Citation: Li Zhuang, He Jun, Zhang Yue, Wang Qing-Guo, Zhao Yan, Qu Hui-Hua, Wang Xue-Qian, Kong Hui, Zhang Wei-Ku, Xu Jie-Kun. Development of an enzyme-linked immunosorbent assay using specific monoclonal antibodies against theacrine and its application[J]. Chinese Chemical Letters, ;2016, 27(12): 1771-1775. doi: 10.1016/j.cclet.2016.06.052 shu

Development of an enzyme-linked immunosorbent assay using specific monoclonal antibodies against theacrine and its application

Li Zhuang ^a,b ,
He Jun ^c ,
Zhang Yue ^a,d ,
Wang Qing-Guo ^a,d ,
Zhao Yan ^a,d ,
Qu Hui-Hua ^a,d ,
Wang Xue-Qian ^a,d ,
Kong Hui ^a,d ,
Zhang Wei-Ku ^b,, ,
Xu Jie-Kun ^a,d,,

a.
School of Basic Medical Sciences, Beijing University of Chinese Medicine, Beijing 100029, China
b.
Department of Pharmacology, Institute of Clinical Medical Sciences, China-Japan Friendship Hospital, Beijing 100029, China
c.
Department of Pharmacy, China-Japan Friendship Hospital, Beijing 100029, China
d.
Classical Prescription Application Foundation Research Innovation Team, Beijing University of Chinese Medicine, Beijing 100029, China

Corresponding author: Zhang Wei-Ku, cpuzwk@163.com Xu Jie-Kun, xjkbucm@163.com
Received Date: 16 May 2016
Revised Date: 13 June 2016
Accepted Date: 21 June 2016
Available Online: 16 December 2016

Figures(4)

Theacrine (l, 3, 7, 9-tetramethyluric acid), a purine alkaloid similar to caffeine in its chemical structure, is isolated from edible Camellia assamica var. kucha and has various pharmacological activities including hypnotic effects, anti-depressant effects, anti-inflammatory and analgesic effects, and a protective effect against stress-provoked liver damage. A rapid and simple assay is required to quantify theacrine in biological samples for pharmacokinetic studies in small animals. This study aimed to establish an enzyme-linked immunosorbent assay (ELISA) for theacrine quantification in blood. Herein, we successfully obtained monoclonal antibodies (MAbs) against theacrine, MAbs C11B5, and developed an ELISA method for the fast determination of theacrine in mouse blood. The range for calibration of theacrine by ELISA was 0.156-100 μg mL^-1. The half maximuminhibitory concentration (IC₅₀) value was 1.55 μg mL^-1. The ELISA method lays a good foundation for the further research.
- Theacrine,
- Theophylline-7-acetic acid,
- ELISA,
- Monoclonal antibody
1. [1]
  H.D. Zhang. A revision on the tea resource plants[J]. Acta Scientiarum Naturalium Universitatis Sunyatseni, 1984,23:3-14.
2. [2]
  C.X. Ye, Y.C. Lin, J.Y. Su, X.H. Song, H.D. Zhang. Purine alkaloids in Camellia assamica var. kucha Chang et Wang[J]. Acta Scientiarum Naturalium Universitatis Sunyatseni, 1999,38:82-86.
3. [3]
  T.B. Johnson. Purines in the plant kingdom:the discovery of a new purine in tea[J]. J. Am. Chem. Soc., 1937,59:1261-1264. doi: 10.1021/ja01286a030
4. [4]
  J.B. Petermann, T.W. Baumann. Metabolic relations between methylxanthines and methyluric acids in Coffea L[J]. Plant Physiol., 1983,73:961-964. doi: 10.1104/pp.73.4.961
5. [5]
  A. Smith, C. Brice, J. Nash, N. Rich, D.J. Nutt. Caffeine and central noradrenaline:effects on mood, cognitive performance, eye movements and cardiovascular function[J]. J. Psychopharmacol., 2003,17:283-292. doi: 10.1177/02698811030173010
6. [6]
  D. Shi, J.W. Daly. Chronic effects of xanthines on levels of central receptors in mice[J]. Cell. Mol. Neurobiol., 1999,19:719-732. doi: 10.1023/A:1006901005925
7. [7]
  J.K. Xu, H. Kurihara, L. Zhao, X.S. Yao. Theacrine, a special purine alkaloid with sedative and hypnotic properties from Cammelia assamica var. kucha in mice[J]. J. Asian Nat. Prod. Res., 2007,9:665-672. doi: 10.1080/10286020601103155
8. [8]
  G. Xie, M.Z. Wu, Y.R. Huang. Experimental study of theacrine on antidepressant effects[J]. Chin. Pharmacol. Bull., 2009,25:1160-1163.
9. [9]
  Y.Y. Wang, X.R. Yang, X.Q. Zheng. Theacrine, a purine alkaloid with antiinflammatory and analgesic activities[J]. Fitoterapia, 2010,81:627-631. doi: 10.1016/j.fitote.2010.03.008
10. [10]
  W.X. Li, Y.F. Li, Y.J. Zhai. Theacrine, a purine alkaloid obtained from Camellia assamica var. kucha, attenuates restraint stress-provoked liver damage in mice[J]. J. Agric. Food Chem., 2013,61:6328-6335. doi: 10.1021/jf400982c
11. [11]
  A.A. Feduccia, Y.Y. Wang, J.A. Simms. Locomotor activation by theacrine, a purine alkaloid structurally similar to caffeine:involvement of adenosine and dopamine receptors[J]. Pharmacol. Biochem. Behav., 2012,102:241-248. doi: 10.1016/j.pbb.2012.04.014
12. [12]
  W.K. Zhang, J.K. Xu, J.Q. Hu. Determination of theacrine in rat plasma by RPHPLC[J]. China J. Chin. Mat. Med., 2013,38:753-756.
13. [13]
  D.M. Wang, L.L. Du, J.L. Lu. HPLC-DAD/MS analysis of tea polyphenols in Camellia assamica var. kucha[J]. Nat. Prod. Res. Dev., 2006,18:978-981.
14. [14]
  N. Fukuda, H. Tanaka, Y. Shoyama. Formation of monoclonal antibody against a major ginseng component, ginsenoside Rg1 and its characterization. Monoclonal antibody for a ginseng saponin[J]. Cytotechnology, 2000,34:197-204. doi: 10.1023/A:1008162703957
1. [1]
  Mengxing Liu , Jing Liu , Hongxing Zhang , Jianan Tao , Peiwen Fan , Xin Lv , Wei Guo . One-pot accessing of meso–aryl heptamethine indocyanine NIR fluorophores and potential application in developing dye-antibody conjugate for imaging tumor. Chinese Chemical Letters, 2025, 36(4): 109994-. doi: 10.1016/j.cclet.2024.109994
2. [2]
  . . University Chemistry, 2024, 39(7): 0-0.
3. [3]
  Yan Guo , Hongtao Bian , Le Yu , Jiani Ma , Yu Fang . Photochemical reaction mechanism of benzophenone protected guanosine at N7 position. Chinese Chemical Letters, 2025, 36(3): 109971-. doi: 10.1016/j.cclet.2024.109971
4. [4]
  . . University Chemistry, 2025, 40(7): 0-0.
5. [5]
  . 第41卷第7期封面和目次. Acta Physico-Chimica Sinica, 2025, 41(7): -.
6. [6]
  Wenxiong Yu , Chenyu Yang , Xian Feng , Chengshuo Shen . Scholl cyclization of [6]helicenes into negatively curved hexa[7]circulenes. Chinese Chemical Letters, 2025, 36(11): 110939-. doi: 10.1016/j.cclet.2025.110939
7. [7]
  Qingxuan Kong , Changwei Jiang , Bin Lyu , Zhaoting Li , Ning Jiao , Song Song . Denitrative iodination of nitroarenes with hydroiodic acid. Chinese Chemical Letters, 2025, 36(10): 111444-. doi: 10.1016/j.cclet.2025.111444
8. [8]
  Yiran Tao , Chunlei Dai , Zhaoxiang Xie , Xinru You , Kaiwen Li , Jun Wu , Hai Huang . Redox responsive polymeric nanoparticles enhance the efficacy of cyclin dependent kinase 7 inhibitor for enhanced treatment of prostate cancer. Chinese Chemical Letters, 2024, 35(8): 109170-. doi: 10.1016/j.cclet.2023.109170
9. [9]
  Hui-Juan Wang , Wen-Wen Xing , Zhen-Hai Yu , Yong-Xue Li , Heng-Yi Zhang , Qilin Yu , Hongjie Zhu , Yao-Yao Wang , Yu Liu . Cucurbit[7]uril confined phenothiazine bridged bis(bromophenyl pyridine) activated NIR luminescence for lysosome imaging. Chinese Chemical Letters, 2024, 35(6): 109183-. doi: 10.1016/j.cclet.2023.109183
10. [10]
  Qin Wang , Han Luo , Luli Wang , Ling Huang , Liling Cao , Xuehua Dong , Guohong Zou . KSb₂F₇·2KNO₃: Unveiling the peak birefringence in inorganic antimony oxysalts. Chinese Chemical Letters, 2025, 36(7): 110173-. doi: 10.1016/j.cclet.2024.110173
11. [11]
  Jing Guo , Zhi-Guo Lu , Rui-Chen Zhao , Bao-Ku Li , Xin Zhang . Nucleic acid therapy for metabolic-related diseases. Chinese Chemical Letters, 2025, 36(3): 109875-. doi: 10.1016/j.cclet.2024.109875
12. [12]
  Xiying Wu , Anze Liu , Yuzhong Yan , Ying Lu , Huan Wang . Folic acid ameliorates the immunogenicity of PEGylated liposomes. Chinese Chemical Letters, 2025, 36(6): 110285-. doi: 10.1016/j.cclet.2024.110285
13. [13]
  Jialin Guo , Mingrui Gu , Yahui Chen , Tao Xiong , Yiyang Zhang , Simin Chen , Mingle Li , Xiaoqiang Chen , Xiaojun Peng . Nucleic acid delivery by lipid nanoparticles for organ targeting. Chinese Chemical Letters, 2025, 36(11): 110849-. doi: 10.1016/j.cclet.2025.110849
14. [14]
  Shulei Hu , Yu Zhang , Xiong Xie , Luhan Li , Kaixian Chen , Hong Liu , Jiang Wang . Rh(Ⅲ)-catalyzed late-stage C-H alkenylation and macrolactamization for the synthesis of cyclic peptides with unique Trp(C7)-alkene crosslinks. Chinese Chemical Letters, 2024, 35(8): 109408-. doi: 10.1016/j.cclet.2023.109408
15. [15]
  Zhilian Liu , Wengui Wang , Hongxiao Yang , Yu Cui , Shoufeng Wang . Ideological and Political Education Design for the Synthesis of Irinotecan Drug Intermediate 7-Ethyl Camptothecin. University Chemistry, 2024, 39(2): 89-93. doi: 10.3866/PKU.DXHX202306012
16. [16]
  Hao Zhang , Haonan Qu , Ehsan Bahojb Noruzi , Haibing Li , Feng Liang . A nanocomposite film with layer-by-layer self-assembled gold nanospheres driven by cucurbit[7]uril for the selective transport of L-tryptophan and lysozyme. Chinese Chemical Letters, 2025, 36(1): 109731-. doi: 10.1016/j.cclet.2024.109731
17. [17]
  Hongzhi Zhang , Hong Li , Asif Ali Haider , Junpeng Li , Zhi Xie , Hongming Jiang , Conglin Liu , Rui Wang , Jing Zhu . An unexpected role of lanthanide substitution in thermally responsive phosphors NaLnTe2O7: Eu3+ (Ln = Y and Gd). Chinese Journal of Structural Chemistry, 2025, 44(2): 100509-100509. doi: 10.1016/j.cjsc.2024.100509
18. [18]
  Pei-Pei Liu , Jia-Bin Xing , Yue-Yang Liu , Ke Feng , Hui Wang , Dan-Wei Zhang , Wei Zhou , Gang Zhao , Jiaheng Zhang , Zhan-Ting Li . Sulfonatoproxylated cucurbit[7]urils as highly water-soluble and biocompatible excipients for solubilizing poorly soluble drugs and improving the bioavailability of indomethacin. Chinese Chemical Letters, 2025, 36(9): 110831-. doi: 10.1016/j.cclet.2025.110831
19. [19]
  Miao-Bin Xu , Qian-Qian Chen , Bing-Xuan Li , Ke-Zhao Du , Jin Chen . π-Lone pair synergy in (C₇H₄NO₄)(IO₃): Optimal balance among SHG, birefringence, and bandgap performance. Chinese Chemical Letters, 2025, 36(11): 110513-. doi: 10.1016/j.cclet.2024.110513
20. [20]
  Wenyi Mei , Lijuan Xie , Xiaodong Zhang , Cunjian Shi , Fengzhi Wang , Qiqi Fu , Zhenjiang Zhao , Honglin Li , Yufang Xu , Zhuo Chen . Design, synthesis and biological evaluation of fluorescent derivatives of ursolic acid in living cells. Chinese Chemical Letters, 2024, 35(5): 108825-. doi: 10.1016/j.cclet.2023.108825

Get Citation

PDF

XML

Metrics

PDF Downloads(0)
Abstract views(1345)
HTML views(34)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142