Citation: Ting Wu, Xiao-Hui Yang, Chuan-Jing Zhang, Zhen-Ping Wang, Yi-Ping Du. Label-free quantification of peptides in solution by disposable patterned hydrophilic chip based MALDI imaging[J]. Chinese Chemical Letters, ;2016, 27(6): 901-904. doi: 10.1016/j.cclet.2016.02.020 shu

Label-free quantification of peptides in solution by disposable patterned hydrophilic chip based MALDI imaging

Shanghai Key Laboratory of Functional Materials Chemistry, Research Centre of Analysis and Test, East China University of Science and Technology, Shanghai 200237, China

Received Date: 14 December 2015
Revised Date: 22 January 2016

Fund Project: The authors acknowledge Dr. Yaxin Peng for the assistance on data interpretation. This study was financially supported by the National Natural Science Foundation of China (No. 21205041), the Fundamental Research Funds for the Central Universities (No. 222201314039), and a grant from Shanghai Municipal Education Committee (No. YJ0114209).

Quantification of a mixture of peptides in solution was achieved by disposable patterned hydrophilic chip based matrix-assisted laser desorption/ionization mass spectrometric imaging (MALDI MSI). Compared with other quantitative methods for peptides in solution, this method is label-free and does not require separation of the multiple components of the solution before analysis. Uniform hydrophilic spots and high mass accuracy measurements provided confident identification and quantitative analysis of imaged compounds. The linear correlation between concentration and grayscale of image in the range of 5 fmol/mL to 1 pmol/mL was obtained for all four peptides. Good sensitivity and excellent reproducibility were also achieved. The method expands the application of MALDI MSI from tissues to solutions.
- MALDI imaging,
- Quantitative analysis,
- Label-free,
- Microarray,
- Patterned hydrophilic chip
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