Citation: Maturavongsadit Panita, Bi Xiangdong, Gado TogorA., Nie Yu-Zhe, Qian Wang. Adhesive peptides conjugated PAMAM dendrimer as a coating polymeric material enhancing cell responses[J]. Chinese Chemical Letters, ;2016, 27(9): 1473-1478. doi: 10.1016/j.cclet.2016.03.012 shu

Adhesive peptides conjugated PAMAM dendrimer as a coating polymeric material enhancing cell responses

a.
Department of Chemistry and Biochemistry, University of South Carolina, Columbia, SC 29208, United States
b.
Department of Physical Sciences, Charleston Southern University, Charleston, SC 29423, United States

Corresponding author: Qian Wang, wang263@mailbox.sc.edu
Received Date: 13 February 2016
Revised Date: 29 February 2016
Accepted Date: 4 March 2016
Available Online: 5 September 2016

Figures(4)

This present work aims to functionalize poly(amidoamine) (PAMAM) dendrimers with various reported adhesive peptides, including Arg-Gly-Asp (RGD), Tyr-Ile-Gly-Ser-Arg (YIGSR), and Ile-Lys-Val-Ala-Val (IKVAV) for enhancing cell responses. The RGD, YIGSR, or IKVAV functionalized PAMAM coated substrate could promote cell adhesion of bone marrow mesenchymal stem cells (BMSCs) within 1 h after incubation. The neurite differentiation and proliferation of pheochromocytoma (PC12) cells were also significantly enhanced after culturing on the peptide functionalized PAMAM dendrimers for two and four days. This peptide functionalized PAMAM dendrimers are considered as the potential candidates for various tissue engineering applications.
- PAMAM dendrimer,
- RGD peptide,
- YIGSR peptide,
- IKVAV peptide,
- Bone marrow mesenchymal stem cells,
- PC12 cells
1. [1]
  Tomalia D.A., Naylor A.M., Goddard W.A.. Starburst dendrimers:molecular-level control of size, shape, surface chemistry, topology, and flexibility from atoms to macroscopic matter[J]. Angew. Chem. Int. Ed., 1990,29:138-175. doi: 10.1002/(ISSN)1521-3773
2. [2]
  Huang B.H., Otis J., Joice M., Kotlyar A., Thomas T.P.. PSMA-targeted stably linked "Dendrimer-Glutamate Urea-Methotrexate" as a prostate cancer therapeutic[J]. Biomacromolecules, 2014,15:915-923. doi: 10.1021/bm401777w
3. [3]
  Peng J.Q., Qi X.L., Chen Y.. Octreotide-conjugated PAMAM for targeted delivery to somatostatin receptors over-expressed tumor cells[J]. J. Drug Target, 2014,22:428-438. doi: 10.3109/1061186X.2013.879386
4. [4]
  Dib N., Fernández L., Gonzalez M.. Evaluation of different PAMAM dendrimers as molecular vehicle of 1, 2, 4-triazine N-oxide derivative with potential antitumor activity[J]. J. Inclusion Phenom. Macrocyclic Chem., 2014,79:65-73. doi: 10.1007/s10847-013-0324-z
5. [5]
  Prieto M.J., Del Rio Zabala N.E., Marotta C.H.. Optimization and in vivo toxicity evaluation of G4.5 pamam dendrimer-risperidone complexes[J]. PLoS ONE, 2014,9e90393. doi: 10.1371/journal.pone.0090393
6. [6]
  Kim T.H., Seo H.W., Han J., Ko K.S., Choi J.S.. Polyethylenimine-grafted polyamidoamine conjugates for gene delivery with high efficiency and low cytotoxicity[J]. Macromol. Res., 2014,22:757-764. doi: 10.1007/s13233-014-2108-8
7. [7]
  Liu X.X., Liu C., Catapano C.V.. Structurally flexible triethanolamine-core poly(amidoamine) dendrimers as effective nanovectors to deliver RNAi-based therapeutics[J]. Biotechnol. Adv., 2014,32:844-852. doi: 10.1016/j.biotechadv.2013.08.001
8. [8]
  Liu H., Zhu J.Y., Zhao J.L., Zhang G.X., Shi X.Y.. Targeted dendrimer-stabilized gold nanoparticles for computed tomography imaging of cancer cells[J]. J. Controlled Release, 2013,172:e37-e38.
9. [9]
  Liu J.F., Liu J.J., Chu L.P.. Synthesis, biodistribution, and imaging of PEGylatedacetylated polyamidoamine dendrimers[J]. J. Nanosci. Nanotechnol., 2014,14:3305-3312. doi: 10.1166/jnn.2014.7995
10. [10]
  Jiang L.Y., Lv B., Luo Y.. The effects of an RGD-PAMAM dendrimer conjugate in 3D spheroid culture on cell proliferation, expression and aggregation[J]. Biomaterials, 2013,34:2665-2673. doi: 10.1016/j.biomaterials.2013.01.003
11. [11]
  Iwamoto Y., Robey F.A., Graf J.. YIGSR, a synthetic laminin pentapeptide, inhibits experimental metastasis formation[J]. Science, 1987,238:1132-1134. doi: 10.1126/science.2961059
12. [12]
  Wu Y.C., Zhang Q.X., Du J.Y.. Self-assembled IKVAV peptide nanofibers promote adherence of PC12 cells[J]. J. Huazhong Univ. Sci. Technol., 2006,26:594-596. doi: 10.1007/s11596-006-0530-7
13. [13]
  Sekiya I., Vuoristo J.T., Larson B.L., Prockop D.J.. In vitro cartilage formation by human adult stem cells from bone marrow stroma defines the sequence of cellular and molecular events during chondrogenesis[J]. Proc. Natl. Acad. Sci. U.S.A., 2002,99:4397-4402. doi: 10.1073/pnas.052716199
14. [14]
  Mackay A.M., Beck S.C., Murphy J.M.. Chondrogenic differentiation of cultured human mesenchymal stem cells from marrow[J]. Tissue Eng., 1998,4:415-428. doi: 10.1089/ten.1998.4.415
15. [15]
  Boeuf S., Richter W.. Chondrogenesis of mesenchymal stem cells:role of tissue source and inducing factors[J]. Stem Cell Res. Ther., 2010,131. doi: 10.1186/scrt31
16. [16]
  Pollock J.D., Krempin M., Rudy B.. Differential effects of NGF, FGF, EGF, cAMP, and dexamethasone on neurite outgrowth and sodium channel expression in PC12 cells[J]. J. Neurosci., 1990,10:2626-2637.
17. [17]
  Tomaselli K.J., Damsky C.H., Reichardt L.F.. Interactions of a neuronal cell line (PC12) with laminin, collagen IV, and fibronectin:identification of integrinrelated glycoproteins involved in attachment and process outgrowth[J]. J. Cell Biol., 1987,105:2347-2358. doi: 10.1083/jcb.105.5.2347
18. [18]
  Bellis S.L.. Advantages of RGD peptides for directing cell association with biomaterials[J]. Biomaterials, 2011,32:4205-4210. doi: 10.1016/j.biomaterials.2011.02.029
19. [19]
  Hersel U., Dahmen C., Kessler H.. RGD modified polymers:biomaterials for stimulated cell adhesion and beyond[J]. Biomaterials, 2003,24:4385-4415. doi: 10.1016/S0142-9612(03)00343-0
20. [20]
  Tashiro K., Sephel G.C., Weeks B.. A synthetic peptide containing the IKVAV sequence from the A chain of laminin mediates cell attachment, migration, and neurite outgrowth[J]. J. Biol. Chem., 1989,264:16174-16182.
21. [21]
  Shin H., Jo S., Mikos A.G.. Biomimetic materials for tissue engineering[J]. Biomaterials, 2003,24:4353-4364. doi: 10.1016/S0142-9612(03)00339-9
22. [22]
  Lee L.A., Nguyen Q.L., Wu L.Y.. Mutant plant viruses with cell binding motifs provide differential adhesion strengths and morphologies[J]. Biomacromolecules, 2012,13:422-431. doi: 10.1021/bm2014558
23. [23]
  Kaur G., Wang C., Sun J., Wang Q.. The synergistic effects of multivalent ligand display and nanotopography on osteogenic differentiation of rat bone marrow stem cells[J]. Biomaterials, 2010,31:5813-5824. doi: 10.1016/j.biomaterials.2010.04.017
24. [24]
  Luckanagul J., Lee L.A., Nguyen Q.L.. Porous alginate hydrogel functionalized with virus as three-dimensional scaffolds for bone differentiation[J]. Biomacromolecules, 2012,13:3949-3958. doi: 10.1021/bm301180c
25. [25]
  Chung T.W., Yang M.G., Liu D.Z.. Enhancing growth human endothelial cells on Arg-Gly-Asp (RGD) embedded poly(e-caprolactone) (PCL) surface with nanometer scale of surface disturbance[J]. J. Biomed. Mater. Res., 2005,A 72A:213-219.
26. [26]
  Chung T.W., Lai D.M., Chen S.D., Lin Y.I.. Poly(e-caprolactone) scaffolds functionalized by grafting NGF and GRGD promote growth and differentiation of PC12 cells[J]. J. Biomed. Mater. Res., A, 2014,102:315-323. doi: 10.1002/jbm.a.v102.2
27. [27]
  Lutolf M.P., Hubbell J.A.. Synthetic biomaterials as instructive extracellular microenvironments for morphogenesis in tissue engineering[J]. Nat. Biotechnol., 2005,23:47-55. doi: 10.1038/nbt1055
28. [28]
  Zhu D.W., Chen Z., Zhao K.Y.. Polypropylene non-woven supported fibronectin molecular imprinted calcium alginate/polyacrylamide hydrogel film for cell adhesion[J]. Chin. Chem. Lett., 2015,26:807-810. doi: 10.1016/j.cclet.2015.04.033
29. [29]
  Wang L., Li L.L., Ma H.L., Wang H.. Recent advances in biocompatible supramolecular assemblies for biomolecular detection and delivery[J]. Chin. Chem. Lett., 2013,24:351-358. doi: 10.1016/j.cclet.2013.03.018
30. [30]
  Zustiak S.P., Durbal R., Leach J.B.. Influence of cell-adhesive peptide ligands on poly(ethylene glycol) hydrogel physical, mechanical and transport properties[J]. Acta Biomater., 2010,6:3404-3414. doi: 10.1016/j.actbio.2010.03.040
31. [31]
  Hern D.L., Hubbell J.A.. Incorporation of adhesion peptides into nonadhesive hydrogels useful for tissue resurfacing[J]. J. Biomed. Mater. Res., 1998,39:266-276. doi: 10.1002/(ISSN)1097-4636
32. [32]
  Ruoslahti E.. Rgd and other recognition sequences for integrins[J]. Annu. Rev. Cell Dev. Biol., 1996,12:697-715. doi: 10.1146/annurev.cellbio.12.1.697
33. [33]
  Chen J.S., Altman G.H., Karageorgiou V.. Human bone marrow stromal cell and ligament fibroblast responses on RGD-modified silk fibers[J]. J. Biomed. Mater. Res., 2003,A 67A:559-570.
34. [34]
  Bi X.D., Luckanagul J.A., Allen A.. Synthesis of PAMAM dendrimer-based fast cross-linking hydrogel for biofabrication[J]. J. Biomater. Sci. Polym. Ed., 2015,26:669-682. doi: 10.1080/09205063.2015.1056716
35. [35]
  Saneinejad S., Shoichet M.S.. Patterned glass surfaces direct cell adhesion and process outgrowth of primary neurons of the central nervous system[J]. J. Biomed. Mater. Res., 1998,42:13-19. doi: 10.1002/(ISSN)1097-4636
36. [36]
  Mann B.K., West J.L.. Cell adhesion peptides alter smooth muscle cell adhesion, proliferation, migration, and matrix protein synthesis on modified surfaces and in polymer scaffolds[J]. J. Biomed. Mater. Res., 2002,60:86-93. doi: 10.1002/(ISSN)1097-4636
37. [37]
  Ranieri J.P., Bellamkonda R., Bekos E.J.. Spatial control of neuronal cell attachment and differentiation on covalently patterned laminin oligopeptide substrates[J]. Int. J. Dev. Neurosci., 1994,12:725-735. doi: 10.1016/0736-5748(94)90052-3
38. [38]
  Bellamkonda R., Ranieri J.P., Aebischer P.. Laminin oligopeptide derivatized agarose gels allow three-dimensional neurite extension in vitro[J]. J. Neurosci. Res., 1995,41:501-509. doi: 10.1002/(ISSN)1097-4547
39. [39]
  Gunn J.W., Turner S.D., Mann B.K.. Adhesive and mechanical properties of hydrogels influence neurite extension[J]. J. Biomed. Mater. Res., A, 2005,72:91-97.
40. [40]
  Johnson S., Nguyen V., Coder D.. Assessment of cell viability, in:Current Protocols in Cytometry, John Wiley & Sons[J]. Inc., New York, NY, 2001.
41. [41]
  Gatti R., Belletti S., Orlandini G.. Comparison of annexin V and calcein-AM as early vital markers of apoptosis in adherent cells by confocal laser microscopy[J]. J. Histochem. Cytochem., 1998,46:895-900. doi: 10.1177/002215549804600804
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