引用本文:
Jun GAO, Yuan Zong LI, Zhen Quan GUO, Wen Bac CHANG, Yun Xiang CI. Poly-N-Isopropylacrylamide Hydrogel Based Fluoroimmunoassay of Methyltestosterone[J]. Chinese Chemical Letters,
1999, 10(6): 495-498.
Citation: Jun GAO, Yuan Zong LI, Zhen Quan GUO, Wen Bac CHANG, Yun Xiang CI. Poly-N-Isopropylacrylamide Hydrogel Based Fluoroimmunoassay of Methyltestosterone[J]. Chinese Chemical Letters, 1999, 10(6): 495-498.
Citation: Jun GAO, Yuan Zong LI, Zhen Quan GUO, Wen Bac CHANG, Yun Xiang CI. Poly-N-Isopropylacrylamide Hydrogel Based Fluoroimmunoassay of Methyltestosterone[J]. Chinese Chemical Letters, 1999, 10(6): 495-498.
Poly-N-Isopropylacrylamide Hydrogel Based Fluoroimmunoassay of Methyltestosterone
摘要:
A novel immunoassay method for methyltestosterone (MT) in serum was developed. The antigen of MT (AgMT)was synthesized by covalently bonding MT to BSA and raising its antibody (AbMT). AbMT was conjugated to poly-N-isopropylacrylamide (p-NIPAAm) to form AbMT bound thermally reversible hydrogel p-NIPAAm-AbMT. A competitive immunoassay method based on the competition of fluorescein isothiocyanate (FITC) labeled MT antigen (FITC-AgMT) and free MT with limited amount of p-NIPAAm-AbMT was established. The separation in the assay process was achieved by precipitation of the immuno-complex above its critical solution temperature. The detection limit for MT is 50 ng/ml. The recoveries of MT from human serum are satisfactory.
English
Poly-N-Isopropylacrylamide Hydrogel Based Fluoroimmunoassay of Methyltestosterone
Abstract:
A novel immunoassay method for methyltestosterone (MT) in serum was developed. The antigen of MT (AgMT)was synthesized by covalently bonding MT to BSA and raising its antibody (AbMT). AbMT was conjugated to poly-N-isopropylacrylamide (p-NIPAAm) to form AbMT bound thermally reversible hydrogel p-NIPAAm-AbMT. A competitive immunoassay method based on the competition of fluorescein isothiocyanate (FITC) labeled MT antigen (FITC-AgMT) and free MT with limited amount of p-NIPAAm-AbMT was established. The separation in the assay process was achieved by precipitation of the immuno-complex above its critical solution temperature. The detection limit for MT is 50 ng/ml. The recoveries of MT from human serum are satisfactory.
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