Citation: Zhao Peng CHEN, Jian Hui JIANG, Xiao Bing ZHANG, Guo Li SHEN, Ru Qin YU. Amplified Electrochemical Immunoassay Using HRP Labeled Protein as an Inhibitor to Silver Deposition in the Presence of H₂O₂[J]. Chinese Chemical Letters, ;2006, 17(4): 489-492. shu

Amplified Electrochemical Immunoassay Using HRP Labeled Protein as an Inhibitor to Silver Deposition in the Presence of H₂O₂

State Key Laboratory for Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082

Received Date: 9 June 2005

Fund Project: The work was financially supported by the National Natural Science Foundation of China. (Grant No. 20435010, 20375012, 20105007, 20205005).

A novel electrochemical immunosensor with amplification effect based on the enzyme inhibition of silver deposition was proposed. In this method, the capture antibody was first immobilized onto a gold electrode via a self-assembled layer. After a sandwich immunoreaction, HRP labeled antibody was bound to the gold electrode. The HRP on the electrode inhibited silver deposition when the electrode was incubated in hydroquinone-H₂O₂ solution and silver ion solution.The linear sweep voltammetry was chosen to detect the deposited silver and the result showed that the peak current was linearly proportional to the content of IgG in the range of 50 to 2500 ng/mL with a detection limit of 35 ng/mL.
- Immunosensor,
- linear sweep voltammetry,
- silver deposition
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