Citation: Ye Hua SHEN, Qi Dong ZHANG, Xin Du GENG, Zhi Qing ZHANG, Yun De HOU. Purification of RhlFN-in Bacteria Bodies with Acetic Acid-Water as Mobile Phase in RPLC[J]. Chinese Chemical Letters, ;2000, 11(9): 799-802. shu

Purification of RhlFN-in Bacteria Bodies with Acetic Acid-Water as Mobile Phase in RPLC

a Institute of Modern Separation Science, Shaanxi Provincial Key Laboratory of Modern Separation Scicllce, Northwest University, Xi'an 710069;
b Institute of Virus, Chinese Academy of Preventive Medicine, Beijing 100052

Received Date: 11 February 2000

Fund Project: This work was supported by the National Natural Science Foundation of China (29675017,39880003).

The extract of E.coli containing recombinant human intcrferon-(rhIFN-) with 7.0 mol/L guanidine hydrochloride (Gu·HCl) was directly injeeted into a column of reverse phase liquid chromatography (RPLC) to scparatc and purify rhIFN-with acetic acid-water as mobile phase. Gu·HCl and most impure proteins can be scparatcd by this way.Compared with the usual dilution method, the bioactivity recovery of the purified rhIFN-was found to be over 500%.In addition, compared to common organic solvents cmploycd in RPLC, acetic acid has higher freezing point, and therefore, it is easy to concentrate the aim-irotcin by frcczc-drying when acetic acid-water is used as mobile phase in RPLC.
- Revcrscd-phasc liquid chromatography,
- recombinant hunan intcrferon-,
- purification,
- acetic acid,
- mobile phase
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