Citation:
Chang De ZHANG, Ling Zhi WANG, Ying Yan JIANG. IMMOBILIZATION OF GLUCOSE OXIDASE AND CELLULASE BY ENTRAPMENT OF HYDROGEN-BOND COMPLEX[J]. Chinese Chemical Letters,
;1994, 5(9): 773-776.
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Glucose oxidase (GOD) and cellulase were immobilized on the hydrogen-bond complex of chitosan and sodium carboxymethyl cellulose,The immobilized GOD (IG) can be used in a wider pH range,and its optimum temperature was increased 50℃ higher than its native state;While the optimum temperature of the immobilized cellulase (IC) was increased 20℃ The storage life of IG and IC were prolonged greatly,Both of them can be reused many times,and have a high catalytic activity,Their Michaelis constants Km were determined.
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