Citation: Hai-Yan Tian, Peng-Wei Zhang, Jun-Shan Liu, Dong-Mei Zhang, Xiao-Qi Zhang, Ren-Wang Jiang, Wen-Cai Ye. New cytotoxic C-3 dehydrated bufadienolides from the venom of Bufo bufo gargarizans[J]. Chinese Chemical Letters, ;2014, 25(7): 1104-1106. doi: 10.1016/j.cclet.2014.02.006 shu

New cytotoxic C-3 dehydrated bufadienolides from the venom of Bufo bufo gargarizans

1.
a Institute of Traditional Chinese Medicine and Natural Products, College of Pharmacy, Ji'nan University, Guangzhou 510632, China;
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b JNU-HKUST Joint Laboratory for Neuroscience & Innovative Drug Research, Ji'nan University, Guangzhou 510632, China;
3.
c School of Traditional Chinese Medicine, Southern Medical University, Southern Medical University, Guangzhou 510515, China

Corresponding author: Ren-Wang Jiang, Wen-Cai Ye,
Received Date: 31 December 2013
Available Online: 13 February 2014
Fund Project: This work was supported by National Natural Science Foundation of China (No. 81102518) (No. 81102518)the Guangdong High Level Talent Scheme (R.W.J.). (No. 20110490915)

Three new C-3 dehydrated bufadienolides were isolated from the venom of Bufo bufo gargarizans. Their structures were elucidated as 5β,12β-12,14-dihydroxy-11-oxobufa-3,20,22-trienolide (1), 5β,12α-12,14-dihydroxy-11-oxobufa-2,20,22-trienolide (2), and 5β,12β-12,14-dihydroxy-11-oxobufa-2,20,22-trienolide (3) on the basis of extensive spectroscopic analysis, especially 1D NMR and 2D NMR data. In addition, all three compounds were tested for their cytotoxic activities against A549 and HepG2 cancer cell lines. Compounds 2 and 3 showed significant cytotoxicities with IC₅₀ values less than 10 μmol/L on both cancer cells.
- Toad venom,
- Bufo bufo gargarizans,
- 3-Dehydrated bufadienolide,
- Cytotoxicity
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