Citation: Wei SUN, Kui JIAO, Xue Liang WANG, Lu De LU. Studies on the Interaction of Beryllon Ш with Proteins by Voltammetric Technique and its Application[J]. Chinese Chemical Letters, ;2003, 14(12): 1275-1277. shu

Studies on the Interaction of Beryllon Ш with Proteins by Voltammetric Technique and its Application

Wei SUN ^1,2, ,
Kui JIAO ² ,
Xue Liang WANG ² ,
Lu De LU ¹

1.
College of Chemical Engineering, Nanjing University of Science and Technology, Nanjing 210094;
2.
College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042

Received Date: 15 November 2002

Fund Project: The work was supported by the National Natural Science Foundation of China (Grant No. 20075013) and the Younger Foundation of Qingdao University of Science and Technology (Grant No. 22000219).

A new quantitative determination method of proteins using beryllon Ш by voltammetric technique was developed in this paper. In pH 3.5 Britton-Robinson (B-R) buffer solution, beryllon Ш can bind with human serum albumin (HSA) to form an electro-inactive supermolecular complex. Beryllon Ш has a well-defined voltammetric reduction peak at -0.38 V (vs. SCE) and the addition of protein in this solution can cause the decrease of the reductive peak current. Based on the decrease of the reduction peak current, a new electrochemical method for the determination of HSA was established with linear range of 1.0~40.0 mg/L and the detection limit of 1.0 mg/L. This method is further applied to the determination of real sample of healthy human serum.
- Beryllon Ш,
- serum albumin,
- voltammetry,
- binding reaction
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