
  Xinqiong Li , Guocheng Rao , Xi Peng , Chan Yang , Yanjing Zhang , Yan Tian , Xianghui Fu , Jia Geng . Direct detection of C9orf72 hexanucleotide repeat expansions by nanopore biosensor. Chinese Chemical Letters, 2024, 35(5): 109419-. doi: 10.1016/j.cclet.2023.109419
13. [13]
  Bohan Chen , Liming Gong , Jing Feng , Mingji Jin , Liqing Chen , Zhonggao Gao , Wei Huang . Research advances of nanoparticles for CAR-T therapy in solid tumors. Chinese Chemical Letters, 2024, 35(9): 109432-. doi: 10.1016/j.cclet.2023.109432
14. [14]
  Quan Zhang , Shunjie Xing , Jingqian Han , Li Feng , Jianchun Li , Zhaosheng Qian , Jin Zhou . Organic pollutant sensing for human health based on carbon dots. Chinese Chemical Letters, 2025, 36(1): 110117-. doi: 10.1016/j.cclet.2024.110117
15. [15]
  Yang Liu , Jing Liang , Mengzhu Zheng , Haoze Song , Lixia Chen , Hua Li . PD-L1/SHP2 dual PROTACs inhibit melanoma by enhancing T-cell killing activity. Chinese Chemical Letters, 2025, 36(6): 110317-. doi: 10.1016/j.cclet.2024.110317
16. [16]
  Kuangdi Luo , Yang Qin , Xuehao Zhang , Hanxu Ji , Heao Zhang , Jiangtian Li , Xianjin Xiao , Xinyu Wang . Regulable toehold lock for the effective control of strand displacement reaction sequence and circuit leakage. Chinese Chemical Letters, 2024, 35(7): 109104-. doi: 10.1016/j.cclet.2023.109104
17. [17]
  Min Huang , Ru Cheng , Shuai Wen , Liangtong Li , Jie Gao , Xiaohui Zhao , Chunmei Li , Hongyan Zou , Jian Wang . Ultrasensitive detection of microRNA-21 in human serum based on the confinement effect enhanced chemical etching of gold nanorods. Chinese Chemical Letters, 2024, 35(9): 109379-. doi: 10.1016/j.cclet.2023.109379
18. [18]
  Yi Li , Zhaoxiang Cao , Peng Liu , Xia Wu , Dongju Zhang . Revealing the Coloration and Color Change Mechanisms of the Eriochrome Black T Indicator through Computational Chemistry and UV-Visible Absorption Spectroscopy. University Chemistry, 2025, 40(3): 132-139. doi: 10.12461/PKU.DXHX202405154
19. [19]
  Pengyang FAN , Shan FAN , Qinjin DAI , Xiaoying ZHENG , Wei DONG , Mengxue WANG , Xiaoxiao HUANG , Yong ZHANG . Preparation and performance of rich 1T-MoS₂ nanosheets for high-performance aqueous zinc ion battery cathode materials. Chinese Journal of Inorganic Chemistry, 2025, 41(4): 675-682. doi: 10.11862/CJIC.20240339
20. [20]
  Zhenjie Yang , Chenyang Hu , Xuan Pang , Xuesi Chen . Sequence design in terpolymerization of ε-caprolactone, CO₂ and cyclohexane oxide: Random ester-carbonate distributions lead to large-span tunability. Chinese Chemical Letters, 2024, 35(5): 109340-. doi: 10.1016/j.cclet.2023.109340

Get Citation

PDF

XML

Metrics

PDF Downloads(1)
Abstract views(971)
HTML views(10)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142