Citation: Xiang Hong LI, Yuan MA, Yi CHEN, Yu Fen ZHAO. RNA Cleavage by N-Phosphoryl Serine in Histidine Buffer[J]. Chinese Chemical Letters, ;1997, 8(1): 53-56. shu

RNA Cleavage by N-Phosphoryl Serine in Histidine Buffer

Xiang Hong LI ^a,b ,
Yuan MA ^a,b ,
Yi CHEN ^b ,
Yu Fen ZHAO ^a,b

a Bio-organic Phosophorus Chemistry Laboratory, Department of Chemistry, Tsinghua University, Beijing 100084;
b Institute of Chemistry, Chinese Academy Sciences, Beijing 100080

Received Date: 26 September 1996

Fund Project: We thank the financial support of the National Science Foundation of China and the National Science and Technology Committee.

In the presence of histidine.N-(O,O-diisopropyl) phosphoryl serine could catalyze the cleavage of RNA in aqueous solution at neutral pH.The results were detected by sub-marine agarose gel electrophoresis and capillary zone electrophoresis.It was found that at high cpncentration histidine could cleave the RNA slightly.While the participation of DIPP-Ser could significantly accelerate the cleavage reaction.N-phosphodipeptide-N-(O.O-diisopropyl) phosphoryl Ser-His are proposed to be involved in the mechanism.
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