Citation: Tian-Tian Meng, Ying-Xin Liu, Meng-Tan Liu, Jiao-Bao Long, Qing-Feng Cao, Shu-Ya Yan, Xiang-Xian Meng. Lineal DNA logic gate for microRNA diagnostics with strand displacement and fluorescence resonance energy transfer[J]. Chinese Chemical Letters, ;2015, 26(9): 1179-1182. doi: 10.1016/j.cclet.2015.05.039 shu

Lineal DNA logic gate for microRNA diagnostics with strand displacement and fluorescence resonance energy transfer

1.
1 College of Biology, The High School Attach to Hunan University, Hunan University, Changsha 410082, China;
2.
2 School of Life Science, Xiamen University, Xiamen 361005, China

Corresponding author: Xiang-Xian Meng,
Received Date: 20 January 2015
Available Online: 21 April 2015
Fund Project: This work is supported by National Natural Science Foundation of China (No. 21275043) (No. 21275043)National Basic Research Program of China under Grants (No. 2009CB421601). (No. 2009CB421601)

Designing molecular logic gates to operate programmably for molecular diagnostics in molecular computing still remains challenging. Here, we designed a novel linear DNA logic gates for microRNA analysis based on strand displacement and fluorescence resonance energy transfer (FRET). Two labeled strands closed each other produce to FRET through hybridization with a complementary strand to form a basic work unit of logic gate. Two indicators of heart failure (microRNA-195 and microRNA-21) were selected as the logic inputs and the fluorescence mode was used as the logic output. We have demonstrated that the molecular logic gate mechanism worked well with the construction of YES and AND gates.
- Molecular logic gate,
- Strand displacement,
- Fluorescence resonance energy transfer
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