引用本文:
Hong Wang, Xue Jiang Wang, Jian Fu Zhao, Ling Chen. Toxicity assessment of heavy metals and organic compounds using CellSense biosensor with E.coli[J]. Chinese Chemical Letters,
2008, 19(2): 211-214.
doi:
10.1016/j.cclet.2007.10.053
Citation: Hong Wang, Xue Jiang Wang, Jian Fu Zhao, Ling Chen. Toxicity assessment of heavy metals and organic compounds using CellSense biosensor with E.coli[J]. Chinese Chemical Letters, 2008, 19(2): 211-214. doi: 10.1016/j.cclet.2007.10.053
Citation: Hong Wang, Xue Jiang Wang, Jian Fu Zhao, Ling Chen. Toxicity assessment of heavy metals and organic compounds using CellSense biosensor with E.coli[J]. Chinese Chemical Letters, 2008, 19(2): 211-214. doi: 10.1016/j.cclet.2007.10.053
Toxicity assessment of heavy metals and organic compounds using CellSense biosensor with E.coli
摘要:
A new strategy using an amperometric biosensor with Escherichia coli(E.coli) that provides a rapid toxicity determination of chemical compounds is described.The CellSense biosensor system comprises a biological component immobilized in intimate contact with a transducer which converts the biochemical signal into a quantifiable electrical signal.Toxicity assessment of heavy metals using E.coli biosensors could be finished within 30 min and the 50% effective concentrations (EC50) values of four heavy metals were determined.The results shows that inhibitory effects of four heavy metals to E.coli can be ranked in a decreasing order of Hg2+ > Cu2+ > Zn2+ > Ni2+, which accords to the results of conventional bacterial counting method.The toxicity test of organic compounds by using CellSense biosensor was also demonstrated.The CellSense biosensor with E.coli shows a good, reproducible behavior and can be used for reproducible measurements.
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关键词:
- Whole-cell biosensor
- / Heavy metal
- / Organic compounds
- / Toxicity
English
Toxicity assessment of heavy metals and organic compounds using CellSense biosensor with E.coli
Abstract:
A new strategy using an amperometric biosensor with Escherichia coli(E.coli) that provides a rapid toxicity determination of chemical compounds is described.The CellSense biosensor system comprises a biological component immobilized in intimate contact with a transducer which converts the biochemical signal into a quantifiable electrical signal.Toxicity assessment of heavy metals using E.coli biosensors could be finished within 30 min and the 50% effective concentrations (EC50) values of four heavy metals were determined.The results shows that inhibitory effects of four heavy metals to E.coli can be ranked in a decreasing order of Hg2+ > Cu2+ > Zn2+ > Ni2+, which accords to the results of conventional bacterial counting method.The toxicity test of organic compounds by using CellSense biosensor was also demonstrated.The CellSense biosensor with E.coli shows a good, reproducible behavior and can be used for reproducible measurements.
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Key words:
- Whole-cell biosensor
- / Heavy metal
- / Organic compounds
- / Toxicity
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