Citation: Li SHEN, Bo Hui XIONG, Run Zhi CONG, Jun De WANG. Preparation of Cibacron Blue F3GA Bonded Poly (styrenedivinylbenzene) (PSDVB) Microbeads Used for High Performance Affinity Chromatography[J]. Chinese Chemical Letters, ;1999, 10(7): 583-586. shu

Preparation of Cibacron Blue F3GA Bonded Poly (styrenedivinylbenzene) (PSDVB) Microbeads Used for High Performance Affinity Chromatography

Dalian Institute of Chemical Physics, The Chinese Academy of Sciences, Dalian 116012

Received Date: 1 January 1999

Fund Project: This work is supported by the National Natural Science Fundation of China.

Rigid PSDVB microbeads have been modified with poly（vinyl alcohol) (PVA) adsorbed on their surface to produce an affinny medium. Then Cibacron Blue F3GA was covalentlv attached to the supports. The initial concentration of PVA has effect on the adsorption of PVA. The non-specific interaction of bovine serum albumin (BSA) on the microbeads decreases、、ith the PVA adsorbed. The pH stability test shows that the aftinitv medium is stable up to pH 11.0. And it has specific interaction with lysoryme, but not with pepsin.
- PSDVB microbeads,
- PVA,
- affinity medium,
- Cibacron Blue F3GA
1. [1]
  Xing Cao , Xinyu Tian , Yuanyuan Huang , Liping Zhang , Yanpeng Ni , Yu-Zhong Wang . H₃PO₃-protonated chitosan enabling flame-retardant and antibacterial PVA composite films with high strength and toughness through multiple H-bonds and interlocking interfaces. Chinese Chemical Letters, 2025, 36(11): 111382-. doi: 10.1016/j.cclet.2025.111382
2. [2]
  Jiajia Lv , Jie Gao , Hongyu Li , Zeli Yuan , Nan Dong . Rational design of hydroxytricyanopyrrole-based probes with high affinity and rapid visualization for amyloid-β aggregates in vitro and in vivo. Chinese Chemical Letters, 2024, 35(5): 108940-. doi: 10.1016/j.cclet.2023.108940
3. [3]
  Xu-Hui Yue , Xiang-Wen Zhang , Hui-Min He , Lei Qiao , Zhong-Ming Sun . Synthesis, chemical bonding and reactivity of new medium-sized polyarsenides. Chinese Chemical Letters, 2024, 35(7): 108907-. doi: 10.1016/j.cclet.2023.108907
4. [4]
  Ping Liu , Fei Yu . Covalent organic framework ionomers for medium-temperature fuel cells. Chinese Journal of Structural Chemistry, 2025, 44(4): 100465-100465. doi: 10.1016/j.cjsc.2024.100465
5. [5]
  Peng-Fei Li , Chun-Li Hu , Bo Zhang , Jiang-Gao Mao , Fang Kong . From HgGa₂(SeO₃)₄ to Hg₂Ga(SeO₃)₂F: The first Hg^Ⅰ-based selenite birefringent crystal triggered by linear groups and fluoride ions. Chinese Chemical Letters, 2026, 37(2): 110588-. doi: 10.1016/j.cclet.2024.110588
6. [6]
  Zhenzhen Zhao , Meichen Jiao , Jiejie Ling , Han Jiang , Yan Gao , Hao Xu , Hai-Qing Li , Jingang Jiang , Peng Wu , Le Xu . Toward the microporous zeolite family with tunable large-medium cage and pore opening. Chinese Journal of Structural Chemistry, 2024, 43(9): 100336-100336. doi: 10.1016/j.cjsc.2024.100336
7. [7]
  Wei-Tong Ren , Tian Lan , Zi-Lin He , Hua-Zhe Wang , Lin Deng , Shan-Shan Ye , Qing-Lian Wu , Wan-Qian Guo . Weak electric field promotes anaerobic granular sludge formation to enhance medium-chain fatty acids synthesis. Chinese Chemical Letters, 2025, 36(8): 110563-. doi: 10.1016/j.cclet.2024.110563
8. [8]
  Xiaoke Xi , Xinpeng Li , Yang Liu , Yucheng Zhang , Linmei Li , Jianming Li , Xu Jin , Shuhong Jiao , Zhanwu Lei , Ruiguo Cao . Monolithic medium-entropy alloy electrode enables efficient and stable oxygen evolution reaction. Chinese Chemical Letters, 2025, 36(12): 110535-. doi: 10.1016/j.cclet.2024.110535
9. [9]
  Jiadong Li , Yanduo Liu , Yang Qu . Highly efficient methane-to-low alcohols conversion via ZnO based photocatalysis in aqueous medium. Chinese Chemical Letters, 2026, 37(1): 111741-. doi: 10.1016/j.cclet.2025.111741
10. [10]
  Kaili Wang , Pengcheng Liu , Mingzhe Wang , Tianran Wei , Jitao Lu , Xingling Zhao , Zaiyong Jiang , Zhimin Yuan , Xijun Liu , Jia He . Modulating d-d orbitals coupling in PtPdCu medium-entropy alloy aerogels to boost pH-general methanol electrooxidation performance. Chinese Chemical Letters, 2025, 36(4): 110532-. doi: 10.1016/j.cclet.2024.110532
11. [11]
  Bo-Ran Chang , Lin Deng , Qing-Lian Wu , Wan-Qian Guo , Hui-Ying Xue . A review: Carbon-based materials as effective additives in anaerobic fermentation, focusing on microbial chain elongation and medium chain fatty acids production. Chinese Chemical Letters, 2025, 36(7): 110411-. doi: 10.1016/j.cclet.2024.110411
12. [12]
  Zijie Lin , Qing Li . Covalent organic framework ionomers enable synergistic efficient transport of protons and oxygen in medium-temperature proton exchange membrane fuel cells. Chinese Chemical Letters, 2026, 37(1): 111784-. doi: 10.1016/j.cclet.2025.111784
13. [13]
  Huaihao CHEN , Lingwen ZHANG , Yukun CHEN , Jianjun ZHANG . A water-stable metal-organic framework probe for Al³⁺/Ga³⁺/In³⁺ detection. Chinese Journal of Inorganic Chemistry, 2025, 41(12): 2601-2608. doi: 10.11862/CJIC.20250184
14. [14]
  Honglin Gao , Chunlin Yuan , Hongyu Chen , Aiyi Dong , Pan Gao , Guangjin Hou . Surface gallium hydride on Ga2O3 polymorphs: A comparative solid-state NMR study. Chinese Journal of Structural Chemistry, 2025, 44(4): 100561-100561. doi: 10.1016/j.cjsc.2025.100561
15. [15]
  Qi Sun , Hongshan Wang , Linlin Liu , Zuxin Chen , Junjie Li , Shilie Pan . A new selenide semiconductor NaMn₃Ga₃Se₈ with strong second-harmonic generation and significant luminescence property. Chinese Journal of Structural Chemistry, 2025, 44(6): 100591-100591. doi: 10.1016/j.cjsc.2025.100591
16. [16]
  Zhen Liu , Zhi-Yuan Ren , Chen Yang , Xiangyi Shao , Li Chen , Xin Li . Asymmetric alkenylation reaction of benzoxazinones with diarylethylenes catalyzed by B(C₆F₅)₃/chiral phosphoric acid. Chinese Chemical Letters, 2024, 35(5): 108939-. doi: 10.1016/j.cclet.2023.108939
17. [17]
  Qin Wang , Han Luo , Luli Wang , Ling Huang , Liling Cao , Xuehua Dong , Guohong Zou . KSb₂F₇·2KNO₃: Unveiling the peak birefringence in inorganic antimony oxysalts. Chinese Chemical Letters, 2025, 36(7): 110173-. doi: 10.1016/j.cclet.2024.110173
18. [18]
  Jun Jiang , Hui Dai , Tao Tu . Two vicinal C(sp³)-F bonds functionalization of perfluoroalkyl halides (PFAHs). Chinese Chemical Letters, 2025, 36(7): 111054-. doi: 10.1016/j.cclet.2025.111054
19. [19]
  Xinpeng LIU , Liuyang ZHAO , Hongyi LI , Yatu CHEN , Aimin WU , Aikui LI , Hao HUANG . Ga₂O₃ coated modification and electrochemical performance of Li_1.2Mn_0.54Ni_0.13Co_0.13O₂ cathode material. Chinese Journal of Inorganic Chemistry, 2024, 40(6): 1105-1113. doi: 10.11862/CJIC.20230488
20. [20]
  Xiaorui Chen , Xuan Luo , Tongming Su , Xinling Xie , Liuyun Chen , Yuejing Bin , Zuzeng Qin , Hongbing Ji . Ga-doped Cu/γ-Al₂O₃ bifunctional interface sites promote the direct hydrogenation of CO₂ to DME. Acta Physico-Chimica Sinica, 2025, 41(10): 100126-0. doi: 10.1016/j.actphy.2025.100126

Get Citation

PDF

XML

Metrics

PDF Downloads(0)
Abstract views(1400)
HTML views(22)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Address：Zhongguancun North First Street 2,100190 Beijing, PR China Tel: +86-010-82449177-888

DownLoad: Full-Size Img PowerPoint

Return