引用本文:
Xiang Yi HUANG, Jiao Ning WANG, Lin CHEN, Ji Cun REN. Highly Sensitive Chemiluminescence Detection for PDMS/Glass Micro-chip Electrophoresis[J]. Chinese Chemical Letters,
2004, 15(6): 683-686.
Citation: Xiang Yi HUANG, Jiao Ning WANG, Lin CHEN, Ji Cun REN. Highly Sensitive Chemiluminescence Detection for PDMS/Glass Micro-chip Electrophoresis[J]. Chinese Chemical Letters, 2004, 15(6): 683-686.
Citation: Xiang Yi HUANG, Jiao Ning WANG, Lin CHEN, Ji Cun REN. Highly Sensitive Chemiluminescence Detection for PDMS/Glass Micro-chip Electrophoresis[J]. Chinese Chemical Letters, 2004, 15(6): 683-686.
Highly Sensitive Chemiluminescence Detection for PDMS/Glass Micro-chip Electrophoresis
摘要:
This paper described a highly sensitive chemiluminescence detection system for micro-chip electrophoresis (MCE) based on luminol-hydrogen peroxide reaction catalyzed by the metal ions. The micro-chip was composed of poly(dimethylsiloxane) (PDMS) and glass, and was fabricated by micro-machining technology. The surface of channels was dynamically modified by polydimethylacrylamide (PDMA) in order to eliminate unhomogeneous electroosmotic flow (EOF) of the PDMS/glass chip, adsorption of molecules, and improve hydrophobicity on PDMS surface. The detection modes, reagent mix procedures and reaction conditions were optimized and the detection limit of 5 x 10-11 mol/L for cobalt (II) was achieved by MCE with chemiluminescence detection, which was about four orders of magnitude more sensitive than that reported in the reference.
English
Highly Sensitive Chemiluminescence Detection for PDMS/Glass Micro-chip Electrophoresis
Abstract:
This paper described a highly sensitive chemiluminescence detection system for micro-chip electrophoresis (MCE) based on luminol-hydrogen peroxide reaction catalyzed by the metal ions. The micro-chip was composed of poly(dimethylsiloxane) (PDMS) and glass, and was fabricated by micro-machining technology. The surface of channels was dynamically modified by polydimethylacrylamide (PDMA) in order to eliminate unhomogeneous electroosmotic flow (EOF) of the PDMS/glass chip, adsorption of molecules, and improve hydrophobicity on PDMS surface. The detection modes, reagent mix procedures and reaction conditions were optimized and the detection limit of 5 x 10-11 mol/L for cobalt (II) was achieved by MCE with chemiluminescence detection, which was about four orders of magnitude more sensitive than that reported in the reference.
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Key words:
- Micro-chip
- / electrophoresis
- / chemiluminenscence
- / poly(dimethylsiloxane)
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