Citation: Yu Cai He, Jian He Xu, Yi Xu, Li Ming Ouyang, Jiang Pan. Biocatalytic synthesis of (R)-(-)-mandelic acid from racemic mandelonitrile by a newly isolated nitrilase-producer Alcaligenes sp. ECU0401[J]. Chinese Chemical Letters, ;2007, 18(6): 677-680. doi: 10.1016/j.cclet.2007.04.034 shu

Biocatalytic synthesis of (R)-(-)-mandelic acid from racemic mandelonitrile by a newly isolated nitrilase-producer Alcaligenes sp. ECU0401

Laboratory of Biocatalysis and Bioprocessing, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China

Fund Project: This work was financially supported by the National Natural Science Foundation of China (Nos. 20506037 and 20672037).

By using acetonitrile as the sole nitrogen source, a microbial strain with high nitrilase activity, named as Alcaligenes sp.ECU0401, was newly isolated from soil, which could enantioselectively transform racemic mandelonitrile into (R)-(-)-mandelic acid, with an enantiomeric excess of >99.9%.
- Mandelonitrile,
- Nitrilase,
- (R)-(-)-Mandelic acid,
- Screening,
- Alcaligenes sp. ECU0401
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