Citation: Min ZHOU, Yong Jun MA, Xiao Yong JIN, Xiu Lan TENG, Zi Yu ZHANG, Hui CHEN. Flow-injection Chemiluminescence Determination of Trace Calf Thymus DNA[J]. Chinese Chemical Letters, ;2003, 14(10): 1051-1053. shu

Flow-injection Chemiluminescence Determination of Trace Calf Thymus DNA

Institute of Chemistry, Northwest Normal University, Lanzhou 730070

Received Date: 24 September 2002

Fund Project: We are grateful to the Natural Science Foundation of Gansu province (Project ZS001-A24-054-Y) for supporting the research work.

A flow injection procedure for the determination of calf thymus DNA (CT DNA) over the range 2.1×10^-6~2.1×10^-1 μg mL^-1 is described, based on measurement of the enhanced chemiluminescence emission of rhodamine B-Ce(Ⅳ) system, activated by imidazole-HCl buffer solution. This method is highly sensitive, with the linearity range broadened to five orders of magnitude. It has been applied to determine CT DNA in synthetic sample with satisfactory results.
- Chemiluminescence,
- flow-injection,
- calf thymus DNA,
- imidazole,
- rhodamine B
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