Citation: Yang Shu, Ying Meng, Ming-Li Chen, Jian-Hua Wang. Isolation of hemoglobin with metal-organic frameworks Y(BTC)(H₂O)₆[J]. Chinese Chemical Letters, ;2015, 26(12): 1460-1464. doi: 10.1016/j.cclet.2015.10.013 shu

Isolation of hemoglobin with metal-organic frameworks Y(BTC)(H₂O)₆

Yang Shu ^a,b ,
Ying Meng ^a ,
Ming-Li Chen ^a,, ,
Jian-Hua Wang ^a,,

1.
a Research Center for Analytical Sciences, College of Sciences, Northeastern University, Box 332, Shenyang 110819, China;
2.
b Institute of Biological Technology, College of Life and Health Sciences, Northeastern University, Shenyang 110169, China

Corresponding author: Ming-Li Chen, Jian-Hua Wang,
Received Date: 29 July 2015
Available Online: 21 October 2015

The hierarchical metal-organic frameworks (MOFs), such as Y(BTC)(H₂O)₆, are prepared with yttrium nitrate and benzene-1,3,5-tricarboxylic acid at room temperature. The product is characterized by Fourier transform infrared (FT-IR), X-ray diffraction (XRD), scanning electron microscopy (SEM) and thermogravimetric analysis (TGA). The Y(BTC)(H₂O)₆ particles are sufficiently rigid for performing solid phase extraction and they exhibit favorable selectivity toward the adsorption of hemoglobin. The adsorption behavior of hemoglobin onto the Y(BTC)(H₂O)₆ fits the Langmuir adsorption model with a theoretical adsorption capacity of 555.6 mg g^-1 . An adsorption efficiency of 87.7% for 100 μgmL^-1 hemoglobin in 1 mL sample solution (at pH 6.0) is achieved with 0.40 mg Y(BTC)(H₂O)₆. 77.3% of the retained hemoglobin is readily recovered using a 0.5% (m/v) SDS solution as the stripping reagent. Circular dichroism spectra indicated that the conformation of hemoglobin is maintained during the adsorption-desorption process. The MOFs material is applied for the isolation of hemoglobin from human blood and the purity of the obtained hemoglobin is further verified by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE).
- Metal-organic frameworks
1. [1]
  [1] Q.L. Zhu, Q. Xu, Metal-organic framework composites, Chem. Soc. Rev. 43 (2014) 5468-5512.
2. [2]
  [2] K. Yusuf, A. Aqel, Z. Alothman, Metal-organic frameworks in chromatography, J. Chromatogr. A 1348 (2014) 1-16.
3. [3]
  [3] J.P. Lei, R.C. Qian, P.H. Ling, L. Cui, H.X. Ju, Design and sensing applications of metal-organic framework composites, TrAC Trends Anal. Chem. 58 (2014) 71-78.
4. [4]
  [4] Z.Y. Gu, J. Park, A. Raiff, Z.W. Wei, H.C. Zhou, Metal-organic frameworks as biomimetic catalysts, ChemCatChem 6 (2014) 67-75.
5. [5]
  [5] N.C. Burtch, H. Jasuja, K.S. Walton, Water stability and adsorption in metal- organic frameworks, Chem. Rev. 114 (2014) 10575-10612.
6. [6]
  [6] Z.Y. Gu, Y.J. Chen, J.Q. Jiang, X.P. Yan, Metal-organic frameworks for efficient enrichment of peptides with simultaneous exclusion of proteins from complex biological samples, Chem. Commun. 47 (2011) 4787-4789.
7. [7]
  [7] C.B. Messner, M.R. Mirza, M. Rainer, et al., Selective enrichment of phosphopeptides by a metal-organic framework, Anal. Methods 5 (2013) 2379-2383.
8. [8]
  [8] Y.W. Zhang, Z. Li, Q. Zhao, et al., A facilely synthesized amino-functionalized metal-organic framework for highly specific and efficient enrichment of glycopeptides, Chem. Commun. 50 (2014) 11504-11506.
9. [9]
  [9] M. Zhao, C.H. Deng, X.M. Zhang, P.Y. Yang, Facile synthesis of magnetic metal organic frameworks for the enrichment of low-abundance peptides for MALDI- TOF MS analysis, Proteomics 13 (2013) 3387-3392.
10. [10]
  [10] J.N. Zheng, Z. Lin, G. Lin, H.H. Yang, L. Zhang, Preparation of magnetic metal- organic framework nanocomposites for highly specific separation of histidinerich proteins, J. Mater. Chem. B 3 (2015) 2185-2191.
11. [11]
  [11] J.W. Liu, Y. Zhang, X.W. Chen, J.H. Wang, Graphene oxide-rare earth metal- organic framework composites for the selective isolation of hemoglobin, ACS Appl. Mater. Interfaces 6 (2014) 10196-10204.
12. [12]
  [12] S.Z. Li, F.W. Huo, Metal-organic framework composites: from fundamentals to applications, Nanoscale 7 (2015) 7482-7501.
13. [13]
  [13] T.W. Duan, B. Yan, Hybrids based on lanthanide ions activated yttrium metal- organic frameworks: functional assembly, polymer film preparation and luminescence tuning, J. Mater. Chem. C 2 (2014) 5098-5104.
14. [14]
  [14] F. Wang, K.J. Deng, G.L. Wu, et al., Facile and large-scale syntheses of nanocrystal rare earth metal-organic frameworks at room temperature and their photoluminescence properties, J. Inorg. Organomet. Polym. 22 (2012) 680-685.
15. [15]
  [15] Z. Rzaczyń ska, A. Ostasz, S. Pikus, Thermal properties of rare earth elements complexes with 1,3,5-benzenetricarboxylic acid, J. Therm. Anal. Calorim. 82 (2005) 347-351.
16. [16]
  [16] K. Nakamoto, Infrared and Raman Spectra of Inorganic Coordination Compounds, John Wiley, New York, NY, 2006.
17. [17]
  [17] Y.H. Wen, J.K. Cheng, Y.L. Feng, et al., Synthesis and crystal structure of [La(BTC)(H₂O)₆]_n, Chin. J. Struct. Chem. 24 (2005) 1440-1444.
18. [18]
  [18] J.H. Luo, H.W. Xu, Y. Liu, et al., Hydrogen adsorption in a highly stable porous rareearth metal-organic framework: sorption properties and neutron diffraction studies, J. Am. Chem. Soc. 130 (2008) 9626-9627.
19. [19]
  [19] J. Rouquerol, F. Rouquerol, K.S.W. Sing, Adsorption by Powders and Porous Solids: Principles, Methodology and Applications, Academic Press, San Diego, CA, 1998.
20. [20]
  [20] Y. Shu, X.W. Chen, J.H. Wang, Ionic liquid-polyvinyl chloride ionomer for highly selective isolation of basic proteins, Talanta 81 (2010) 637-642.
1. [1]
  Muhammad Riaz , Rakesh Kumar Gupta , Di Sun , Mohammad Azam , Ping Cui . Selective adsorption of organic dyes and iodine by a two-dimensional cobalt(II) metal-organic framework. Chinese Journal of Structural Chemistry, 2024, 43(12): 100427-100427. doi: 10.1016/j.cjsc.2024.100427
2. [2]
  Tengjia Ni , Xianbiao Hou , Huanlei Wang , Lei Chu , Shuixing Dai , Minghua Huang . Controllable defect engineering based on cobalt metal-organic framework for boosting oxygen evolution reaction. Chinese Journal of Structural Chemistry, 2024, 43(1): 100210-100210. doi: 10.1016/j.cjsc.2023.100210
3. [3]
  Xi Feng , Ding-Yi Hu , Zi-Jun Liang , Mu-Yang Zhou , Zhi-Shuo Wang , Wen-Yu Su , Rui-Biao Lin , Dong-Dong Zhou , Jie-Peng Zhang . A metal azolate framework with small aperture for highly efficient ternary benzene/cyclohexene/cyclohexane separation. Chinese Journal of Structural Chemistry, 2025, 44(3): 100540-100540. doi: 10.1016/j.cjsc.2025.100540
4. [4]
  Jiayu Huang , Kuan Chang , Qi Liu , Yameng Xie , Zhijia Song , Zhiping Zheng , Qin Kuang . Fe-N-C nanostick derived from 1D Fe-ZIFs for Electrocatalytic oxygen reduction. Chinese Journal of Structural Chemistry, 2023, 42(10): 100097-100097. doi: 10.1016/j.cjsc.2023.100097
5. [5]
  Ze Liu , Xiaochen Zhang , Jinlong Luo , Yingjian Yu . Application of metal-organic frameworks to the anode interface in metal batteries. Chinese Chemical Letters, 2024, 35(11): 109500-. doi: 10.1016/j.cclet.2024.109500
6. [6]
  Longlong Geng , Huiling Liu , Wenfeng Zhou , Yong-Zheng Zhang , Hongliang Huang , Da-Shuai Zhang , Hui Hu , Chao Lv , Xiuling Zhang , Suijun Liu . Construction of metal-organic frameworks with unsaturated Cu sites for efficient and fast reduction of nitroaromatics: A combined experimental and theoretical study. Chinese Chemical Letters, 2024, 35(8): 109120-. doi: 10.1016/j.cclet.2023.109120
7. [7]
  Rui Wang , He Qi , Haijiao Zheng , Qiong Jia . Light/pH dual-responsive magnetic metal-organic frameworks composites for phosphorylated peptide enrichment. Chinese Chemical Letters, 2024, 35(7): 109215-. doi: 10.1016/j.cclet.2023.109215
8. [8]
  Fereshte Hassanzadeh-Afruzi , Mina Azizi , Iman Zare , Ehsan Nazarzadeh Zare , Anwarul Hasan , Siavash Iravani , Pooyan Makvandi , Yi Xu . Advanced metal-organic frameworks-polymer platforms for accelerated dermal wound healing. Chinese Chemical Letters, 2024, 35(11): 109564-. doi: 10.1016/j.cclet.2024.109564
9. [9]
  Xinyu Wu , Jianfeng Lu , Zihao Zhu , Suijun Liu , Herui Wen . Recent advances of metal-organic frameworks and MOF-derived materials based on p-block metal for the electrochemical reduction of carbon dioxide. Chinese Chemical Letters, 2025, 36(7): 110151-. doi: 10.1016/j.cclet.2024.110151
10. [10]
  Xiao-Hong Yi , Chong-Chen Wang . Metal-organic frameworks on 3D interconnected macroporous sponge foams for large-scale water decontamination: A mini review. Chinese Chemical Letters, 2024, 35(5): 109094-. doi: 10.1016/j.cclet.2023.109094
11. [11]
  Fahui Xiang , Lu Li , Zhen Yuan , Wuji Wei , Xiaoqing Zheng , Shimin Chen , Yisi Yang , Liangji Chen , Zizhu Yao , Jianwei Fu , Zhangjing Zhang , Shengchang Xiang . Enhanced C₂H₂/CO₂ separation in tetranuclear Cu(Ⅱ) cluster-based metal-organic frameworks by adjusting divider length of pore space partition. Chinese Chemical Letters, 2025, 36(3): 109672-. doi: 10.1016/j.cclet.2024.109672
12. [12]
  Wenbiao Zhang , Bolong Yang , Zhonghua Xiang . Atomically dispersed Cu-based metal-organic framework directly for alkaline polymer electrolyte fuel cells. Chinese Chemical Letters, 2025, 36(2): 109630-. doi: 10.1016/j.cclet.2024.109630
13. [13]
  Changmin Liu , Ying Wang , Yongqi Bao , Yuqing Lin . Metal-organic framework mimetic enzymes: Exploring new horizons in brain chemistry. Chinese Chemical Letters, 2025, 36(9): 110652-. doi: 10.1016/j.cclet.2024.110652
14. [14]
  Xudong Zhao , Yuxuan Wang , Xinxin Gao , Xinli Gao , Meihua Wang , Hongliang Huang , Baosheng Liu . Anchoring thiol-rich traps in 1D channel wall of metal-organic framework for efficient removal of mercury ions. Chinese Chemical Letters, 2025, 36(2): 109901-. doi: 10.1016/j.cclet.2024.109901
15. [15]
  Sixiao Liu , Tianyi Wang , Lei Zhang , Chengyin Wang , Huan Pang . Cerium-based metal-organic framework-modified natural mineral vermiculite for photocatalytic nitrogen fixation under visible-light irradiation. Chinese Chemical Letters, 2025, 36(3): 110058-. doi: 10.1016/j.cclet.2024.110058
16. [16]
  Zhi-Xin Li , Xiao-Feng Qiu , Pei-Qin Liao . Efficient electroreduction of CO₂ to acetate with relative purity of 100% by ultrasmall Cu₂O nanoparticle on a conductive metal-organic framework. Chinese Chemical Letters, 2025, 36(11): 110473-. doi: 10.1016/j.cclet.2024.110473
17. [17]
  Shan-Qing Yang , Lu-Lu Wang , Rajamani Krishna , Bo Xing , Lei Zhou , Fei-Yang Zhang , Qiang Zhang , Yi-Long Li , Chao-Sheng Bao , Tong-Liang Hu . Efficient C₃H₆/C₃H₈ separation within a bifunctional ultramicroporous metal-organic framework with high purity and record packing density. Chinese Chemical Letters, 2025, 36(12): 110556-. doi: 10.1016/j.cclet.2024.110556
18. [18]
  Xin-Lou Yang , Jieying Hu , Hao Zhong , Qia-Chun Lin , Zhiqing Lin , Lai-Hon Chung , Jun He . Building metal-thiolate sites and forming heterojunction in Hf- and Zr-based thiol-dense frameworks towards stable integrated photocatalyst for hydrogen evolution. Chinese Chemical Letters, 2025, 36(7): 110120-. doi: 10.1016/j.cclet.2024.110120
19. [19]
  Ming Yue , Yi-Rong Wang , Jia-Yong Weng , Jia-Li Zhang , Da-Yu Chi , Mingjin Shi , Xiao-Gang Hu , Yifa Chen , Shun-Li Li , Ya-Qian Lan . Multi-metal porous crystalline materials for electrocatalysis applications. Chinese Chemical Letters, 2025, 36(6): 110049-. doi: 10.1016/j.cclet.2024.110049
20. [20]
  Kunpeng Zhou , Zhihao Shi , Xiao-Hong Yi , Peng Wang , Aiqun Li , Chong-Chen Wang . MOFs helping heritage against environmental threats. Chinese Chemical Letters, 2025, 36(5): 110226-. doi: 10.1016/j.cclet.2024.110226

Get Citation

PDF

XML

Metrics

PDF Downloads(0)
Abstract views(1305)
HTML views(59)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Isolation of hemoglobin with metal-organic frameworks Y(BTC)(H2O)6

Metrics

通讯作者: 陈斌, bchen63@163.com

Isolation of hemoglobin with metal-organic frameworks Y(BTC)(H₂O)₆