Citation: Ji ZHANG, Chun Sheng LIU, Yi Zhong ZHANG, Chang Jiu ZHANG, Li YE, Tong Hui ZHOU. GAS CHROMATOGRAPHIC-MASS SPECTROMETRIC ANALYSIS OF ANABOLIC STEROIDS IN HUMAN URINE[J]. Chinese Chemical Letters, ;1990, 1(2): 143-146. shu

GAS CHROMATOGRAPHIC-MASS SPECTROMETRIC ANALYSIS OF ANABOLIC STEROIDS IN HUMAN URINE

a Institute of Materia Medica, 1 Xian Nong Tan Street, Beijing 100050, P.R.China;
b National Research Institute of Sports Medicine, 11 Tiyuguan Road, Beijing 100061

Received Date: 15 December 1989

A new GC/MS method for detection and identification of 19 anabolic steroids in human urine is presented.The procedure involves adsorption and isolation on a macroporous XAD-2 resin,enzymatic hydrolysis,alkaline extraction,derivatization,GC separation and MS detection.Gas chromatographic-mass spectrometric data illustrate artifacts arising from enzymatic hydrolysis of steroid glucuronides and the structural characterization of their metabolites.Using this method,metabolic studies of these steroids in human urine were made after their ingestion by normal and healthy male volunteers.This method was proven to be suitable for large-scale routine analysis of anabolic steroids and was used successfully in passing the doping control test held by the Medical Commission of the International Olympic Committee.
1. [1]
  Cheng Guo , Xiaoxiao Zhang , Xiujuan Hong , Yiqiu Hu , Lingna Mao , Kezhi Jiang . Graphene as adsorbent for highly efficient extraction of modified nucleosides in urine prior to liquid chromatography-tandem mass spectrometry analysis. Chinese Chemical Letters, 2024, 35(4): 108867-. doi: 10.1016/j.cclet.2023.108867
2. [2]
  Haoyang Wang , Ronghao Zhang , Yanlun Ren , Li Zhang . A convenient method for measuring gas-liquid volumetric mass transfer coefficient in micro reactors. Chinese Chemical Letters, 2024, 35(4): 108833-. doi: 10.1016/j.cclet.2023.108833
3. [3]
  Tian Feng , Yun-Ling Gao , Di Hu , Ke-Yu Yuan , Shu-Yi Gu , Yao-Hua Gu , Si-Yu Yu , Jun Xiong , Yu-Qi Feng , Jie Wang , Bi-Feng Yuan . Chronic sleep deprivation induces alterations in DNA and RNA modifications by liquid chromatography-mass spectrometry analysis. Chinese Chemical Letters, 2024, 35(8): 109259-. doi: 10.1016/j.cclet.2023.109259
4. [4]
  Keqiang Shi , Xiujuan Hong , Dongyan Xu , Tao Pan , Huiwen Wang , Hongru Feng , Cheng Guo , Yuanjiang Pan . Analysis of RNA modifications in peripheral white blood cells from breast cancer patients by mass spectrometry. Chinese Chemical Letters, 2025, 36(3): 110079-. doi: 10.1016/j.cclet.2024.110079
5. [5]
  Tian Feng , Shu-Yi Gu , Lu-Fei Shi , Yao-Hua Gu , Bi-Feng Yuan . Mass spectrometry analysis reveals changes in patterns and correlations of nucleic acid epigenetic modifications in mouse tissues exposed to polystyrene microplastics. Chinese Chemical Letters, 2026, 37(3): 111546-. doi: 10.1016/j.cclet.2025.111546
6. [6]
  Genlin Sun , Yachun Luo , Zhihong Yan , Hongdeng Qiu , Weiyang Tang . Chiral metal-organic frameworks-based materials for chromatographic enantioseparation. Chinese Chemical Letters, 2024, 35(12): 109787-. doi: 10.1016/j.cclet.2024.109787
7. [7]
  Quan Zhang , Shunjie Xing , Jingqian Han , Li Feng , Jianchun Li , Zhaosheng Qian , Jin Zhou . Organic pollutant sensing for human health based on carbon dots. Chinese Chemical Letters, 2025, 36(1): 110117-. doi: 10.1016/j.cclet.2024.110117
8. [8]
  Ting Li , Xinxin Zheng , Lejing Qu , Yuanyuan Ou , Sai Qiao , Xue Zhao , Yajun Zhang , Xinfeng Zhao , Qian Li . A chromatographic method for pursuing potential GPCR ligands with the capacity to characterize their intrinsic activities of regulating downstream signaling pathway. Chinese Chemical Letters, 2024, 35(10): 109792-. doi: 10.1016/j.cclet.2024.109792
9. [9]
  Min Huang , Ru Cheng , Shuai Wen , Liangtong Li , Jie Gao , Xiaohui Zhao , Chunmei Li , Hongyan Zou , Jian Wang . Ultrasensitive detection of microRNA-21 in human serum based on the confinement effect enhanced chemical etching of gold nanorods. Chinese Chemical Letters, 2024, 35(9): 109379-. doi: 10.1016/j.cclet.2023.109379
10. [10]
  Xiujuan Qiao , Rui Han , Xinru Xu , Mingrui Lv , Yiting Hou , Xiliang Luo . A robust nonfouling electrochemical biosensor with both filtering and antifouling strategies for cortisol detection in human blood. Chinese Chemical Letters, 2026, 37(3): 111598-. doi: 10.1016/j.cclet.2025.111598
11. [11]
  Qiuting Zhang , Fan Wu , Jin Liu , Hang Su , Yanhui Zhong , Zian Lin . Facile synthesis of single-crystal 3D covalent organic frameworks as stationary phases for high-performance liquid chromatographic separation. Chinese Chemical Letters, 2025, 36(8): 110649-. doi: 10.1016/j.cclet.2024.110649
12. [12]
  Xueling Yu , Lixing Fu , Tong Wang , Zhixin Liu , Na Niu , Ligang Chen . Multivariate chemical analysis: From sensors to sensor arrays. Chinese Chemical Letters, 2024, 35(7): 109167-. doi: 10.1016/j.cclet.2023.109167
13. [13]
  Weifei Zhang , Danfeng Xu , Jinru Tian , Ying Kan , Xuanlin Feng , Jia Liu , Hongmei Li , Nan Li . An inverse opal structure-based human alveolar lung-on-a-chip for the study of Osimertinib treatment in non-small cell lung cancer. Chinese Chemical Letters, 2026, 37(4): 111453-. doi: 10.1016/j.cclet.2025.111453
14. [14]
  Xiaoyu Du , Huan Wang . Tailoring mass transfer on electrochemical fixation of air-abundant molecules. Chinese Chemical Letters, 2025, 36(8): 110276-. doi: 10.1016/j.cclet.2024.110276
15. [15]
  Xia Chen , Ting Dai , Meng-Ying Yin , Xing-Yuan Xia , Qiu-Ju Xing , Lei Tian , Jian-Ping Zou . Enhanced anodic mass transfer enables interfacial Cl^• for efficient ammonia oxidation. Chinese Chemical Letters, 2026, 37(3): 111445-. doi: 10.1016/j.cclet.2025.111445
16. [16]
  Xiaoyan Peng , Xuanhao Wu , Fan Yang , Yefei Tian , Mingming Zhang , Hongye Yuan . Gas sensors based on metal-organic frameworks: challenges and opportunities. Chinese Journal of Structural Chemistry, 2024, 43(3): 100251-100251. doi: 10.1016/j.cjsc.2024.100251
17. [17]
  Hongxia Li , Xiyang Wang , Du Qiao , Jiahao Li , Weiping Zhu , Honglin Li . Mechanism of nanoparticle aggregation in gas-liquid microfluidic mixing. Chinese Chemical Letters, 2024, 35(4): 108747-. doi: 10.1016/j.cclet.2023.108747
18. [18]
  Jie Ma , Jianxiang Wang , Jianhua Yuan , Xiao Liu , Yun Yang , Fei Yu . The regulating strategy of hierarchical structure and acidity in zeolites and application of gas adsorption: A review. Chinese Chemical Letters, 2024, 35(11): 109693-. doi: 10.1016/j.cclet.2024.109693
19. [19]
  Neng Shi , Haonan Jia , Jixiang Zhang , Pengyu Lu , Chenglong Cai , Yixin Zhang , Liqiang Zhang , Nongyue He , Weiran Zhu , Yan Cai , Zhangqi Feng , Ting Wang . Accurate expression of neck motion signal by piezoelectric sensor data analysis. Chinese Chemical Letters, 2024, 35(9): 109302-. doi: 10.1016/j.cclet.2023.109302
20. [20]
  Yuxin Li , Chengbin Liu , Qiuju Li , Shun Mao . Fluorescence analysis of antibiotics and antibiotic-resistance genes in the environment: A mini review. Chinese Chemical Letters, 2024, 35(10): 109541-. doi: 10.1016/j.cclet.2024.109541

Get Citation

PDF

XML

Metrics

PDF Downloads(1)
Abstract views(1454)
HTML views(29)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Address：Zhongguancun North First Street 2,100190 Beijing, PR China Tel: +86-010-82449177-888

DownLoad: Full-Size Img PowerPoint

Return