Citation: Li GUO, Zi Min LU, Heiner ECKSTEIN. Enzymatic Synthesis of a CCK-8 Tripeptide Derivative[J]. Chinese Chemical Letters, ;2003, 14(2): 167-168. shu

Enzymatic Synthesis of a CCK-8 Tripeptide Derivative

1.
West China School of Pharmacy, Sichuan University, Chengdu 610041;
2.
Tongji Medical College, Huazhong Science & Technology University, Wuhan 430030;
3.
Institute of Organic Chemistry, University of Tuebingen, 72076 Tuebingen, Germany

Received Date: 20 May 2002

The enzymatic synthesis of CCK-8 tripeptide derivative Phac-Met-Asp(OMe)-Phe-NH₂ is reported.Starting with Phac-Met-OCam, we have successfully synthesized the target tripeptide with three free or immobilized enzymes, α-chymotrypsin, papain and thermolysin in reasonable yields.The key steps in this synthesis were the coupling of Phac-Met-OCam and H-Asp(OMe)₂ to form Met-Asp peptide bond catalyzed by α-chymotrypsin and the selective hydrolysis of α-ester of Phac-Met-Asp(OMe)₂ catalyzed by papain.
- Enzymatic synthesis,
- CCK-8,
- tripeptide derivative
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