Citation: Feng Wei, Qiao Qing-Long, Leng Shuang, Miao Lu, Yin Wen-Ting, Wang Li-Qiu, Xu Zhao-Chao. A 1, 8-naphthalimide-derived turn-on fluorescent probe for imaging lysosomal nitric oxide in living cells[J]. Chinese Chemical Letters, ;2016, 27(9): 1554-1558. doi: 10.1016/j.cclet.2016.06.016 shu

A 1, 8-naphthalimide-derived turn-on fluorescent probe for imaging lysosomal nitric oxide in living cells

Feng Wei ^a,b ,
Qiao Qing-Long ^b,c ,
Leng Shuang ^b ,
Miao Lu ^b ,
Yin Wen-Ting ^b ,
Wang Li-Qiu ^a,, ,
Xu Zhao-Chao ^b,,

a.
College of Environmental and Chemical Engineering, Yanshan University, Qinhuangdao 066004, China
b.
State Key Laboratory of Fine Chemicals, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China
c.
State Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian 116012, China

Corresponding author: Wang Li-Qiu, liqiuwang@tom.com Xu Zhao-Chao, zcxu@dicp.ac.cn
Received Date: 22 April 2016
Revised Date: 10 May 2016
Accepted Date: 16 May 2016
Available Online: 17 September 2016

Figures(7)

Nitric oxide has played an important role in many physiological and pathological processes as a kind of important gas signal molecules. In this work, a new fluorescent probe LysoNO-Naph for detecting NO in lysosomes based on 1, 8-naphthalimide was reported. LysoNO-Naph has sub-groups of o-phenylenediamine as a NO reaction site and 4-(2-aminoethyl)-morpholine as a lysosome-targetable group. This probe exhibited good selectivity and high sensitivity (4.57 μmol/L) toward NO in a wide pH range from 4 to 12. Furthermore, LysoNO-Naph can be used for imaging NO in lysosomes in living cells.
- Fluorescent probe,
- Nitric oxide,
- Lysosome localized,
- Imaging in living cells
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